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MORPHOLOGICAL ANALYSIS OF SPECIFIC ULTRASTRUCTURES OF THE CELL MEMBRANE FORMED IN CELL-TO-CELL CONTACT ASSOCIATED WITH IMMUNOLOGICAL REACTION

与免疫反应相关的细胞间接触中形成的细胞膜的特定超微结构的形态学分析

基本信息

批准号：
04670024
负责人：
SASAKI Katsunori
金额：
$ 1.41万
依托单位：
Yamagata University (1994)Yokohama City University (1992-1993)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

项目摘要

The project as to ultrastructural analysis of the interaction between vascular endothelial cells and leukocytes in immunological reaction, which had been carried out from 1992 to 1994, has produced the following results.In orthotopic liver transplantation and lymph node transplantation, leukocytes interacted with endothelial cells actively. It was folloewed by formation of specific ultrastructures in both cells, e.g.filamentous bridges and high density of the cell membrane. Endothelial cells, which were associated with these phenomena, are characterized by well developed Golgi apparatus, which leads to the idea that glycoproteins play an important role in this interacion.To detect glycoproteins, e.g.adhesion molecules immunohistochemically and three-dimensionally, a new method is developed here. One feature of this method was that immunoreaction for EM (electron microscopy) preparation is finished in UW solution, organ preserving solution, before fixation. After the reaction, usual treatment for EM is possible and has guaranteed simultanous comparison between transmission EM and scanning EM and good preservation of ultrastrucure as well.The method disclosed that intercellular adhesion molecule (ICAM-1) expressed exclusively in processes and folds of high endothelial venule (HEV) cells. These structures combined with ICAM-1 functioned to trap lymphocytes, because lymphocytes often dropped into the furrows between folds and adhered to them.Finally as one conclusion from this project, though cell-to-cell interaction in in vivo immunological reaction is accomplished through interaction of adhesion molecules, ultarastructural changes of the cell membrane promate this interaction. To understand dynamism of cell-to-cell interaction, it is necessary to know not only whether molecules express or not, but also where they express in one cell three-dimensionally.

1992~1994年对免疫反应中血管内皮细胞与白细胞相互作用的超微结构进行了研究，取得了以下结果：在原位肝移植和淋巴结移植中，白细胞与内皮细胞相互作用活跃。随后在两个细胞中形成特殊的超微结构，如丝状桥和高密度的细胞膜。与这些现象有关的内皮细胞具有发达的高尔基体的特征，这导致了糖蛋白在这种相互作用中发挥重要作用的想法。为了用免疫组织化学和三维方法检测糖蛋白，如黏附分子，本文发展了一种新的方法。这种方法的一个特点是在固定前在器官保存液UW液中完成EM(电子显微镜)制备的免疫反应。反应结束后，常规治疗EM是可能的，并保证了透射EM和扫描EM的同时比较和超微结构的良好保存。该方法揭示了细胞间黏附分子(ICAM-1)仅在高内皮微静脉(HEV)细胞的突起和折叠中表达。这些结构和ICAM-1结合在一起起到了捕获淋巴细胞的作用，因为淋巴细胞经常掉进皱褶之间的沟里并附着在皱褶上。最后，本项目的一个结论是，尽管体内的细胞与细胞之间的相互作用是通过黏附分子的相互作用完成的，但细胞膜的超微结构变化促进了这种相互作用。为了了解细胞间相互作用的动力学，不仅需要知道分子是否表达，而且需要知道它们在一个细胞中的三维表达。