Effects of DNA repair on homologous and non-homologous recombination
Effects of DNA repair on homologous and non-homologous recombination
批准号:
04640592
负责人:
INOUE Hirokazu
金额:
$1.09万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for General Scientific Research (C)
财政年份:
1992
资助国家:
日本
项目状态:
已结题
起止时间:
1992 至 1993
中文摘要
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英文摘要
About 40 different Neurospora DNA repair mutants were characterized genetically and their sensitivities to mutagens were extensively tested. From those results, we isolated 5 to 6 as candidates of recombinational repair-deficient mutants and tested their abilities of integration of a plasmid pMTR to genome DNA.Mutants, mei-3, mei-2, uvs-6, mus-25 showed very low homologous recombination frequencies. We tried to clone those genes using sib selection method. Cloned mei-3 showed that it has high similarity to E.coli recA and S.cerevisiae RAd51. Gene mus-25 was a homolog of S.cerevisiae RAD54. They are member of recombinational repair in yeast. Therefore, it seems that this group including mei-3 and mus-25, functions in homologous recombination system in Neurospora crassa.
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H.Tomita: "The neurospora uvs-2 gene encodes a protein which has homology to yeast Rad18,with unique zinc finger motifs" Mol Gen Genet. 238. 225-233 (1993)
H.Tomita:“脉孢菌 uvs-2 基因编码的蛋白质与酵母 Rad18 有同源性,具有独特的锌指基序”Mol Gen Genet。
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S.Hatakeyama, C.Ishii and H.Inoue: "Identification and expression of the Neurospora crassa mei-3 gene which encodes a protein homologous to Rad51 of Saccharomyces cerevisiae" Mol Gen Genet. 249. 439-446 (1995)
S.Hatakeyama、C.Ishii 和 H.Inoue:“粗糙脉孢菌 mei-3 基因的鉴定和表达,该基因编码与酿酒酵母 Rad51 同源的蛋白质”Mol Gen Genet。
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C.Ishii: "Mutagenesis depistatic grouping of the Neurospora meiotic mutants,mei-2 and mei-3,which are sensitive to mutagens" Mutation Res. 315. 249-259 (1994)
C.Ishii:“对诱变剂敏感的脉孢菌减数分裂突变体 mei-2 和 mei-3 的诱变 depistatic 分组”突变研究。
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K.Watanabe: "Genetic and molecular characterization of Neurospora crassa mus-23" Mol Gen Genet. 256. (1997)
K.Watanabe:“粗糙脉孢菌 mus-23 的遗传和分子特征”Mol Gen Genet。
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S.Hatakeyama: "Identification and expression of the Neurospora crassa mei-3 gene which encodes a protein homologous to Rad51 of Saccharomvces cerevisi" Mol Gen Genet. 249. 439-446 (1995)
S.Hatakeyama:“粗糙脉孢菌 mei-3 基因的鉴定和表达,该基因编码与酿酒酵母的 Rad51 同源的蛋白质”Mol Gen Genet。
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