Characterization of the mus-10 and recQ genes involving in DNA repair, recombination, and senescence

参与 DNA 修复、重组和衰老的 mus-10 和 recQ 基因的表征

• 批准号: 11640619
• 负责人: INOUE Hirokazu
• 金额: $ 2.3万
• 依托单位: Saitama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11640619/
• 关键词: recQ; DNA repair; Recombination; Neurospora; queling; mitochondria; Instability; aging; mus-10; ミトコンドリアDNA; DNAシークエンス

Neurospora crassa has 2 recQ homolog genes, recQ1 and recQ2, which encode RecQ helicase. We disrupted those genes and characterized phenotypes. Gene recQ1 was same to mus-19 and also qde-3 genes. The mus-19 mutant is sensitive to MMS. EMS, MNNG, 4NQO and camptotecin. Epistasis analyzes showed that mus-19 belonged to recombination repair and postreplication repair. It did not show high mutation frequency and high recombination frequency. In the mus-19 strain we could not identify "quering" that is gene silencing in N. crassa. Also aging of the hyphae was not observed in this strain. Like the mus-19 strain, the recQ2 mutant was sensitive to mutagens. We are investigating other phenotype of this strain.The mus-10 mutant grow normally after germination, but several days later the growth became slow and finally stopped. This strain was sensitive UV and MMS. Epistasis analyzes showed that combination with mus-18 become synergistic in mutagen sensitivity, but other combination did not. Telomere length did not change at the aging step. Some data showed this mutant had defect in mitochondria DNA stability.

粗糙脉孢菌有两个RecQ同源基因，即recQ1和recQ2，编码RecQ解旋酶。我们破坏了这些基因，并对其表型进行了分析。RecQ1基因与MUS-19相同，QDE-3基因与MUS-19相同。MUS-19突变体对MMS敏感。EMS、MNNG、4NQO和喜树碱。上位性分析表明，MUS-19属于重组修复和复制后修复。未表现出高突变率和高重组率。在MUS-19菌株中，我们不能确定“quering”，也就是在n.crassa中的基因沉默。在该菌株中也没有观察到菌丝的老化。与MUS-19菌株一样，recQ2突变株对诱变剂敏感。我们正在研究该菌株的其他表型。MUS-10突变株在萌发后正常生长，但几天后生长变慢，最终停止生长。该菌株对UV和MMS敏感。上位性分析表明，MUS-18与MUS-18联用对诱变剂的敏感性有协同作用，而其他联用对诱变剂敏感性无协同作用。端粒长度在老化过程中没有变化。有资料表明该突变体存在线粒体DNA稳定性缺陷。

项目成果

A.Kato, et al.: "Characterization of a recQ homolog in Neurospora crassa"Gene & Genetic Systems. 76. 389 (2000)

A.Kato 等人：“粗糙脉孢菌中的 recQ 同源物的特征”基因

Sawada, S., M. Kobori, S. Hatakeyama, H. Inoue.: "Analyses of the mutagen-sensitive mutant, mus-10, in Neurospora crassa"Genes & Genetic Systems. 74 (6). 329 (1999)

Sawada, S., M. Kobori, S. Hatakeyama, H. Inoue.：“粗糙脉孢菌中诱变剂敏感突变体 mus-10 的分析”基因

A.Kato, et al.: "Cloning and charaterization of a RECQ homolog in Neurospora crassa"Gene & Genetic Systems. 74. 329 (1999)

A.Kato 等人：“粗糙脉孢菌中 RECQ 同源物的克隆和表征”基因

Kato, A., Y. Akamatsu, Y. Sakuraba H. Inoue: "Characterization of a recQ homologue in Neurospora crassa : involvement in recombination and gene silencing"Genes & Genetic Systems. 76 (6). 389 (2000)

Kato, A.、Y. Akamatsu、Y. Sakuraba H. Inoue：“粗糙脉孢菌中 recQ 同源物的表征：参与重组和基因沉默”基因

S.Sawada, et al.: "Analysis of the mutagen-sensitive mutant, mus-10, in Neurospora crassa"Gene & Genetic Systems. 74. 329 (1999)

S.Sawada 等人：“粗糙脉孢菌中诱变剂敏感突变体 mus-10 的分析”基因