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The morphological analysis of oligodendrocytic behavior during development of the nerve tracts.

神经束发育过程中少突胶质细胞行为的形态学分析。

基本信息

批准号：
62570022
负责人：
INOUE Yoshiro
金额：
$ 1.28万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

The behavior of the oligodendrocytes during myelin formation with special reference to the development of the nerve tracts was investigated by using neurological mutants, mld (myelin deficient mouse) and shiverer, which are the autosomal recessive mutation. The results are as follows:1. By the Golgi silver stain the immature oligodendrocytes of both normal and mld possessed predominantly numerous cytoplasmic processes, several of which were associated closely with axons in the nerve tract (the pyramidal tract in this study). The inherited defect that myelin basic protein is absent in the central myelin was not related with the initial behavior that oligodendrocytic processes apply to the axons to enclose them.2. The myelin lamellae of mld are prinicipally consisted of tri-lamellar units without the major dense lines quitely resembling those of the shiverer. However, as for mld myelin lamellae, the major dense lines occurred sporadically and with different amount among different myelin … More sheaths. This means that the structure and chemical composition of the internodal segments of myelin lamellas are determined by the oligodendrocytes concerened.4. At 4-5 day after birth when the pyramidal tract could reach the target neurons in the spinal cord, as detected by diI (a supersensitive fluorescent dye ) injection into the motor cortex, the oligodendrocytes of the mld in the pyramidal tract predominant increased in number, resulting in the oligodedrocytic hyperplasia in the adult pyramidal tract, and in addition, raised the disintegration of the Golgi complex and rough-surfaced endoplasmic reticulum. This phenomenon reveals the oligodendrocytic reaction to the axons might be induced by establishment of the connection of axons with the target neurons. 5. From the findings of the myelin lamellae and oligodendrocytes of the frist hybrid mice produced by intercrossing homozygotes of shiverer and mld, these two mutations were allelic, and the morphology of the myelin lamellae were closely similar to those of the shiverer, whereas some aspects revealed the properties of those of mld. Less

通过使用神经学突变体mld（髓鞘缺陷小鼠）和shiverer（常染色体隐性突变）研究了髓鞘形成期间少突胶质细胞的行为，特别是神经束的发育。研究结果如下：1.高尔基体银染显示，正常和mld的未成熟少突胶质细胞均具有大量的胞质突起，其中一些突起与神经束（本研究中为锥体束）的轴突紧密相连。髓鞘碱性蛋白在中央髓鞘中缺失的遗传缺陷与少突胶质细胞突起应用于轴突以包围它们的初始行为无关. mld的髓鞘主要由三层单位组成，没有与shiverer相似的主要致密线。而MLD的髓鞘板层主要致密线呈散在分布，不同髓鞘之间密度不一 ...更多信息鞘。这意味着髓鞘板层节间节段的结构和化学组成是由少突胶质细胞决定的。在生后4-5天，用diI检测到锥体束可到达脊髓内的靶神经元（一种超敏感的荧光染料）注射到运动皮质中，锥体束中MLD的少突胶质细胞数量显著增加，导致成年锥体束中的少突胶质细胞增生，此外，提高高尔基复合体和粗糙面内质网的解体。这一现象揭示了少突胶质细胞对轴突的反应可能是通过轴突与靶神经元建立连接而引起的。5.从shiverer和mld纯合子杂交产生的第一代杂交小鼠的髓鞘和少突胶质细胞的观察结果来看，这两种突变是等位的，髓鞘的形态与shiverer非常相似，但某些方面显示了mld的特征。少