分子育種基盤としてのストレス制御因子の解析

生长在一个地方的植物具有在存在各种限制的环境中生长的能力。因此，植物可能通过调节理想器官和组织的发育来调节环境。本研究从细胞死亡过程的角度研究了植物的胁迫反应。因此，我沿着这条路线使用了许多转基因植物进行研究。即分析应激反应和调节氧化还原因子的代谢产物，以及与细胞死亡因子的相互作用。研究结果表明，DFR过量生产者调节了对胁迫的反应。内部代谢物分析表明，有机物质在这些植物中改变最多，而氨基酸水平和其他代谢物没有改变。在培养的细胞中，谷胱甘肽水平高度上调，这可能是应激耐受的基础。以DFR转基因株系为例，其抗逆性的分子基础可以应用于其他育种策略，以提高植物品质。

Plants habituating one place possess the ability to grow in the environment where various constrains are exists. Thus, plants may have homeostasis to adjust environment by regulating ideal organ and tissue development. In this study, stress responses of plants were examined in view of cell death processes. Thus, I used many transgenic plants for the investigation along this line. Namely, stress responses and metabolites regulating oxidative and reductive factors, and the interaction to cell death factors were analyzed. Results obtained in this investigation indicated that DFR overproducers regulated the response to stresses. Analysis of internal metabolites suggested that organic substances were most altered in such plants, whereas amino acids levels and other metabolites ware not changed. In cultured cells, glutathione level was highly up-regulated, which may indicates the basis of stress tolerance. In the case of DFR transgenic line, molecular basis for the stress resistance may be applied to other breeding strategies to improve plant quality.