权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Determination of white clover saponins and agronomic factors affecting accumulation of saponins in white clover.

白三叶草皂苷的测定及影响白三叶草皂苷积累的农艺因素

基本信息

批准号：
04660283
负责人：
SUGAWARA Kazuo
金额：
$ 1.34万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04660283/
关键词：
White clover Ecotypes Saponins Ruminant bloat Glycosides Nitrogen fixation ブロート

项目摘要

(1).Analytical conditions of white clover saponins by TLC were investigated. It was determined that saponins contained in White clover were different from those in soybean.(2).Chromatogram and concentrations of the glycosides like saponin varied among white clover ecotypes, and the concentration showed seasonal variation, high from late May to July and low in autumn.(3).White clover cultivar was potted and cultivated with treatments of three nitrogen levels, N : 0,0.13,0.39g/ pot, and three shading levels, RLI : 100,60,40%.*As shading was taken at higher intensity, the regrowth length of top increased but both top and root weight decreased. On the growth of white clover, nitrogen level did not showed any effect.*Nitrogenase activity was the highest in the plant under N : 0g + RLI : 100% and decreased by nitrogen application and shading.*Eight bands of glycosides like saponins, (1) - (8), were identified by TLC and HPLC in white clover. The concentrations of band (3) and (4) were higher in N : 0 and those of band (1), (4) and (6) in RLI : 100%.(4).As the result of feeding experiment by sheep, a palatability of white clover was significantly high at the early stage of feeding, but it decreased with the progress of the time. It was supposed that white clover might contain inhibitory substances to eating. Ruminal contents bubbled markedly in sheep feeded white clover.(5).Utilization of fertilizer nitrogen was high in white clover ecotypes which the inhibition of nitrogenase activity by nitrogen application was small.

(1).考察了白三叶皂苷的薄层色谱分析条件。白三叶中皂苷的含量与大豆不同。(2)不同生态型白三叶中皂苷等皂苷的含量和色谱图不同，其含量呈季节性变化，5月下旬至7月间含量最高，秋季含量最低。(3)盆栽栽培白三叶品种，采用N：0，0.13，0.39g/盆，RLI：100，60，40%三个遮荫处理。氮素水平对白三叶的生长没有任何影响。*在N：0G+RLI：100%的条件下，植株的固氮酶活性最高，施氮和遮荫降低了固氮酶的活性。*经薄层色谱和高效液相色谱鉴定，白三叶中含有皂苷等8条糖苷(1)-(8)。(4)绵羊饲养试验结果表明，白三叶的适口性在饲喂初期显著升高，但随着饲养时间的延长，适口性逐渐降低。据推测，白三叶中可能含有抑制进食的物质。饲喂白三叶的绵羊瘤胃内容物显着起泡。(5)白三叶生态类型氮肥利用率高，施氮对固氮酶活性的抑制较小。