DEVELOPMENT OF SEROLOGICAL DIAGNOSIS OF BANCROFTIAN FILARIASIS BY A MONOCLONAL ANTIBODY

将班氏丝虫免疫小鼠的脾细胞与NS-1骨髓瘤细胞融合，获得了分泌抗微丝蚴可溶性部分或微丝蚴鞘表面抗体的杂交瘤细胞系。成功建立了14株克隆化抗体分泌细胞系。对其中两个克隆进行了表征。1个克隆（1G 10）分泌的IgM抗体具有高度的阶段特异性，可识别微丝蚴，但不与成虫反应。另一个抗微丝蚴鞘的克隆（2G 01）能产生IgG_1，但不与成虫结合。140例微丝蚴血症者中135例检出丝虫抗原，25例无微丝蚴血症者中1例检出丝虫抗原，30例地方病对照中5例检出丝虫抗原。在非流行区有多种寄生虫感染者血清中未检出抗原。寄生虫抗原滴度与夜间血涂片中微丝蚴计数显著相关。对23例班氏丝虫病患者血清中16例进行了抗班氏丝虫循环抗原抗体检测。尽管存在这种局限性，但通过酶免疫测定法检测寄生虫抗原提供了优于先前可用的用于诊断活动性班氏瓦氏体感染的方法的显著优势。

Hybridoma cell lines, which secreted antibodies directed against either the soluble fraction of microfilaria or surface of sheath of microfilaria, were produced by fusion of spleen cells of W.bancrofti microfilariae-immunized mice with NS-1 myeloma cells. Fourteen cloned antibody-secreting cell lines were successfully established. Two of these clones were characterized. One clone (1G10) secreted an IgM antibody that had a high stage specificity with recognizing the microfilaria but did not react against adult worm. Another clone (2G01) directed against the sheath of microfilaria was found to produce an IgG_1, but did not show a binding to adult filaria.The evaluation of monoclonal-based enzyme immunoassay for detecting soluble microfilarial antigen in sera collected in an endemic area of filariasis was carried out. Filarial antigen was detected in sera from 135 of 140 microfilaremic patients, 1 of 25 amicrofilaremic patients with clinical filariasis, and 5 of 30 endemic controls. Antigen was not detected in sera form nonendemic areas s who had a variety of other helminth infections. Parasite antigen titer were significantly correlated with microfilrial counts in night blood smears. Antibodies to circulating W.bancrofti antigen were detected 16 of 23 antigen-negative sera from patients with clinical filariasis. Despite this limitation, detecting parasite antigen by enzyme immunoassay provides significant advantages over previously available methods for diagnosing active W.bancrofti infection.