Analysis of microtubule-organizing centers (MTOCs) in Aspergillus nidulans and Neurospora crassa

构巢曲霉和粗糙脉孢菌中微管组织中心 (MTOC) 的分析

• 批准号: 351340833
• 负责人: Professor Dr. Reinhard Fischer
• 金额: --
• 依托单位: Departamento de Microbiología
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/351340833?language=en
• 关键词: Analysis; microtubule; organizing; centers; MTOCs

Microtubule formation in eukaryotic cells depends on large, multi-subunit protein complexes, called Microtubule-organizing centres (MTOCs). One extremely well-studied MTOC is the one in Saccharomyces cerevisiae, where the MTOC is embedded into the nuclear envelope and named spindle-pole body (SPB). In Schizosaccharomyces pombe three different MTOCs generate the MT arrays during different stages of the cell cycle, multiple interphase iMTOCs located in the nuclear envelope, the SPBs, and equatorial, eMTOCs, during cyokinesis. All three are spatially and temporally regulated. In the filamentous fungus Aspergillus nidulans, in which gamma-tubulin was discovered, two stable MTOCs were described, the SPBs and septal MTOCs (sMTOCs). We found that sMTOCs are anchored at septa through the disordered protein Spa10, and that the protein composition resembles the one of the outer plaque of SPBs. In Neurospora crassa SPBs polymerize mitotic and cytoplasmic MTs, but in addition there is evidence for MTOCs in the growing tip in the cytoplasm. Nothing is known about the biochemical composition and the activity. Here, we propose to perform a comparative study of the MTOCs in A. nidulans and N. crassa. The role of several proteins, such as MOZART 1 (MztA and MTZ-1) or the J-domain containing protein RspA, will be studied. Besides the biochemical composition of MTOCs, the hierarchy of the assembly will be unraveled. In addition, non-targeted approaches, such as BioID or GFP-trap technology, will be undertaken to discover novel structural and regulatory components.

真核细胞中微管的形成依赖于大的多亚基蛋白复合物，称为微管组织中心（MTOC）。一种研究非常充分的MTOC是酿酒酵母中的MTOC，其中MTOC嵌入核膜中，并命名为纺锤体（SPB）。在粟酒裂殖酵母中，三种不同的MTOC在细胞周期的不同阶段产生MT阵列，多个间期iMTOC位于核膜中，SPB和赤道，eMTOC，在细胞分裂期间。所有这三个都是空间和时间调节的。在发现γ-微管蛋白的丝状真菌构巢曲霉（Aspergillus nidulans）中，描述了两种稳定的MTOC，SPB和隔MTOC（sMTOC）。我们发现sMTOC通过无序的蛋白Spa 10锚定在隔膜上，并且蛋白质组成类似于SPB的外部斑块之一。在粗糙脉孢菌SPBs中，除了有丝分裂和细胞质MT外，还有证据表明细胞质中的生长尖端中存在MTOC。关于其生化组成和活性还不清楚。在这里，我们建议进行比较研究的MTOC在A。nidulans和N.粗鲁。将研究几种蛋白质，如MOZART 1（MztA和MTZ-1）或含J结构域的蛋白质RspA的作用。除了MTOC的生化组成外，组装的层次结构也将被揭开。此外，还将采用非靶向方法，如BioID或GFP捕获技术，以发现新的结构和调控成分。

项目成果

Aspergillus nidulans als Modellsystem für filamentöse Pilze

构巢曲霉作为丝状真菌的模型系统

• DOI: 10.1007/978-3-662-54868-4_4
• 发表时间: 2019
• 期刊: Modellorganismen
• 作者: Fischer R

The role of Aspergillus nidulans polo-like kinase PlkA in microtubule-organizing center control.

• DOI: 10.1242/jcs.256537
• 发表时间: 2021-07
• 期刊: Journal of cell science
• 影响因子: 4
• 作者: Xiaolei Gao;S. Herrero;Valentin Wernet;S. Erhardt;O. Valerius;G. Braus;R. Fischer

Application of PALM Superresolution Microscopy to the Analysis of Microtubule-Organizing Centers (MTOCs) in Aspergillus nidulans.

PALM 超分辨显微镜在构巢曲霉微管组织中心 (MTOC) 分析中的应用

• DOI: 10.1007/978-1-0716-1538-6_20
• 发表时间: 2021
• 期刊: Methods in molecular biology
• 作者: Fischer R;Takeshita N
• 通讯作者: Takeshita N

The spindle pole body of Aspergillus nidulans is asymmetrical and contains changing numbers of γ-tubulin complexes

• DOI: 10.1242/jcs.234799
• 发表时间: 2019-12-15
• 期刊: JOURNAL OF CELL SCIENCE
• 影响因子: 4
• 作者: Gao, Xiaolei;Schmid, Marjorie;Fischer, Reinhard
• 通讯作者: Fischer, Reinhard

The Cytoskeleton and Polarity Markers During Polarized Growth of Filamentous Fungi

• DOI: 10.1007/978-3-030-05448-9_3
• 发表时间: 2019
• 期刊: Biology of the Fungal Cell
• 作者: Norio Takeshita;R. Fischer