Nobel quantification system of non-transferrin-bound iron utilizing automated analyzer

铁过量会导致各种器官损伤。当转铁蛋白完全饱和时，血清中出现非转铁蛋白结合铁（NTBI）。由于NTBI与氧化应激有关，因此监测血清NTBI水平对铁超载患者至关重要。在本研究中，我们的目的是开发新的NTBI检测试剂相应的自动分析仪通过修改高效液相色谱（HPLC）为基础的NTBI测定（金标准法）。我们的系统和HPLC方法之间的NTBI值的相关系数为0.923，表明我们的系统的准确度与金标准方法相当。我们的新NTBI检测系统应该是一个强大的工具，研究生理和临床的重要性NTBI。

Iron overload causes various organ damages. When transferrin is fully saturated, non-transferrin-bound iron (NTBI) appears in the serum. Since NTBI relates to oxidant stress, monitoring serum NTBI levels is crucial for iron-overloaded patients. In the present study, we aimed to develop the novel NTBI measuring reagent corresponding to the automatic analyzer by modifying the high-performance liquid chromatography (HPLC)-based NTBI assay (the gold standard method). The correlation coefficient for NTBI values between our system and HPLC method was 0.923, indicating that the accuracy of our system is comparable to the gold standard method. Our novel NTBI assay system should be a powerful tool for studying physiological and clinical importance of NTBI.