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ANALYSIS Of FUNCTIONAL MECHAMISM OF GLUTAMATE RECEPTORS IN NEURO-LOGICAL MUTANT MICE BY PHOTOMETRIC SYSTEM.

通过光度系统分析神经突变小鼠谷氨酸受体的功能机制。

基本信息

批准号：
07458198
负责人：
INOUE Yoshiro
金额：
$ 4.86万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

项目摘要

1 The diversed oxpression of NMDA receptor subunits (epsilon1, epsilon2, epsilon3, epsilon4 and zeta1) and delta2 subunit were elucidated in the mouse cerebellum during development. In addition, expressions of NMDA receptor subunits in the adult normal mice were different from those of the reeler and straggerer mutants in combination of subunits expressed. Thus expression of NMDA receptor subunits might be influenced by the extrinsic factors like the afferent input. The delta2 subunit tended to accumulate in the dendritic spines of the Purkinje cells closely related with synaptic formation of parallel fibers.2 The mice defective in glutamate receptor gene were generated. the epsilon1 mutant shows a moderate deficiency in spatical learning, significant reduction of the NMDA receptor channel current and LTP at the hippocumpus. The mutant mice defective in the epsilon1 subunit showed on suckling response and died shortly after birth. This disorder resulted in abnormal termination of the trigenimal afferent fibers. The epsilon4 mutant showed only a reduced spontaneous activity, bur not any morphological abnormality. By delta2 mutant this subunit is an important factor in strength and stability of synaptic connection between dendritic spines and parallel fibers.3 The electrophysiological system for electric stimulation, electrophoretic injection and putch-clump was set up partially by this gland, and were successfully used for fine labeling of the climbing fibers from the olivary nucleus. The optical imaging system (Deltaron 1700) was also set up and the reaction of the Purkinje cells under stimulation of the parallel fibers was recorded, but the usage for analysis of the glutamate receptor functions was remained as a future problem.

1. NMDA受体ε 1、ε 2、ε 3、ε 4和zeta 1亚基及δ 2亚基在小鼠小脑发育过程中表达的差异。此外，成年正常小鼠与reeler和straggerer突变体的NMDA受体亚基表达在亚基表达组合上存在差异。因此，NMDA受体亚单位的表达可能受到传入输入等外在因素的影响。delta 2亚单位倾向于聚集在与平行纤维突触形成密切相关的浦肯野细胞的树突棘上。2产生谷氨酸受体基因缺陷小鼠。ε 1突变体在空间学习中表现出中度缺陷，NMDA受体通道电流和海马LTP显著降低。ε 1亚基缺陷的突变小鼠表现出哺乳反应，并在出生后不久死亡。这种疾病导致三叉神经传入纤维的异常终止。ε 4突变体仅表现出减少的自发活动，但没有任何形态学异常。通过delta 2突变体，该亚基是树突棘和平行纤维之间突触连接的强度和稳定性的重要因素。3电刺激，电泳注射和putch-clump的电生理系统部分由该腺体建立，并成功地用于精细标记橄榄核的攀爬纤维。还建立了光学成像系统（Deltaron 1700），并记录了浦肯野细胞在平行纤维刺激下的反应，但用于谷氨酸受体功能的分析仍是未来的问题。