Signal transduci ion and control of gene expression regarding glucose metabolism in Escherichia coli

我们一直在研究与大肠杆菌和酵母中的中心新陈代谢和细胞反应的动态方面有关的调控网络。主要项目有：1)PTS和糖酵解的调节作用；2)tmRNA介导的反式翻译的新作用；3)蛋白质和mRNA的质量控制。1)发现了PtsG对小鼠的膜隔离机制；G2)发现了糖酵解通量控制mRNAs稳定性的新机制；3)发现了Lad mRNAs作为tmRNAs的天然靶标；4)发现了tmRNAs对mRNAs质量的调控；5)发现了核糖体停滞引起的mRNAs切割。关于代谢状态下调节mRNA稳定性的研究才刚刚开始。核糖体停滞引起的mRNA切割为研究翻译机器的新角色提供了机会。

We have been studying the regulatory networks linked to central metabolism and dynamic aspects of the cellular response in Escherichia coli and yeast. Major projects are : 1) regulatory roles of PTS and glycolysis ; 2) new roles of tmRNA-mediated trans-translation ; 3) quality control of proteins and mRNA. The following are recent findings.1)Discoveiy of a mechanism for membrane sequestration of Mic by PtsG2)Discovery of a new mechanism regarding the control of mRNA stability by glycolytic flux.3)Discovery of Lad mRNA as a natural target for tmRNA.4)Discovery of mRNA quality control by tmRNA5)Discovery of mRNA cleavage induced by ribosome stallingRegulation of Mlc by PtsG has provided a good system to study how transporters modulate the activity of regulatory proteins. Studies on the regulation of mRNA stability in response to metabolic status have just begun. The mRNA cleavage in response to ribosome stalling is providing an opportunity to study new roles of translation machinery.