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The correlated analysis between the morphological and electrophysiological characteristics of the projection neurons in the reeler cerebral cortex.

Reeler大脑皮层投射神经元形态与电生理特征的相关分析

基本信息

批准号：
04670031
负责人：
INOUE Yoshiro
金额：
$ 1.34万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

The plasticity of the signal transduction system intrinsically present in the projection neurons was investigated using the reeler cerebral and cerebellar cortex by means of the morphomeiric study, electrophysiological analysis by the slice-method and in situ hybridization method for NMDA receptor channel. The results obtained were as follows : (1)The corticospinal, corticothalamic and corticocommissural neurons of the reeler and normal mice were retrogradely labeled by biocytin and the entire shape of the labeled neurons were input into the personal computer system through the degitizer and the morphometric calculation was carried out. The morphological characteristics of the projection neurons in the normal and reeler was possibly determined by their own projection sites, whereas the extension pattern of the apical dendrites was influenced in their location within the cerebral columna and input factors from other neurons. (2)The electrophysiological equipment was prepared for the brain-slice methos. The techniques for slicing brain, pulling the electrical micropipettes and labelling neurons in the slice were established using the normal mice. (3)By in situ hybridization study for the NMDA receptor channel the Purkinje cell in the reeler cerebellum revealed anomalous expression with special reference to the topological distribution. This means the NMDA recetor channel possibly have an intrinsic variability influenced by the synaptic environment even in the cerebral cortex. (4)The reelet neurons in the cerebrum and cerebellum were highly useful for the investigation of the intrinsic factors for expressing transduction system within the nerve cells and plasticity and variability of the neuronal circuits.

采用形态学研究、切片法电生理分析和NMDA受体通道原位杂交方法，研究了大鼠大脑和小脑皮质投射神经元固有的信号转导系统的可塑性。结果如下：（1）用生物胞素逆行标记大鼠和正常小鼠的皮质脊髓、皮质丘脑和皮质连合神经元，并将标记神经元的整体形状通过数字化仪输入计算机系统进行形态计量学计算。正常组和卷轴组投射神经元的形态特征可能由其投射部位决定，而顶树突的延伸方式则受其在脑柱内的位置和其他神经元的输入因素的影响。（2）制作脑切片电生理装置。利用正常小鼠建立了脑切片、微量吸移管牵拉和脑片神经元标记技术。(3)By NMDA受体通道的原位杂交研究显示，在Reeler小脑中的浦肯野细胞的异常表达，特别是关于拓扑分布。这意味着即使在大脑皮层中，NMDA受体通道也可能具有受突触环境影响的内在变异性。（4）大脑和小脑中的网状神经元对于研究神经细胞内表达转导系统的内在因素以及神经回路的可塑性和可变性具有重要意义。