Antioxidant activities of natural 9-cis β-carotene of Donliella bardawil on human neutrophil ROS production

Donliella bardawil 天然 9-cis β-胡萝卜素对人中性粒细胞 ROS 产生的抗氧化活性

• 批准号: 11357004
• 负责人: SUGAWARA Kazuo
• 金额: $ 9.91万
• 依托单位: Hirosaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11357004/
• 关键词: β -caroten; Donaliella bardawil; neutrophil; reactive oxygen species; chemiluminescence; antioxidant; 窒素酸化物

Much attention has been focused on the biological activity of geometrical isomers of β -carotene. In the present study, the in vitro antioxidant activities of a natural 9-cisβ-carotene purified from Donaliella bardawil and synthetic all-transβ -carotene were studied using enhanced human neutrophil chemiluminescence upon stimulation with opsonized zymosan. Lucigenin-dependent chemiluminescence was used for largely measuring superoxide anion (O_2) production, luminal-dependent chemiluminescence with sodium azide (NaN_3) and horseradish peroxidase (HRP) was used for measuring hydrogen peroxide (H_2O_2) production, and luminal-dependent chemiluminescence without oxidant metabolism modulator was used for determination of myeloperoxydase (MPO)-mediated production. With β -carotene concentration of 1.4 μM, neutrophils showed a decrease in lucigenin-dependent chemiluminescence to 75 % and 78 % of controls (P<0.05) by natural 9-cis β -carotene and synthetic all-trans β-carotene, respectively. With β-carotene concentration of 1.86 μM, neutrophils also showed a decrease in luminol-dependent chemiluminescence in the presence of NaN_3 and HRP to 86 % and 81 % of controls (P< 0.05) by natural 9-cis β -carotene and synthetic all-trans β-carotene, respectively. On the other hand, neutrophils did not show a statistically significant difference in any chemiluminescence assay by the same concentration of natural 9-cis β -carotene and synthetic a all-trans B -carotene. These results suggest that, up to the concentration of 1.86μM, natural 9-cis B -carotene and synthetic all-trans β -carotene exert equal antioxidant activities assessed by enhanced human neutrophil chemiluminescence.

β-胡萝卜素几何异构体的生物活性引起了人们的广泛关注。在本研究中，利用调理酵母聚糖刺激后增强的人中性粒细胞化学发光，研究了从Donaliella bardawil中纯化的天然9-顺式β-胡萝卜素和合成的全反式β-胡萝卜素的体外抗氧化活性。光泽精依赖性化学发光主要用于测量超氧阴离子（O_2）的产生，使用叠氮化钠（NaN_3）和辣根过氧化物酶（HRP）的管腔依赖性化学发光用于测量过氧化氢（H_2O_2）的产生，而没有氧化剂代谢调节剂的管腔依赖性化学发光用于测定 髓过氧化物酶（MPO）介导的生产。当β-胡萝卜素浓度为1.4 μM时，天然9-顺式β-胡萝卜素和合成全反式β-胡萝卜素使中性粒细胞的光泽精依赖性化学发光分别降低至对照的75%和78%（P<0.05）。当 β-胡萝卜素浓度为 1.86 μM 时，天然 9-顺式 β-胡萝卜素和合成全反式 β-胡萝卜素使中性粒细胞在 NaN_3 和 HRP 存在下鲁米诺依赖性化学发光分别降低至对照的 86% 和 81% (P< 0.05)。另一方面，在相同浓度的天然9-顺式β-胡萝卜素和合成的全反式β-胡萝卜素的任何化学发光测定中，中性粒细胞没有显示出统计学上的显着差异。这些结果表明，在浓度高达 1.86μM 时，天然 9-顺式 B-胡萝卜素和合成的全反式 β-胡萝卜素通过增强的人中性粒细胞化学发光评估具有相同的抗氧化活性。

项目成果

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Qiang Liu Q, Tadashi Shimoyama, Katsuhiko Suzuki, Takashi Umeda, Shigeyuki Nakaji, Kazuo Sugawara: "Effect of sodium butyrate on reactive oxygen species generation by human neutrophils"Scand. J. Gastroenterol. 36. 744-750 (2001)

Qiang Liu Q、Tadashi Shimoyama、Katsuhiko Suzuki、Takashi Umeda、Shigeyuki Nakaji、Kazuo Sukawara：“丁酸钠对人类中性粒细胞产生活性氧的影响”Scand。

Katsuhiko Suzuki, Mutsuo Yamada, Shigeyoshi Kurakake, Noriyoshi Okamura, Kanemitsu Yamaya, Qiang Liu, Satoru Kudoli, Kenji Kowatari, Shigeyuki Nakaji, Kazuo Sugawara: "Circulating cytokines and hormones with immunosuppressive but neutrophil-priming potent

Katsuhiko Suzuki、Mutsuo Yamada、Shigeyoshi Kurakake、Noriyoshi Okamura、Kanemitsu Yamaya、Qiang Liu、Satoru Kudoli、Kenji Kowatari、Shigeyuki Nakaji、Kazuo Sukawara：“具有免疫抑制作用但中性粒细胞启动作用的循环细胞因子和激素

Takuya Koie, Katsuhiko Suzuki, Tadashi Shimoyama, Takashi Umeda, Shigeyuki Nak aji, Kazuo Sugwara: "Effect of interferon-α on production of reactive oxygen species by human neutrophils"Luminescence. 16. 39-43 (2000)

Takuy​​a Koie、Katsuhiko Suzuki、Tadashi Shimoyama、Takashi Umeda、Shigeyuki Nak aji、Kazuo Sugwara：“干扰素-α 对人类中性粒细胞产生活性氧的影响”16. 39-43 (2000)。