Signal transduci ion and control of gene expression regarding glucose metabolism in Escherichia coli

我们一直在研究大肠杆菌和酵母中与中枢代谢和细胞反应动态方面相关的调控网络。主要项目包括：1）PTS和糖酵解的调节作用; 2）tRNA介导的反式翻译的新作用; 3）蛋白质和mRNA的质量控制。以下是最近的发现：1）发现了PtsG对Mic的膜螯合机制2）发现了关于糖酵解通量控制mRNA稳定性的新机制3）发现了Lad mRNA作为tmRNA的天然靶4）发现了通过tmRNA 5）发现了mRNA质量控制发现核糖体停滞诱导的mRNA切割PtsG对Mlc的调控为研究转运蛋白如何调节核糖体的活性提供了一个很好的系统。调节蛋白对代谢状态下mRNA稳定性调节的研究才刚刚开始。核糖体停滞引起的mRNA切割为研究翻译机制的新作用提供了机会。

We have been studying the regulatory networks linked to central metabolism and dynamic aspects of the cellular response in Escherichia coli and yeast. Major projects are : 1) regulatory roles of PTS and glycolysis ; 2) new roles of tmRNA-mediated trans-translation ; 3) quality control of proteins and mRNA. The following are recent findings.1)Discoveiy of a mechanism for membrane sequestration of Mic by PtsG2)Discovery of a new mechanism regarding the control of mRNA stability by glycolytic flux.3)Discovery of Lad mRNA as a natural target for tmRNA.4)Discovery of mRNA quality control by tmRNA5)Discovery of mRNA cleavage induced by ribosome stallingRegulation of Mlc by PtsG has provided a good system to study how transporters modulate the activity of regulatory proteins. Studies on the regulation of mRNA stability in response to metabolic status have just begun. The mRNA cleavage in response to ribosome stalling is providing an opportunity to study new roles of translation machinery.