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Study of caudate stimulation on intractable epilepsy

尾状核刺激治疗难治性癫痫的研究

基本信息

批准号：
61480311
负责人：
MORI Kazuo
金额：
$ 4.16万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1988
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61480311/
关键词：
Intractable epilepsy Premotor cortex Caudate nucleus Caudate stimulation Motor cortex キンドリング in vivo dialysis 実験てんかん皮質低頻度刺激ペニシリン焦点 DA DOPAC GABA 5-HT 低頻度持続刺激 feedback刺激

项目摘要

Roles of the caudate nucleus in seizure developments from the motor or premotor cortical foci were studied in experimental models of epilepsy. In chronic rats, continuous electrical stimulation with 8-10 Hz to the premotor cortex induced electroclinical seizures which were divided into 4 stages. Electrocoagulation or microinjection of muscimol (GABA agonist) to the ipsilateral caudate nucleus increased a stimulus threshold necessary to induce each seizure stage. Seizure developments of the premotor cortical kindling in rats were significantly suppressed following the ipsilateral caudate lesion. On the other hand, numbers of interictal spikes induced by application of penicillin to the motor cortex in acute cats were significantly decreased under the feedback stimulation (10 Hz) to the ipsilateral caudate nucleus. However, feedback caudate mucate stimulation did not modify full-blown sustained paroxysms.Electrophysiological studies suggest that striato-nigro-thalamic circuits are composed-of two successive inhibitory links (GABAergic). Also, there exist powerful caudate-thalamic inhibitory pathways. Observed inhibitory effect following lesion of, or muscimol injection to the caudate nucleus may be due to disruption of this caudate-nigral inhibitory loop in which nigro-thalamic inhibition becomes more powerful. Inhibitory effects of feedback stimulation to the caudate nucleus on interictal cortical spikes may results from the latter circuit. These findings suggest that a functions of the caudate nucleus might be changeable according to various states of seizure.Our preliminary study with in vivo microdialysis showed that measurements of extracellular neurotransmitters in the caudate nucleus during various seizure stage may be worthwhile.

在癫痫实验模型中研究了尾状核在运动或运动前皮质灶的癫痫发作发展中的作用。在慢性大鼠中，对前运动皮层进行 8-10 Hz 的连续电刺激诱发电临床癫痫发作，分为 4 个阶段。向同侧尾状核电凝或显微注射蝇蕈醇（GABA 激动剂）可增加诱发每个癫痫发作阶段所需的刺激阈值。同侧尾状核损伤后，大鼠运动前皮层点燃的癫痫发作发展受到显着抑制。另一方面，在急性猫运动皮层使用青霉素引起的发作间期尖峰数量在同侧尾状核反馈刺激（10 Hz）下显着减少。然而，反馈尾状粘液刺激并没有改变全面的持续发作。电生理学研究表明，纹状体-黑质-丘脑回路由两个连续的抑制性环节（GABA能）组成。此外，还存在强大的尾状丘脑抑制途径。尾状核损伤或注射蝇蕈醇后观察到的抑制作用可能是由于尾状黑质抑制环的破坏，其中黑质丘脑抑制变得更强大。尾状核反馈刺激对发作间期皮质尖峰的抑制作用可能是后一种回路造成的。这些发现表明，尾状核的功能可能会根据不同的癫痫发作状态而发生变化。我们对体内微透析的初步研究表明，在不同的癫痫发作阶段测量尾状核中的细胞外神经递质可能是值得的。