Studies on the labeling reagent for the separation science

标记试剂在仪器分析中的潜在用途是广泛的分离科学所共有的。该分子可普遍衍生化，以满足测定程序，从而提高了选择性和灵敏度。我们课题组的目标是在分离科学的各个领域合作开发合适的标记试剂。所得结果主要涉及以下领域：高效液相色谱、电分析化学、溶剂萃取分析、核酸研究、细胞膜通透性研究等。新标记试剂的典型例子如下：二胺和氨基酸的鲁米诺衍生物用于高效液相色谱和电化学检测，芘乙基和十八烷基硅化硅胶柱用于高效液相色谱测定四氯二苯并对二恶英异构体，自旋标记的寡核苷酸用于esr研究，放射性配体用于血小板血栓酶A_2受体研究，kelex 100（长烷基化氧衍生物）用于选择性溶剂萃取分析铜。

The potential use of labeling reagent in the instrumental analysis is common to the wide range of separation science. The traget molecule can be generally derivatized in order to meet the determination procedures, thus enhancing the selectivity as well as the sensitivity.Our research group had the aim to cooperate for the decelopment of the suitable labeling reagent in the various field of the separation science.The results obtained are mainly concerned with the following field; high performance liquid chromatography,electroanalytical chemistry, solvent extraction analysis, nucleic acid research, cell-membrane permeability research, etc. The typical examples of new labeled reagent are as follows; luminol-derivatives of diamines and aminoacids for HPLC and electrochemical detection, pyrenylethyl and octadecylsilylated silica gel column for HPLC determination of tetrachlorodibenzo-p-dioxin isomers, spin-labeled oligonucleotides for esr research, radioactive ligand for platelet thromboxzne A_2 receptor research, and kelex 100(long alkylated oxine derivative) for the selective solvent extraction analysis of copper.