THE MORPHOLOGICAL ANALYSIS OF THE PERITONEUM IN DEFENCE SYSTEM
防御系统腹膜的形态分析
基本信息
- 批准号:07670002
- 负责人:
- 金额:$ 1.41万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1995
- 资助国家:日本
- 起止时间:1995 至 1997
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
To establish the peritoneum as defense organ, interaction between the peritoneal mesothelial cells and immuneassociated cells were analyzed morphologically through expression of cellular adhesion molecules with immunoSEM.ICAM-1 (intercellular adhesion molecule-1) is an important molecule of IgG superfamily associated with leukocyte migration through stick adhesion. This molecule was selectively expressed on the microvilli of the peritoneum and was upregulated by LPS (Lipopolysaccharide). The peak was 24 hrs after stimulation. ICAM-1 was suggested to work in both normal and inflammatory conditions.In the experimental peritonitis, a large number of macrophages appeared on the peritoneum. They had LFA-1 (leukocyte function-associated antigen 1) and Mac-1, which are ligands of ICAM-1, on the cell surface. Mac-1 was restricted to the microvilli of the macrophages in activated condition. The microvilli of the peritoneum expressed ICAM-1 moderately. It was confirmed that these abhesions was necessary for iinteraction between the peritoneum and leukocytes.The peritoneum expressed VCAM-1 in addition to ICAM-1 after LPS stimulation. Other adhesions (ICAM-2, ELAM-1, PECAM-1, CD105) were not detected. VCAM-1 was restricted to microvilli as well as ICAM-1. After both expression, s leukocytes fixed to the peritoneum Double immunostaining showed localizations of ICAM-1 and VCAM-1 were near to each other.These results showed that (1) Microvilli with ICAM-1 is a road for leukocyte migration in the normal condition and (2) in inflammation, ICAM-1 and VCAM-1 cooperate to fix leukocytes to the peritoneum and the peritoneum works as defense organ through interaction of ICAM-1 and VCAM-1 with LFA-1, Mac-1 and VLA-4.
为了将腹膜作为防御器官,通过免疫扫描电镜观察腹膜间皮细胞与免疫相关细胞之间的相互作用。ICAM-1(细胞间黏附分子-1)是一种重要的免疫球蛋白G超家族分子,参与了白细胞的粘附性迁移。该分子选择性地表达在腹膜微绒毛上,并被脂多糖(LPS)上调。峰值出现在刺激后24小时。ICAM-1被认为在正常和炎症条件下都有作用。在实验性腹膜炎中,腹膜出现大量巨噬细胞。它们的细胞表面有LFA-1(白细胞功能相关抗原1)和Mac-1,这是ICAM-1的配体。在激活状态下,Mac-1仅局限于巨噬细胞的微绒毛。腹膜微绒毛中度表达ICAM-1。证实这些粘连是腹膜与白细胞相互作用所必需的。在脂多糖刺激后,腹膜除表达ICAM-1外,还表达VCAM-1。未检测到其他粘连(ICAM-2、ELAM-1、PECAM-1、CD105)。VCAM-1定位于微绒毛和ICAM-1。细胞间黏附分子-1和血管细胞黏附分子-1在微绒毛中的定位接近,说明微绒毛在正常情况下是白细胞迁移的途径,在炎症状态下,细胞间黏附分子-1和血管细胞黏附分子-1协同作用将白细胞固定在腹膜上,腹膜通过细胞间黏附分子-1和血管细胞黏附分子-1与LFA-1,Mac-1和VLA-4的相互作用发挥防御器官的作用。
项目成果
期刊论文数量(24)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Yamaguchi, K.et al.: "Distribution of intercellular adhesion molecule-1 on lleukocytes and corneal endothelium after endotoxin stimulation in rats." Int.Opthalmol.19. 303-306 (1996)
Yamaguchi, K.et al.:“大鼠内毒素刺激后细胞间粘附分子 1 在白细胞和角膜内皮上的分布。”
- DOI:
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- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Sasaki,K. et al.: "Three-dimensional distribution of intercellular adhesion molecule-1(ICAM-1) on lymphocytes in the HEV analyzed by backscatter electron(BSE) imaging." Acta Anat.(revised).
佐佐木,K.
- DOI:
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- 影响因子:0
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Yamagushi, K.et al.: "Early expression of intercellular adhesion molecule-1 in the corneal endothelium stimulated by endotoxin : An immuno-scanning electron microscopic analysis." Jpn.J.Opthalmol.40. 12-17 (1996)
Yamagushi, K. 等人:“内毒素刺激的角膜内皮细胞间粘附分子 1 的早期表达:免疫扫描电子显微镜分析。”
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- 影响因子:0
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Sasaki,K.et al.: "Ultrastructural localization of the intercellular adhesion molecule (ICAM-1) on the cell surface of high endothelial venules in lymph nodes." Anat.Rec.244. 105-111 (1996)
Sasaki,K.et al.:“淋巴结高内皮微静脉细胞表面细胞间粘附分子 (ICAM-1) 的超微结构定位。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
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- 通讯作者:
Endo, H.et al.: "Three-dimensional and ultrastructural ICAM-1 distribution in the choroid plexus, arachnoid membrane and dural sinus of inflammatory rats induced by LPS injection." Brain Res.(in press.).
Endo, H.等人:“LPS 注射诱导的炎症大鼠脉络丛、蛛网膜和硬脑膜窦中 ICAM-1 的三维和超微结构分布。”
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SASAKI Katsunori其他文献
SASAKI Katsunori的其他文献
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$ 1.41万 - 项目类别:
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Nobel quantification system of non-transferrin-bound iron utilizing automated analyzer
使用自动分析仪的非转铁蛋白结合铁的 Nobel 定量系统
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23591363 - 财政年份:2011
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Development of the PKR-dependent replicating adenovirus
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18591433 - 财政年份:2006
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$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
CELL BIOLOGICAL ANALYSIS OF ADHESIVE MECHANISM OF PERITONEUM AND PLEURA
腹膜与胸膜粘附机制的细胞生物学分析
- 批准号:
12670011 - 财政年份:2000
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$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
STUDY AS TO INTERACTION BETWEEN IMMUNOLIPOSOME AND VASCULAR ENDOTHELIAL CELLS IN VIVO
体内免疫脂质体与血管内皮细胞相互作用的研究
- 批准号:
10670002 - 财政年份:1998
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
MORPHOLOGICAL ANALYSIS OF SPECIFIC ULTRASTRUCTURES OF THE CELL MEMBRANE FORMED IN CELL-TO-CELL CONTACT ASSOCIATED WITH IMMUNOLOGICAL REACTION
与免疫反应相关的细胞间接触中形成的细胞膜的特定超微结构的形态学分析
- 批准号:
04670024 - 财政年份:1992
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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