Establishment of immunoassays for insulin-like peptide 3 in canine and bovine blood and their application for examination of testicular functions

犬和​​牛血液中胰岛素样肽3免疫分析方法的建立及其在睾丸功能检测中的应用

• 批准号: 22580365
• 负责人: KAWATE Noritoshi
• 金额: $ 3万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580365/
• 关键词: 臨床繁殖; 産科; INSL3; イヌ; ウシ; 潜在精巣; テストステロン; ライディッヒ細胞; EIA法; TR-FIA法; 雄イヌ; 雄ウシ; 性成熟過程; 精巣; 精巣間質細胞(ライディッヒ細胞); 酵素免疫測定法(EIA法)

We established immunoassays for canine and bovine insulin-peptide 3 (INSL3) and examined changes of the hormone secretion during sexual maturation and aging in male animals. We also analyzed the INSL3 secretion in cryptorchid cases to examine pathological condition and application to its diagnosis. As the results, the change of INSL3 concentration differed from those of testosterone during puberty. It is elucidated that endocrine function of testicular Leydig cells is reduced in cryptorchid cases. Also, it is suggested that retained testis can be predicted by measuring INSL3 in dogs without testis in scrotum.

我们建立了犬和牛胰岛素肽3（INSL 3）的免疫测定法，并检查了雄性动物性成熟和衰老过程中激素分泌的变化。我们还分析了隐睾病例中INSL 3的分泌，以检查病理状态及其在诊断中的应用。结果表明，青春期INSL 3浓度的变化与睾酮的变化不同。阐明隐睾患者睾丸间质细胞的内分泌功能降低。此外，这表明，保留睾丸可以通过测量INSL 3在阴囊中没有睾丸的狗预测。

项目成果

Comparison of Plasma Concentrations of Estradiol-17β and Progesterone, and Conception in Dairy Cows with Cystic Ovarian Diseases between Ovsynch and Ovsynch plus CIDR Timed AI Protocols.

Ovsynch 和 Ovsynch 加 CIDR 定时 AI 方案之间雌二醇-17β 和孕酮的血浆浓度以及患有囊性卵巢疾病的奶牛受孕的比较。

• 发表时间: 2011
• 期刊: Journal of Reproduction and Development 57
• 作者: Kawate N;Watanabe K;Uenaka K;Takahashi M;Inaba T;Tamada H
• 通讯作者: Tamada H

Effects of estrogen and phthalates on testosterone secretion in cultured testicular cells from cryptorchid and normal dogs

雌激素和邻苯二甲酸盐对隐睾和正常犬培养睾丸细胞睾酮分泌的影响

• 发表时间: 2010
• 作者: Pathirana IN;Tanaka K;Tsuji M;Hatoya S;Inaba T;Takahashi M;Tamada H;Kawate N.
• 通讯作者: Kawate N.

Detection of Relaxin mRNA in the Corpus Luteum, Uterus, and Uterine Cervix in the Bitch

母犬黄体、子宫、宫颈松弛素mRNA的检测

• DOI: 10.1292/jvms.09-0485
• 发表时间: 2010
• 期刊: Journal of Veterinary Medical Science
• 影响因子: 1.2
• 作者: Tamada H;Kawate N;Kawata N;Inaba T;Kida K;Hatoya S;Akune A;Nakama K;Kohsaka T;Sawada T
• 通讯作者: Sawada T

Effect of insulin-like peptide 3 on testosterone secretion and cAMP release in mouse Leydig cells.

胰岛素样肽 3 对小鼠 Leydig 细胞睾酮分泌和 cAMP 释放的影响。

• 发表时间: 2013
• 作者: Pathirana IN;Takahashi M;Hatoya S;Inaba T;Tamada H. Kawate N
• 通讯作者: Tamada H. Kawate N

PLASMA INSULIN-LIKE PEPTIDE 3 AND TESTOSTERONE CONCENTRATIONS IN MALE DOGS: HORMONAL DYNAMICS RELATED TO AGE AND CRYPTORCHIDISM

雄性犬血浆胰岛素样肽 3 和睾酮浓度：与年龄和隐睾症相关的荷尔蒙动态

• 发表时间: 2012
• 作者: Pathirana;I;Yamasaki;H;Takahashi;M;Tamada;H;Kawate;N.
• 通讯作者: N.