脂肪酸代謝の制御に対するセサミンと共役型多価不飽和脂肪酸の共同効果解析

针对模拟脂肪酸代谢的食物因子芝麻素对共轭亚油酸（CLA）体脂降能的协同作用，进行了一系列动物实验。分析血清肥胖相关细胞因子浓度及肝脂肪酸β-氧化活性，了解其作用机制。在大鼠研究中，饲粮中分别饲喂1%和0.2%水平的CLA和芝麻素对附睾脂肪组织重量有协同作用，且当饲粮中蛋白质来源为大豆蛋白而非酪蛋白时，效果明显。这种组合增加了棕色脂肪组织的重量。但对血清瘦素和TNF-α浓度无影响。饲喂CLA后，小鼠的白色脂肪组织重量和血清细胞因子浓度均显著降低，芝麻素对小鼠的影响不明显。α-亚麻酸共轭衍生物比CLA更能促进肝脏脂肪酸氧化。此外，CLA可能通过改变多不饱和脂肪酸代谢，增加脾淋巴细胞免疫球蛋白的产生。这些结果表明，尽管不同动物的反应不同，但将CLA与刺激脂肪酸代谢的食物因子适当结合，不仅可以刺激CLA降低体脂的功能，还可以产生良好的免疫状况。

Aiming at the synergistic effect of sesamin, a food factor that simulates fatty acid metabolism, on body fat reducing potential of conjugated linoleic acid(CLA), a series of animal experiments were carried out. To know the mechanism of action of this combination serum concertration of obesity-related cytokines and hepatic fatty acid β-oxidation activity were analyzed. In rat studies, feeding CLA and sesamin at dietary levels of 1% and 0.2%, respectively resulted in a synergism in the reduction of epididymal adipose tissue weight, and the effect was marked when the dietary protein source was soybean protein in relation to casein. The weight of brown adipose tissue increased with this combination. However, no effect was observed in concentration of serum leptin and TNF-α. When mice were fed CLA, the weight of white adipose tissues and concentration of serum cytokines reduced significantly, but there was no additional effect of sesamin in this animal model. The conjugated derivative of α-linolenic acid appeared to stimulate hepatic fatty acid oxidation more than that of CLA.In addition, CLA increased the production of immunoglobulins by splenic lymphocytes probably through the modification of polyunsaturated fatty acid metabolism.These results showed the possibility that, although the response differ among the animal species, an appropriate combination of CLA with food factors that stimulate fatty acid metabolism not only stimulates the function of CLA to reduce body fat, but also produces favorable immune situation.