CELL BIOLOGICAL ANALYSIS OF ADHESIVE MECHANISM OF PERITONEUM AND PLEURA

腹膜与胸膜粘附机制的细胞生物学分析

• 批准号: 12670011
• 负责人: SASAKI Katsunori
• 金额: $ 2.18万
• 依托单位: SHINSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670011/
• 关键词: CELLULAR ADHESION MOLECULES; PERITOPNEUM; PLEURA; IMMUNO SEM; 大網; 肺胞; 接着分子; 免疫リポソーム; マウス

The failure of bio-defence of the peritoneal and pleural cavity, which is closely associated with cellular adhesion molecules, causes fatal damage to the body such as sepsis, but even if healing, severe adhesion of the serous membranes occurs inevitably and leads to intestinal obstruction or inhibition of the respiratory movement. In this project, the mechanism of the bio-defence and inhibition of adhesion in both cavities are analyzed with cell biological methods and discussed.1. Mechanism of the bio-defence of the peritoneal and pleural cavities. Interactions between ICAM-1 and LFA-1 & Mac-1 in normal, between VCAM-1 and VLA-4 in addition to ICAM-1 and LFA-1 in moderate inflammation and between fibronectin and VLA-4 in addition to VCAM-1 and VLA-4 in severe inflammation work according to each inflammatory stage. The first interaction between ICAM-1 and LFA-1 & Mac-1 promotes light adhesion, is done by microvilli of the mesothelial cells and microvilli or ruffles of leukocytes and pro … More bably used for migration. The second interaction between VCAM-1 and VLA-4 is necessary for fixation of leukocytes, which causes local secretion of cytokines. The last interaction brings about pathological adhesion through fibrous networks due to fibronectin.2. Source of leukocytes. In the peritoneal cavity, milky spots are well known as the supplying site for leukocytes. The area is always activated. Expression of ICAM-1 is four times higher than other areas. Leukocytes proliferate at that region. In the pleural cavity, the connective tissue near the costae including vascular network and adipocytes is identical to milky spots. The mesothelial cells at that region proliferate markedly, which suggest they are stem cells.3. Inhibition of migration and adhesion. In the peritoneal cavity, ICAM-1, VCAM-1, VLA-4, LFA-1,Mac-1 are blocked with antibodies, which cause drastic inhibition of leukocyte migration. From this result and previous observations, inhibition of VCAM-1 and VLA-4 may be proposed to suppress adhesion of serous membranes. Less

与细胞粘附分子密切相关的腹膜和胸膜腔的生物防御的失败对身体造成致命的损害，例如脓毒症，但即使愈合，也不可避免地发生浆膜的严重粘附，并导致肠梗阻或呼吸运动的抑制。本课题采用细胞生物学方法，分析和探讨了两种腔体的生物防御和粘附抑制机制.腹膜和胸膜腔的生物防御机制。在正常情况下ICAM-1与LFA-1和Mac-1之间的相互作用，在中度炎症中VCAM-1与VLA-4之间以及ICAM-1和LFA-1之间的相互作用，以及在重度炎症中纤连蛋白与VLA-4之间以及VCAM-1和VLA-4之间的相互作用根据每个炎症阶段起作用。ICAM-1与LFA-1和Mac-1之间的第一种相互作用促进光粘附，通过间皮细胞的微绒毛和白细胞的微绒毛或皱褶以及细胞粘附分子的粘附来完成。 ...更多信息 可用于迁移。VCAM-1和VLA-4之间的第二种相互作用对于白细胞的固定是必需的，这导致细胞因子的局部分泌。最后一种相互作用通过纤维连接蛋白引起的纤维网络引起病理性粘连。白细胞来源。在腹膜腔中，乳斑是众所周知的白细胞供应部位。该区域始终处于激活状态。ICAM-1的表达是其他区域的四倍。白细胞在该区域增殖。胸膜腔内靠近肋骨的结缔组织包括血管网和脂肪细胞，与乳斑一致。该区域的间皮细胞增殖明显，提示其为干细胞.抑制迁移和粘附。在腹膜腔中，ICAM-1、VCAM-1、VLA-4、LFA-1、Mac-1被抗体阻断，这导致白细胞迁移的剧烈抑制。根据该结果和先前的观察，可以提出抑制VCAM-1和VLA-4以抑制浆膜的粘附。少

项目成果

Sasaki K, Johkura K, Ogiwara N, Liang Y, Cui L, Teng R, Okouchi Y, Asanuma K, Ishida O, Maruyama K: "Three-dimensional morphological analysis of antigen-antibody reaction in hepatic sinusoids preserved in hypothermic UW solution"Cryobiology. 42. 145-150 (

Sasaki K、Johkura K、Ogiwara N、Liang Y、Cui L、Teng R、Okouchi Y、Asanuma K、Ishida O、Maruyama K：“低温 UW 溶液中保存的肝窦中抗原抗体反应的三维形态学分析”

Liang Y, Sasaki K: "Expression of adhesion molecules relevant to leukocyte migration on the microvilli of liver peritoneal mesothelial cells."Anat Rec. 258. 39-46 (2000)

梁Y，佐佐木K：“与白细胞迁移相关的粘附分子在肝腹膜间皮细胞微绒毛上的表达。”Anat Rec。

Johkura K, Liang Y, Teng R, Ogiwara N, Sasaki K: "Nephrogenesis accopanied by vascularization in the mouse embryonic metanephros transplanted into the adult kidney for the creation of additional nephros"Nephrology. 7. 86-94 (2002)

Johkura K、Liang Y、Teng R、Ogiwara N、Sasaki K：“移植到成人肾脏中的小鼠胚胎后肾中伴随血管化的肾发生，用于产生额外的肾”肾脏病学。

Johkura K, Liang Y, Teng R, Ogiwara N, Sasaki K.: "Nephrogenesis accopanied by vascularization in the mouse embryonic metanephros transplanted into the adult kidney for the creation of additional nephros."Nephrology. 7. 86-94 (2002)

Johkura K、Liang Y、Teng R、Ogiwara N、Sasaki K.：“移植到成人肾脏中的小鼠胚胎后肾中伴随着血管化的肾发生，用于产生额外的肾。”肾脏病学。

Liang Y, Johkura K, Ogiwara N, Sasaki K: "Expression of adhesion molecules and fibronectin of activated peritoneal surface with Lipopolysaccharide(LPS) analyzed with ImmunoSEM."Ann Anat. 183. 353-356 (2001)

Liang Y、Johkura K、Ogiwara N、Sasaki K：“用免疫扫描电镜分析脂多糖 (LPS) 激活腹膜表面粘附分子和纤连蛋白的表达。”Ann Anat。