Nutritional and Physiological Assessment of trans-Monoene Fatty Acids in Hydrogenated Fat Products

氢化脂肪产品中反式单烯脂肪酸的营养和生理学评估

• 批准号: 60303023
• 负责人: SUGANO Michihiro
• 金额: $ 5.38万
• 依托单位: Kyushu University, Faculty of Agriculture
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60303023/
• 关键词: trans-Fatty acids; Cholesterol; Gallstone; Atherosclerosis; Platelet aggregation; Eicosanoids; Intestinal flora; 乳癌・結腸癌; 胆汁酸; 血圧

A gas-liquid chromatographic method for determination of geometrical isomers of fatty acids was developed using a SS-10 capillary column. The GC-MS method was found to be useful for the rapid analysis of positional isomers. The prototype of trans-fatty acids in home-use margarines was trans-octadecenoates comprising on the average 10% of total fatty acids and only negligible amounts of the polyunsaturated fatty acid isomers occurred. Hydrogenated products for institutional use contained a high proportion of trans-isomers. Trans-octadecenoates slightly modified the constitution of intestinal microflora of rats, and an enhanced conversion of cholesterol to coproderivatives could be attributed to the change in enzymatic activity. Trans-fatty acids were utilized as an energy source as effectively as the cis-counterparts. There was no specific deposition of trans-isomers in rat tissues and they rapidly disappeared on discontinuance of ingestion. Trans-eicosenoates and dococenoates in hydrogenated marine oil did not deposite in rat tissues. Trans-fatty acids reduced the proportion of polyunsaturated fatty acids in rat heart mitochondrial phospholipids and depressed respiratory activity when the supply of linoleic acid was marginal. Trans-fatty acids did not stimulate the formation of cholesterol gallstone in mice and also reduced a lithogenic index in hamsters. In various animals, trans-fatty acids reduced plasma cholesterol levels and the atherogenic activity in quails was lower in hydrogenated fat than in unhydrogenated fats. Although trans-fatty acids depressed platelet aggregation in rats, the production of major eicosanoids remained unchanged. Trans-fatty acid promoted neither carcinogen-induced mammary tumors nor colon tumors in rats.

采用SS-10毛细管色谱柱，建立了脂肪酸几何异构体的气液色谱分析方法。GC-MS方法可用于位置异构体的快速分析。家用人造黄油中反式脂肪酸的原型是反式十八碳烯酸酯，其平均占总脂肪酸的10%，并且仅存在可忽略不计量的多不饱和脂肪酸异构体。供机构使用的氢化产品含有很高比例的反式异构体。反式十八碳烯酸酯轻微改变了大鼠肠道菌群的构成，并且胆固醇向粪衍生物的转化增强可归因于酶活性的变化。反式脂肪酸被有效地用作能量来源的顺式对应物。有没有具体的沉积反式异构体在大鼠组织中，他们迅速消失，停止摄入。氢化海洋油中的反式二十碳烯酸酯和二十二碳烯酸酯在大鼠组织中没有沉积。反式脂肪酸降低了大鼠心脏线粒体磷脂中多不饱和脂肪酸的比例，并在亚油酸供应不足时抑制呼吸活动。反式脂肪酸不会刺激小鼠胆固醇结石的形成，也会降低仓鼠的成石指数。在各种动物中，反式脂肪酸降低血浆胆固醇水平，氢化脂肪中鹌鹑的致动脉粥样硬化活性低于未氢化脂肪。虽然反式脂肪酸抑制大鼠血小板聚集，但主要类花生酸的产生保持不变。反式脂肪酸既不会促进致癌物诱导的大鼠乳腺肿瘤，也不会促进大鼠结肠肿瘤。

项目成果

T. Ide and M. Sugano: trans Fat and Cholesterol Metabolism: Fact and False, In "Nutritional Effects on Cholesterol Metabolism, Edited by Anton C.Beynen. Transmondial, Voorthuizen, 47-66 (1986)

T. Ide 和 M. Sugano：反式脂肪和胆固醇代谢：事实与错误，见“营养对胆固醇代谢的影响”，Anton C.Beynen 编辑。Transmondial，Voorthuizen，47-66 (1986)

Y.-J. Cho; T. lde and M. Sugano: J. Nutr. Sci. Vitaminol.32. 479-506 (1986)

Y.-J。

S. Yamaoka, R. Urade and M. Kitoh: "Mitochondrial Function in Rats is Affected by Modification of Membrane Phospholipids with Dietary Sardine Oil" J. Nutrition. 118. (1988)

S. Yamaoka、R. Urade 和 M. Kitoh：“膳食沙丁鱼油对膜磷脂的修饰会影响大鼠的线粒体功能”J. Nutrition。

S. Yamaoka, R. Urade and M. Kitoh: J. Nutrition. 118. (1988)

S. Yamaoka、R. Urade 和 M. Kitoh：J. 营养学。

岡本隆久,知見憲次,丸山武紀,兼松弘,新谷〓,志村光雄,亀井正治,藤田忠雄,神戸保,佐々木清司,管野道広: 油化学. 37. (1988)

Takahisa Okamoto、Kenji Kenji、Takeki Maruyama、Hiroshi Kanematsu、Shintani、Mitsuo Shimura、Masaharu Kamei、Tadao Fujita、Tamotsu Koho、Seiji Sasaki、Michihiro Kanno：石油化学。（1988）