The human plasma glutathione peroxidase gene ; organization, nucleotide sequence and localization to chromosome 5q32.

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A genomic clone encoding the human plasma glutathione peroxidase (PGPx), amajor enzyme reducing lipid hydroperoxide and hydrogen peroxide in plasma, was isolated and 5618 nucleotides were determined. The nucleotide sequence data revealed that the PGPx gene is composed of five exons spanning approximately 10 kilobases. Primer extension experiments mapped the transcription start point at 298 nucleotides upstream from the predicted translation initiation codon. Seventeen nucleotides upstream from the polyadenylation site of the gene, an uncanonical polyadenylation signal, AGTAAA, was found. The human PGPx gene was localized on chromosome 5 band q32 by fluorescence in situ hybridization.