Studies of genetic transformation in cereal plants

用含有编码氨基糖苷类光转移酶II(APH(3‘)II)和CaMV35S启动子的嵌合基因的质粒DNA处理水稻植株。利用电穿孔或聚乙二醇法对从悬浮细胞中分离的水稻原生植株进行处理。3周后，电穿孔原生质体的栽植效率约为0.5%。选择正常对照形成的G418抗性集落进行细胞分裂，将大于L mm的愈伤组织转移到再生培养基中。从抗性愈伤组织中获得了几株再生植株。这些转化植株的叶片在含有50ug/ml卡那霉素硫酸盐的培养基中生长时不漂白，在转化植株的叶片提取液中检测到APH(3‘)II活性。转基因植株和后代的DNA分析表明，在受体植株中存在完整形式的外源DNA。还将介绍其他导致转基因植株产生的DNA转化实例。人们正在努力使外源DNA在水稻植株中整合的方式和位置可视化。

Rice plants were regenerated from protoplants treated with plasmid DNA possessing a chimeric gene encoding aminoglycoside phoshotransferase II (APH(3')II) and CaMV35S promoter. Rice protoplants isolatied from suspension cells were treated by electroporation or PEG in the presence of plasmid DNA. The planting efficiency of electroporated protplasts was about 0.5% after three weeks. The dividing cells were selected G418-resistant colonies formed in the control.Calli larger than l mm were transferred to regeneration medium. Severl plants were regenerated from drug resistant calli. Leaves of these plants were not bleached when grown in the medium containing 50 ug/ml Kanamycin sulphate APH(3')II activity was detected in the leaf extracs of transformed plants. Analysis of DNA of the trasgenic plants and progenies indicated the presence of indicated the presence of intact forms of foreign DNA in recipient plants.Other exampl of DNA transformation resulted in the production of transgenic plants will be pressented. Efforts are being made to visualize the manner and the sites of foreign DNA integration in rice plants.