有害金属の電離状態が細胞毒性に及ぼす影響と許容濃度との関係に関する衛生学的研究

有毒金属电离态对细胞毒性影响与允许浓度关系的卫生学研究

• 批准号: 63480176
• 负责人: SAKURAI Haruhiko
• 金额: $ 3.39万
• 依托单位: Department of Preventive Medicine and Public Health, School of Medicine, Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63480176/
• 关键词: threshold limit value; chromium compounds; platinum compounds; transformation; R B C ghost; cell fusion; secondary screening test; 金属アレルギー; 感作性; 金属耐性; 細胞融合; 金属毒性

We conducted experimental studies for metal compounds in which the metal can take several electric charge. Using C3HlOT1/2 and BALB/3T3 established mouse cell line, cytotoxicity and transformation inducibility of chromium acetate and chromium trioxide were examined. Dose-response relationship of cytotoxicity was more conspicuous in C3H10T1/2 cell line. And it is suggested that the carcinogenic activity of the hexavalent compound is much stronger than that of the trivalent compound and twice or three times as high as that of 20-Methylcholanthrene, a positive control agent.The cytotoxicity of the hexavalent chromium compound after cell fusion of L-B82 cells and RBC ghosts were examined by the electronic cell fusion technique. The survival rate of cells was lowest in the group exposed to chromium trioxide and cultured in a serum-free medium.In the experiment to detect the allergic induction of platinum compounds to human lymphocyte by the production of IgE, sodium tetrachloroplatinate showed more inducibility than sodium hexachloroplatinate.Threshold limit values should be decided not only by the results of in vitro studies, but by the conditions of actual exposure and epidemiological informations. It is true that a series of tests performed this time have some problem in quantitativeness and reproducibility, but they could be new kinds of secondary screening tests after the solution of these problems.

我们对金属化合物进行了实验研究，其中金属可以带几个电荷。用已建立的小鼠C3 H1 OT 1/2和BALB/3 T3细胞系，研究了醋酸铬和三氧化铬的细胞毒性和转化诱导作用。对C3 H10 T1/2细胞的杀伤作用呈剂量-反应关系。用电子细胞融合技术检测了六价铬化合物对L-B_（82）细胞和红细胞血影细胞融合后的细胞毒性。三氧化铬暴露组和无血清培养组的细胞存活率最低。在检测铂类化合物对人淋巴细胞产生IgE的变态反应诱导作用的实验中，四氯铂酸钠的诱导作用强于六氯铂酸钠。阈值的确定不仅应根据体外研究结果，而是由实际暴露条件和流行病学信息决定的。这次进行的一系列试验在定量性和重现性上确实存在一些问题，但在解决这些问题后，它们可以成为新的二次筛选试验。

项目成果

桜井治彦: "西ドイツにおける許容濃度" 産業医学レビュ-. 1. 14-24 (1988)

Haruhiko Sakurai：“西德的耐受浓度”工业医学评论 1. 14-24 (1988)。

IARC/NCI/EPA Working group: "Cellular and molecular mechanisms of cell transformation and standardization of transformation assays of established cell lines for the prediction of carcinogenic chemicals: Overview and recommended protocols." Cancer Research

IARC/NCI/EPA 工作组：“细胞转化的细胞和分子机制以及用于预测致癌化学物质的已建立细胞系转化测定的标准化：概述和推荐方案。”

Khan, A., Hill, J.M., Grater W., Loeb, E., Mclellan and Hill, N.: "Atopic hypersensitivity to cis-dichlorodiammmine-platinum(II) and other platinum complexes." Cancer Res. 35: 2766-2700, 1975.

Khan, A.、Hill, J.M.、Grater W.、Loeb, E.、Mclellan 和 Hill, N.：“对顺式二氯二氨铂 (II) 和其他铂络合物的特应性超敏反应。”

Norseth T.: "The carcinogenicity of chromium." Environ. Health Perspectives 40: 121-130, 1981.

Norseth T.：“铬的致癌性。”

Parrot, J.L., Hebert, R., Saindelle A. and Ruff, F.: "Platinum and platinosis: Allergy and histamine releatium salts." Arch. Environ. Health 19: 685-691, 1969.

Parrot, J.L.、Hebert, R.、Saindelle A. 和 Ruff, F.：“铂和铂沉着症：过敏和组胺相关盐。”