The effects of UVA irradiation on human neutrophil function

UVA照射对人体中性粒细胞功能的影响

• 批准号: 07407011
• 负责人: SUGAWARA Kazuo
• 金额: $ 10.11万
• 依托单位: HIROSAKI UNIVERSITY.
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07407011/
• 关键词: ultraviolet light; long-wave ultraviolet light; neutrophil; reactive oxygen species; serum opsonic activity; chemotactic activity; immunoglobulin; complement; プロテインキナーゼC; ルミノール; ルシゲニン; 血清オプソニン化活性; 活性酵素種; 化学発光

We investigated the in vitro effects of ultraviolet light A (UVA) irradiation on human neutrophil function. For measurements of reactive oxygen species (ROS) and serum opsonic activity, chemiluminescence method was used. Chemotactic activity was determined by Boyden technique. UVA irradiation at low dose of UVA (0.2-0.4 J/cm^2) did not affect the generation of OC1^-, whereas high dose of UVA (0.6-1.0 J/cm^2) suppressed its production by inactivating the signalling cascade which induce ROS on the membrane surface of neutrophils. No significant difference was observed in the chemotactic activity. The serum opsonic activity was increased by UVA irradiation. These results suggest that low dose of UVA does not impair the neutrophil-related immune system, and that higher dose of UVA is required for UVA phototherapy.

我们研究了紫外线A（UVA）照射对体外人中性粒细胞功能的影响。采用化学发光法测定活性氧和血清调理素活性。Boyden法测定趋化活性。低剂量UVA（0.2-0.4 J/cm ^2）照射不影响OC 1 ^-的产生，而高剂量UVA（0.6-1.0 J/cm ^2）通过灭活在中性粒细胞膜表面诱导ROS的信号级联反应来抑制OC 1 ^-的产生。在趋化活性方面没有观察到显著差异。UVA照射可使血清调理素活性升高。这些结果表明，低剂量的UVA不会损害嗜中性粒细胞相关的免疫系统，UVA光疗需要更高剂量的UVA。

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