Signal transduci ion and control of gene expression regarding glucose metabolism in Escherichia coli

大肠杆菌中葡萄糖代谢的信号转导和基因表达控制

• 批准号: 13480227
• 负责人: AIBA Hiroji
• 金额: $ 9.47万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13480227/
• 关键词: glucose induction; glycolysis; glucose transporter; mRNA degradation; tmRNA; trans-translation; mRNA quality control; ribosome stalling; グルコース応答; His-リン酸リレー系; PTS; RNase E; cpsオペロン; Mlc; RNaseE; mRNAの分解

We have been studying the regulatory networks linked to central metabolism and dynamic aspects of the cellular response in Escherichia coli and yeast. Major projects are : 1) regulatory roles of PTS and glycolysis ; 2) new roles of tmRNA-mediated trans-translation ; 3) quality control of proteins and mRNA. The following are recent findings.1)Discoveiy of a mechanism for membrane sequestration of Mic by PtsG2)Discovery of a new mechanism regarding the control of mRNA stability by glycolytic flux.3)Discovery of Lad mRNA as a natural target for tmRNA.4)Discovery of mRNA quality control by tmRNA5)Discovery of mRNA cleavage induced by ribosome stallingRegulation of Mlc by PtsG has provided a good system to study how transporters modulate the activity of regulatory proteins. Studies on the regulation of mRNA stability in response to metabolic status have just begun. The mRNA cleavage in response to ribosome stalling is providing an opportunity to study new roles of translation machinery.

我们一直在研究与大肠杆菌和酵母细胞反应的中心代谢和动态方面相关的调控网络。主要项目有：1）PTS和糖酵解的调节作用； 2）tmRNA介导的反式翻译的新作用； 3）蛋白质和mRNA的质量控制。以下是最近的发现。1) 发现 PtsG 的 Mic 膜隔离机制2) 发现通过糖酵解通量控制 mRNA 稳定性的新机制。3) 发现 Lad mRNA 作为 tmRNA 的天然靶标。4) 发现 tmRNA 的 mRNA 质量控制5) 发现核糖体停滞诱导的 mRNA 裂解通过调节 Mlc PtsG 提供了一个很好的系统来研究转运蛋白如何调节调节蛋白的活性。针对代谢状态调节 mRNA 稳定性的研究才刚刚开始。响应核糖体停滞的 mRNA 裂解为研究翻译机制的新作用提供了机会。

项目成果

El-Kazzaz, W., Morita, T., Tagami, H., Inada, T., Aiba, H.: "Metabolic block at early stages of glycolytic pathway activates the Rcs phosphorelay system via increased synthesis of dTDP-glucose in Escherichia coli"Mol.Microbiol.. 51. 1117-1128 (2004)

El-Kazzaz, W.、Morita, T.、Tagami, H.、Inada, T.、Aiba, H.：“糖酵解途径早期阶段的代谢阻断通过增加大肠杆菌中 dTDP-葡萄糖的合成来激活 Rcs 磷酸传递系统

Yamamoto, Y., Sunohara, T., Jojima, K., Inada, T., Aiba, H.: "SsrA-mediated trans-translation plays a role in mRNA quality control by facilitating degradation of truncated mRNAs"RNA. 9(in press). (2003)

Yamamoto, Y.、Sunohara, T.、Jojima, K.、Inada, T.、Aiba, H.：“SsrA 介导的反式翻译通过促进截短 mRNA 的降解，在 mRNA 质量控制中发挥作用”RNA。

El-Kazzaz, W., Morita, T., Tanaka, Y, Inada, T., Aiba, H.: "Metabolic block at early stages of glycolytic pathway activates the Rcs phosphorelay system via increased synthesis of dTDP-glucose in Escherichia coli."Mol.Microbiol.. 51. 1117-1128 (2004)

El-Kazzaz, W.、Morita, T.、Tanaka, Y、Inada, T.、Aiba, H.：“糖酵解途径早期阶段的代谢阻断通过增加大肠杆菌中 dTDP-葡萄糖的合成来激活 Rcs 磷酸传递系统

Sunohara, T., Jojima, K., Yamamoto, Y., Inada, T., Aiba, H.: "Nascent peptide-mediated ribosome stalling at a stop codon induces mRNA cleavage resulting in nonstop mRNA that is recognized by tmRNA."RNA. 10. 378-386 (2004)

Sunohara, T.、Jojima, K.、Yamamoto, Y.、Inada, T.、Aiba, H.：“新生肽介导的核糖体在终止密码子处停滞会诱导 mRNA 裂解，从而产生可被 tmRNA 识别的不间断 mRNA。”

Tanaka, Y., Itoh, F., Kimata, K., Aiba, H.: "Membrane localization itself but not binding to ILCBGlc is directly responsible for the inactivation of the global repressor Mlc in Escherichia coil."Mol.Microbiol.. (in press). (2004)

Tanaka, Y.、Itoh, F.、Kimata, K.、Aiba, H.：“膜定位本身但不与 ILCBGlc 结合，直接导致了大肠杆菌中全局阻遏物 Mlc 的失活。”Mol.Microbiol..