Postnatal alterations of mechanisms of signal transduction in rat salivary glands

大鼠唾液腺信号转导机制的出生后改变

• 批准号: 03807123
• 负责人: ISHIKAWA Yasuko
• 金额: $ 1.22万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03807123/
• 关键词: Parotid glands; Cell membrane; beta-adrenoceptors; Heterotrimetric G proteins; Monomeric G proteins; Secretory granules; Cell differentiation; Developmental changes; β-アドレナリン受容体; GTP結合蛋白質; アデニル酸シクラーゼ; c-AMP; アミラーゼ分泌; チモーゲン顆粒; 分化・成長; βー受容体 /

The postnatal alteration of the function of rat parotid glands was studied in relation to that of BETA adrenoceptor-adenylate cyclase system. The tissues of 1-week-old rats responded to isoproterenol(IPR) and caused amylase secretion. The EC_<50> value of the tissues for IPR in amylase secretion was ten time higher than that of adult rats. The tissues also responded to forskolin(FOR) and markedly caused amylase secretion. However, the secretory response of the tissues to FOR decreased during development. Binding experiments of [^3H]dihydroalprenolol(DHA) show that the specific binding of the ligand was already detected in parotid tissues of 3-day-old rats and Bmax value increased steadily until 4 week after birth when it leveled off to that of the tissues of adult rats. The IC_<50> values of IPR for [^3H]DHA binding in the tissues of 1-and 8-week old rats were 890 (〕SY.+-.〔) 74.2 and 3.5 (〕SY.+-.〔) 0.8muM, respectively. But the value of propranolol did not show any changes during devel … More opment. The c-AMP content in the tissues of 1-week-old rats also increased by the treatment with IPR as observed in adult rat tissues. The increasing ratio in the tissues of adult rats by IPR was twice as that of 1-week-old rats. FOR also caused remarkable increases in c-AMP content, but the response markedly decreased during development and paralleled amylase secretion. Binding experiments of [^<35>S]GTPgammaS showed the presence of GTP binding capacity of G proteins in the tissues of 1-week-old rats. The Bmax value of specific [^<35>S]GTPgammaS binding in the tissues in creased steadily during development, reaching plateau at 4 weeks after birth. The GTP binding capacities of Gs proteins and Gi proteins showed the same developmental increases.These results show that the function of beta-adrenoceptor-adenylate cyclase system in rat parotid tissues is markedly enhanced during development and the superior efficacy of the beta-adrenergic stimuli for the induction of amylase secretion is to be found in adult rats. Less

本文研究了大鼠生后腮腺功能的变化与β肾上腺素受体-腺苷酸环化酶系统的关系。1周龄大鼠的组织对异丙肾上腺素（IPR）有反应，并引起淀粉酶分泌。IPR<50>组织淀粉酶分泌的EC值比成年大鼠高10倍。这些组织也对毛喉素（FOR）有反应，并明显引起淀粉酶分泌。然而，在发育过程中，组织对FOR的分泌反应下降。[^3H]二氢烯丙洛尔（DHA）的结合实验表明，3日龄大鼠腮腺组织中已检测到该配体的特异性结合，Bmax值稳定增加，直至生后4周，该值与成年大鼠组织中的Bmax值持平。1<50>周龄和8周龄大鼠组织中[^3H]DHA结合的IPR IC_（1/2）值为890 SY ±-。（）74.2和3.5（）SY.（）0.8 μ M。但普萘洛尔的值在发育过程中未显示任何变化， ...更多信息 opment. 1周龄大鼠组织中的c-AMP含量也因IPR处理而增加，与成年大鼠组织中观察到的一样。IPR在成年大鼠组织中的增加率是1周龄大鼠的2倍。FOR还引起c-AMP含量的显著增加，但在发育和β-淀粉酶分泌过程中，这种反应明显减弱。[^<35>S] GTP γ S的结合实验表明，在1周龄大鼠的组织中存在G蛋白的GTP结合能力。各组织中特异性[^<35>S] GTP γ S结合的Bmax值在发育过程中逐渐增加，出生后4周达到平台。Gs蛋白和Gi蛋白的GTP结合量在发育过程中也有增加，表明大鼠腮腺组织中β-肾上腺素能受体-腺苷酸环化酶系统的功能在发育过程中明显增强，β-肾上腺素能刺激物诱导淀粉酶分泌的上级效应在成年大鼠中也存在。少

项目成果

Yasuko Ishikawa: Circadian rhythm and salivary glands (JAPANESE). Ishiyaku Publishers, Inc., DENTAL OUTLOOK 80,1 (1992)

Yasuko Ishikawa：昼夜节律和唾液腺（日语）。

Y.Ishikawa: "Characteristics of proteins secreted by isoproterenol from rat parotie tissues treated briefly with the agonist." The Japanese Journal of Pharmacology. 58. 218 (1992)

Y.Ishikawa：“用激动剂短暂处理的大鼠腮腺组织中异丙肾上腺素分泌的蛋白质的特征。”

Y.Ishikawa: "ラット唾液腺薬物受容体の生後変化" 日本唾液腺学会誌. 32. 66-69 (1991)

Y. Ishikawa：“大鼠唾液腺药物受体的产后变化”日本唾液腺学会杂志 32. 66-69 (1991)。

Yasuko Ishikawa: "Age-dependent changes in adenylul cyclase and GTP-binding proteins in parotid gland." (in preparation).

Yasuko Ishikawa：“腮腺中腺苷酸环化酶和 GTP 结合蛋白的年龄依赖性变化。”

Yasuko Ishikawa: "Mechanism of isoproterenol-induced heterologous desensitization of mucin secretion from rat submandibular glands:" Biochimica et Biophysica Acta. (in press). (1994)

Yasuko Ishikawa：“异丙肾上腺素诱导大鼠颌下腺粘蛋白分泌异源脱敏的机制：”生物化学和生物物理学学报。