Nobel quantification system of non-transferrin-bound iron utilizing automated analyzer

铁超载会导致各种器官损伤。当转铁蛋白完全饱和时，血清中会出现非转铁蛋白结合铁(NTBI)。由于NTBI与氧化应激有关，因此监测血清NTBI水平对铁负荷过高的患者至关重要。在本研究中，我们旨在通过对基于高效液相色谱(HPLC)的NTBI测定方法(金标准方法)的改进，开发出与自动分析仪相对应的新型NTBI测定试剂。本系统与高效液相色谱法测定的NTBI值的相关系数为0.923，表明本系统的准确度与金标法相当。我们的新的NTBI检测系统将为研究NTBI的生理和临床意义提供有力的工具。

Iron overload causes various organ damages. When transferrin is fully saturated, non-transferrin-bound iron (NTBI) appears in the serum. Since NTBI relates to oxidant stress, monitoring serum NTBI levels is crucial for iron-overloaded patients. In the present study, we aimed to develop the novel NTBI measuring reagent corresponding to the automatic analyzer by modifying the high-performance liquid chromatography (HPLC)-based NTBI assay (the gold standard method). The correlation coefficient for NTBI values between our system and HPLC method was 0.923, indicating that the accuracy of our system is comparable to the gold standard method. Our novel NTBI assay system should be a powerful tool for studying physiological and clinical importance of NTBI.