Effects of DNA repair on homologous and non-homologous recombination

DNA 修复对同源和非同源重组的影响

• 批准号: 04640592
• 负责人: INOUE Hirokazu
• 金额: $ 1.09万
• 依托单位: Saitama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04640592/
• 关键词: homologous recombination; Neurospora crassa; non-homologous recombination; cloning; mei-3; RAD51; DNA repair; recombinational repair; 相同的組み換え; 非相同的組み換え; DNA修復欠損株

About 40 different Neurospora DNA repair mutants were characterized genetically and their sensitivities to mutagens were extensively tested. From those results, we isolated 5 to 6 as candidates of recombinational repair-deficient mutants and tested their abilities of integration of a plasmid pMTR to genome DNA.Mutants, mei-3, mei-2, uvs-6, mus-25 showed very low homologous recombination frequencies. We tried to clone those genes using sib selection method. Cloned mei-3 showed that it has high similarity to E.coli recA and S.cerevisiae RAd51. Gene mus-25 was a homolog of S.cerevisiae RAD54. They are member of recombinational repair in yeast. Therefore, it seems that this group including mei-3 and mus-25, functions in homologous recombination system in Neurospora crassa.

对大约40种不同的神经孢子虫DNA修复突变体进行了遗传鉴定，并对它们对诱变剂的敏感性进行了广泛的测试。从这些结果中，我们分离了5到6个重组修复缺陷突变体作为候选突变体，并测试了它们将质粒pMTR整合到基因组DNA的能力。突变体mei-3、mei-2、uv -6、mu -25的同源重组频率非常低。我们尝试用同胞选择的方法克隆这些基因。克隆的mei-3与E.coli recA和S.cerevisiae RAd51具有较高的相似性。基因mu -25与酿酒酵母RAD54同源。它们是酵母中重组修复的成员。因此，包括mei-3和mu -25在内的这一基团似乎在粗神经孢子虫的同源重组系统中起作用。

项目成果

H.Tomita: "The neurospora uvs-2 gene encodes a protein which has homology to yeast Rad18,with unique zinc finger motifs" Mol Gen Genet. 238. 225-233 (1993)

H.Tomita：“脉孢菌 uvs-2 基因编码的蛋白质与酵母 Rad18 有同源性，具有独特的锌指基序”Mol Gen Genet。

S.Hatakeyama, C.Ishii and H.Inoue: "Identification and expression of the Neurospora crassa mei-3 gene which encodes a protein homologous to Rad51 of Saccharomyces cerevisiae" Mol Gen Genet. 249. 439-446 (1995)

S.Hatakeyama、C.Ishii 和 H.Inoue：“粗糙脉孢菌 mei-3 基因的鉴定和表达，该基因编码与酿酒酵母 Rad51 同源的蛋白质”Mol Gen Genet。

C.Ishii: "Mutagenesis depistatic grouping of the Neurospora meiotic mutants,mei-2 and mei-3,which are sensitive to mutagens" Mutation Res. 315. 249-259 (1994)

C.Ishii：“对诱变剂敏感的脉孢菌减数分裂突变体 mei-2 和 mei-3 的诱变 depistatic 分组”突变研究。

K.Watanabe: "Genetic and molecular characterization of Neurospora crassa mus-23" Mol Gen Genet. 256. (1997)

K.Watanabe：“粗糙脉孢菌 mus-23 的遗传和分子特征”Mol Gen Genet。

S.Hatakeyama: "Identification and expression of the Neurospora crassa mei-3 gene which encodes a protein homologous to Rad51 of Saccharomvces cerevisi" Mol Gen Genet. 249. 439-446 (1995)

S.Hatakeyama：“粗糙脉孢菌 mei-3 基因的鉴定和表达，该基因编码与酿酒酵母的 Rad51 同源的蛋白质”Mol Gen Genet。