MECHANISMS APPROACH TO GaAlAs DIODE LASER EFFECTS ON PRODUCTION OF REACTIVE OXYGEN SPECIES FROM HUMAN NEUTROPHILS AS A MODEL FOR THERAPEUTIC MODALITY AT CELLULAR LEVEL

GaAlAs 二极管激光对人类中性粒细胞活性氧产生影响的机制方法作为细胞水平治疗方式的模型

基本信息

  • 批准号:
    03454200
  • 负责人:
  • 金额:
    $ 2.43万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
  • 财政年份:
    1991
  • 资助国家:
    日本
  • 起止时间:
    1991 至 1993
  • 项目状态:
    已结题

项目摘要

There have been many reports on the applications of low reactive level laser(LLL) therapy for pain attenuation of pain removal. Our group has reported previously on the effects of in vitro irradiation of LLL particularly on the phagocytic activity of human neutrophils, using luminol-dependent chemiluminescence(LmCL) for measurement of reactive oxygen species(ROS) production from human neutrophils. But the mechanisms of the attenuation of phagocytic activity of neutrophils by LLL irradiation is not yet full understood.In this study, we used luminol-dependent and lucigenin-dependent chemiluminescence(LgCL) for detection of affected ROS producing process of human neutrophils by LLL irradiation. Two soluble action stimuli, N-formyl-Met-Leu-Phe(fMLP) and phorbol myristate acetate(PMA) were used to avoid the possible influence of lag-time from recognition to uptake of particles at the ROS production.In case of using fMLP as a stimulus, the maximum luminescence intensity of LmCL was increased but LgCL luminescence was decreased by LLL irradiation. When PMA was used as a stimulus, the times to reach the maximum luminescence intensity of LmCL and LgCL were shortend by LLL irradiation but there was no effect on the maximum luminescence intensity of both.These results suggest that LLL irradiation enhances the ROS production activity of human neutrophils by the activation of the superoxide converting system, the active element in which is mainly myeloperoxidase. LLL irradiation enable a more rapid activation of the superoxide production system, NADPH-oxidase.
低反应水平激光(LLL)治疗疼痛减轻或消除疼痛的应用已有许多报道。我们的小组以前曾报道在体外照射LLL的影响,特别是对人类中性粒细胞的吞噬活性,使用鲁米诺依赖的化学发光(LmCL)的测量活性氧(ROS)的生产从人类中性粒细胞。本研究采用鲁米诺和光泽精依赖的化学发光法(LgCL)检测了LLL照射对人中性粒细胞产生活性氧(ROS)过程的影响。采用两种可溶性作用刺激物N-甲酰-蛋氨酸-亮氨酸-苯丙氨酸(fMLP)和佛波醇肉豆蔻酸酯乙酸酯(PMA),以避免活性氧产生过程中颗粒从识别到摄取的滞后时间的影响,结果表明,在以fMLP为刺激物的情况下,LmCL的最大发光强度增加,而LgCL的发光强度降低。以PMA为刺激物时,LmCL和LgCL达到最大发光强度的时间被LLL照射缩短,但对两者的最大发光强度无影响,表明LLL照射通过激活以髓过氧化物酶为主的超氧化物转换系统而增强人中性粒细胞产生ROS的活性。LLL照射能够更快地激活超氧化物生产系统,NADPH氧化酶。

项目成果

期刊论文数量(38)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Makoto Yamaya: "Mechanistic approach to GaAlAs diode laser effects on production of reactive oxygen species from human neutrophils as a model for therapeutic modality at cellular level" Laser Therapy. 5. 111-116 (1993)
Makoto Yamaya:“GaAlAs 二极管激光影响人类中性粒细胞产生活性氧的机制方法,作为细胞水平治疗方式的模型”激光疗法。
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    0
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Emiko Kudoh: "Functional analysis of neutrophils from cord blood and maternal blood" Japanese Journal of Pediatric Hematology. 6. 415-422 (1992)
Emiko Kudoh:“脐带血和母血中的中性粒细胞的功能分析”,日本儿科血液学杂志。
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    0
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小野芳男: "Luminol-dependent Chemiluminescenceを指標とした好中球活性に及ぼす金属元素の影響" 弘前医学. 45(2). 198-207 (1993)
Yoshio Ono:“使用鲁米诺依赖性化学发光作为指示剂的金属元素对中性粒细胞活性的影响”Hirosaki Igaku 45(2) (1993)。
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    0
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工藤恵美子: "ヒト微量血からの新しい好中球分離法の検討" 日本衛生学雑誌. 47(2). 650-657 (1992)
Emiko Kudo:“从少量人体血液中分离中性粒细胞的新方法的研究”,日本卫生杂志 47(2) 650-657 (1992)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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管原 和夫: "Luminol-dependent chemilumine scenceによる好中球産生活性酸素種の測定" 弘前医学. 43. 328-337 (1992)
Kazuo Kanhara:“通过鲁米诺依赖性化学发光法测量中性粒细胞产生的活性氧”Hirosaki Igaku,43. 328-337 (1992)。
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    0
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SUGAWARA Kazuo其他文献

SUGAWARA Kazuo的其他文献

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{{ truncateString('SUGAWARA Kazuo', 18)}}的其他基金

Community Structure analysis of arbuscular mycorrhizalfungi in grassland ecosystem using DNA analysis
基于DNA分析的草原生态系统丛枝菌根真菌群落结构分析
  • 批准号:
    14360157
  • 财政年份:
    2002
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Antioxidant activities of natural 9-cis β-carotene of Donliella bardawil on human neutrophil ROS production
Donliella bardawil 天然 9-cis β-胡萝卜素对人中性粒细胞 ROS 产生的抗氧化活性
  • 批准号:
    11357004
  • 财政年份:
    1999
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Analyses of stress responses and thier adaptation mechanisms of the body in special references to formation and removal systems of reactive oxigen species.
分析身体的应激反应及其适应机制,特别参考活性氧的形成和去除系统。
  • 批准号:
    10307009
  • 财政年份:
    1998
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A).
Nitrogen flow in the legume forage plants-ruminant production system.
豆科饲料植物-反刍动物生产系统中的氮流。
  • 批准号:
    08660325
  • 财政年份:
    1996
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The effects of UVA irradiation on human neutrophil function
UVA照射对人体中性粒细胞功能的影响
  • 批准号:
    07407011
  • 财政年份:
    1995
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Investigation and Development of Environment Conservative and Animal Welfaristic Dairy Farming System
环保动物福利型奶牛养殖体系研究与发展
  • 批准号:
    07556145
  • 财政年份:
    1995
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Estimation of nutrients in diets from the values of carbon, nitrogen and hydrogen in diet and feces measured by CHN corder
根据 CHN cord 测量的饮食和粪便中的碳、氮和氢值估算饮食中的营养成分
  • 批准号:
    04557030
  • 财政年份:
    1992
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B)
Determination of white clover saponins and agronomic factors affecting accumulation of saponins in white clover.
白三叶草皂苷的测定及影响白三叶草皂苷积累的农艺因素
  • 批准号:
    04660283
  • 财政年份:
    1992
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Investigation of white clover ecotypes showing high nitrogenase activity under an abundant nitrogen and applying them to mixed grassland.
富氮条件下高固氮酶活性白三叶草生态型的调查及其在混合草地上的应用
  • 批准号:
    63560251
  • 财政年份:
    1988
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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Molecular mechanisms regulating astrocyte phagocytic activity
调节星形胶质细胞吞噬活性的分子机制
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    63570170
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INTERACTION OF COXIELLA BURNETI TO CELLS WITH LOW PHAGOCYTIC ACTIVITY
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