Regulation of Cholesterol Metabolism by New-Type Polyunsaturated Fatty Acids

新型多不饱和脂肪酸对胆固醇代谢的调节

• 批准号: 63470110
• 负责人: SUGANO Michihiro
• 金额: $ 4.61万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63470110/
• 关键词: Polyunsaturated fatty acids; Cholesterol; Triglyceride; Eicosanoids; Platelet aggregation; 奇数不飽和脂肪酸; 血清コレステロール; 灌流肝臓

1. Regulation of Lipid Metabolism by New-Type Polyunsaturated Fatty Acids(1) Effects of 5c,llc,14c.17c-20:4 and 5c,llc,14c-20:3 polyunsaturated fatty acids in Chinese Biota (Biota orientalis) seed oil: Either purified ethyl ester of linoleic, gamma-linolenic, alpha-linolenic acids or 20:4 acid prepared from Biota seed oil were given orally to essential fatty acid deficient rats for 10 days. From the changes in the fatty acid compositions of tissue lipids, this 20:4 acid appeared to have a potential as an essential fatty acid. The 20:4 acid exerted a specific regulatory function on lipid metabolism, in particular plasma and liver triglyceride levels. When the effect of Biota seed oil containing 8.9% of 20:4 acid and 4.8% of 20:3 acids was compared with linoleic or alpha-linolenic acid in rats fed a cholesterol-enriched diet, the seed oil efficiently prevented the elevation of plasma cholesterol and liver triglyceride. No specific effect was observed on the extent of ADP-induced platelet … More aggregation. These results indicate that Biota seed oil has a preferable effect on lipid metabolism without influencing eicosanoid production.(2) Effect of 5C,9c,12c-18:3 acid in Korean pine (Pinus koraiensis) seed oil: Under the similar feeding condition as above, pine seed oil containing 17.1% of 18:3 acid showed an intermediate effect on the plasma cholesterol level, but tended to decrease the plasma and liver triglyceride levels, compared to linoleic or alpha-linolenic acid. The seed oil neither showed any specific effect on aortic prostacyclin production nor ADP-induced platelet aggregation.(3) Metabolic effect of 5c,8c,llc.14c-19:4 acid: The 19:4 acid of the microbial origin was perfused in isolated rat liver. Compared with arachidonic acid, 19:4 acid increased production of ketone body and secretion of lipids as lipoproteins. This odd-numbered polyunsaturated fatty acid also increased secretion of cholesterol. The results suggest a specific role of 19:4 acid as a regulator of lipid metabolism.2. Analyses of eicosanoids by high-performance liquid chromatography (HPLC)The fluorescence-labeling HPLC method developed for measurement of eicosnaoids in human samples (Prostaglandins, 31:71 (1986)) was found to be applicable even to rat samples. Less

1.新型多不饱和脂肪酸对脂质代谢的调节作用（1）中国Biota（Biota orientalis）种子油中5c，llc，14 c，17 c-20：4和5c，llc，14 c-20：3多不饱和脂肪酸的作用：将纯化的亚油酸、γ-亚麻酸、α-亚麻酸乙酯或从Biota种子油中制备的20：4酸经口给予必需脂肪酸缺乏的大鼠10天。从组织脂质的脂肪酸组成的变化来看，这种20：4的酸似乎具有作为必需脂肪酸的潜力。20：4酸对脂质代谢，特别是血浆和肝脏甘油三酯水平具有特定的调节功能。当将含有8.9%的20：4酸和4.8%的20：3酸的Biota种子油的效果与亚油酸或α-亚麻酸在喂食富含胆固醇的饮食的大鼠中进行比较时，种子油有效地防止血浆胆固醇和肝脏甘油三酯的升高。对ADP诱导的血小板聚集程度无特异性影响 ...更多信息 聚合来这些结果表明，Biota籽油对脂质代谢具有较好的作用，而不影响类花生酸的产生。(2)红松（Pinus koraiensis）籽油中5C，9 C，12 C-18：3酸的作用：在与上述相似的饲养条件下，与亚油酸或α-亚麻酸相比，含有17.1% 18：3酸的松树籽油对血浆胆固醇水平显示出中等作用，但倾向于降低血浆和肝脏甘油三酯水平。种子油对主动脉前列环素的产生和ADP诱导的血小板聚集均未显示出任何特异性作用。(3)5c，8 c，11c.14c-19：4酸的代谢作用：将微生物来源的19：4酸灌注在离体大鼠肝脏中。与花生四烯酸相比，19：4酸增加了酮体的产生和脂蛋白形式的脂质的分泌。这种奇数多不饱和脂肪酸也增加了胆固醇的分泌。结果表明19：4酸作为脂质代谢的调节剂的特定作用。用高效液相色谱法（HPLC）分析类二十烷酸已发现，为测定人样品中的类二十烷酸而开发的荧光标记HPLC法（Prostaglandins，31：71（1986））甚至适用于大鼠样品。少

项目成果

M.Sugano and J.H. Lee: "Nutritional and physiological significance of lipids" J. Dispersion Sci. Technol., 10, No. 4&5, 643-665 (1989).

M.菅野和 J.H.

菅野道廣: Agric.Biol.Chem.53. (1989)

菅野道宏：Agric.Biol.Chem.53 (1989)。

I.Ikeda,J.Murakami,T.Oka,M.Sugano,H.Yamada,S.Shinmen: "Comparison of the effects of arachidonic and 5,8,11,14-nonadecatetraenoic acids on lipid secretion and ketogenesis in perfused rat liver" Lipids.

I.Ikeda,J.Murakami,T.Oka,M.Sugano,H.Yamada,S.Shinmen：“花生四烯酸和 5,8,11,14-十九碳四烯酸对灌注大鼠脂质分泌和生酮作用的比较

M.Sugano and J.H.Lee: "Nutritional and physiological significnance of lipids" J.Dispersion Science and Technology. 10. 643-665 (1989)

M.Sugano 和 J.H.Lee：“脂质的营养和生理意义”J.分散科学与技术。

I.Ikeda,J.Murakami,T.Oka,M.Sugano,H.Yamada,S.Shinmen: "Comparison of the effects of arachionic and 5、8、11、14-nonadeca tetraenoic acids on lipid secretion and ketogenesis in perfusedrat liver" Lipids(submitted).

I.Ikeda、J.Murakami、T.Oka、M.Sugano、H.Yamada、S.Shinmen：“花生四烯酸和 5,8,11,14-十九四烯酸对灌注中脂质分泌和生酮作用的影响比较大鼠肝脏”脂质（已提交）。