Studies of the Immunity Regulation Mechanism of Auraptene in Citrus Peel

柑橘皮中Auraptene免疫调节机制的研究

• 批准号: 14560108
• 负责人: SUGANO Michihiro
• 金额: $ 2.11万
• 依托单位: Prefectural University of Kumamoto
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560108/
• 关键词: Auraptene; Citrus speel; Rats; TNF-α; IL-4; INF-γ; IgA; 血清トリグリセリド; インスリン; 腫瘍壊死因子; レプチン; 体脂肪

Auraptene (Aur) is a kind of coumarin compounds, occurring in the peel of specific citrus, exerts beneficial physiological functions such as anti-carcinogenic and suppression of active oxygen production. In the present study, to gain further information on physiological functions of Aur, the effects on various parameters were confirmed in rat feeding studies or lymphocyte culture system. First, using dried powders from different citrus peels either Amanatsu containing 0.01 % Aur, Ponkan essentially free from Aur or control containing cellulose were fed to rats for 4 weeks. The liver weight and the concentration of serum glucose were significantly lowered in the Amanatsu and Ponkan groups, while kidney weight and the concentration of serum TNF-α were obviously increased in rats fed Amanatsu. Then, the Aur concentrate was prepared from Amanatsu orange peel, and fed to rats 0.04 % level for 3 weeks. Aur did not affect weight gain and food efficiency, although feec intake tended to be high … More er. In the Aur group, kidney weight trended to be higher, while weight of white adipose tissue was reduced. The concentration of serum triglyceride significantly decreased in the rats fed Aur. The concentration of serum insulin trended to lower in the Aur group, however, that of serum glucose remained unchanged. There was a decreasing trend of the concentration of serum TNF-a when rats were fed Aur. When rat spleen lymphocytes were incubated with high-purity Aur prepared from Amanatsu peel, the production of IgA decreased with an increasing concentration of Aur, irrespective of the incubation time. The IgA production tended to decrease irrespective of the concentration of Aur added to the medium. The trace Aur significantly increased the production of IL-4 during 24 hours of incubation, but this effect disappeared after 72 hours. There was no obvious effect on the concentration of TNF-a, however it increased with incubation time, and the difference became significant when Aur was added in the medium. Although no clear-cut effect of Aur on the production of IFN-γ was observed, it tended to increase with incubation time. Overall, in animal experiments, it was confirmed that Aur increase the concentration of serum TNF-α. There is also a possibility that Aur affects body fat metabolism, however the effect on immune functions was not unequivocal. While in the lymphocyte experiment, the results suggested that Aur may have unique functions in the production of immunoglobulins and some cytokines. Although it is difficult to draw a distinct conclusion from the present study, Aur appears to have a potential to promote and regulate immune functions. Less

橙内酯（Auraptene，Aur）是一类存在于柑橘果皮中的香豆素类化合物，具有抗癌、抑制活性氧产生等生理功能。在本研究中，为了进一步了解Aur的生理功能，在大鼠喂养实验或淋巴细胞培养系统中证实了Aur对各种参数的影响。首先，使用来自不同柑橘皮的干燥粉末，将含有0.01% Aur的Amanatsu、基本上不含Aur的Ponkan或含有纤维素的对照物喂给大鼠4周。结果表明，鹅膏方组和对照组大鼠肝脏重量和血清葡萄糖浓度明显降低，而肾重量和血清TNF-α浓度明显升高。然后，将鹅膏橙子皮制成浓缩金，以0.04%的浓度喂饲大鼠3周. Aur不影响体重增加和食物效率，虽然feec摄入量往往很高 ...更多信息 儿在Aur组中，肾脏重量倾向于更高，而白色脂肪组织的重量减少。Aur组大鼠血清甘油三酯浓度显著降低。Aur组的血清胰岛素浓度有降低的趋势，而血糖浓度保持不变。Aur组大鼠血清TNF-α浓度呈下降趋势。当大鼠脾淋巴细胞与从天夏皮中制备的高纯度Aur孵育时，无论孵育时间如何，随着Aur浓度的增加，伊加的产生都减少。伊加的生产倾向于减少，无论浓度的Aur添加到培养基中。微量Aur在孵育24小时内显着增加IL-4的产生，但这种作用在72小时后消失。Aur对TNF-α的浓度无明显影响，但随着培养时间的延长，TNF-α的浓度逐渐升高，当Aur加入到培养液中时，差异显著。虽然Aur对IFN-γ的产生没有明显的影响，但随着孵育时间的延长，IFN-γ的产生有增加的趋势。总之，在动物实验中，证实了Aur可增加血清TNF-α的浓度。Aur也有可能影响身体脂肪代谢，但对免疫功能的影响并不明确。而在淋巴细胞实验中，结果提示Aur可能在免疫球蛋白和某些细胞因子的产生方面具有独特的功能。虽然很难从目前的研究中得出一个明确的结论，但Aur似乎具有促进和调节免疫功能的潜力。少

项目成果

Asuka AKAHOSHI, Naoko KANEDA, Asko SHIMODA, Kazuko NISHIMURA, Miyuki MINAMI, Yasuhumi KUDOH, Michihiro SUGANO: "Physiological Functions of Citrus Auraptene as Estimated by the Effects on Blood Components and Other Parameters in Rats"Journal of the Japanes

Asuka AKAHOSHI、Naoko KANEDA、Asko SHIMODA、Kazuko NISHIMURA、Miyuki MINAMI、Yasuhumi KUDOH、Michihiro SUGANO：“通过对大鼠血液成分和其他参数的影响来估计 Citrus Auraptene 的生理功能”Journal of the Japanes

赤星亜朱香, 他: "柑橘果皮およびオーラプテン濃縮物がラット血清成分および組織脂肪酸組成に及ぼす影響"日本食品科学工学会誌. 51巻3号. 161-166 (2004)

Akahoshi Asuka 等人：“柑橘皮和橙皮烯浓缩物对大鼠血清成分和组织脂肪酸组成的影响”日本食品科学技术学会杂志，第 51 卷，第 3 期。161-166（2004 年）。