DEVELOPMENT OF SEROLOGICAL DIAGNOSIS OF BANCROFTIAN FILARIASIS BY A MONOCLONAL ANTIBODY

将免疫班氏丝虫小鼠的脾细胞与NS-1骨髓瘤细胞融合，获得分泌针对微丝虫可溶性部分或微丝虫鞘表面抗体的杂交瘤细胞系。成功地建立了14个克隆的抗体分泌细胞系。对其中两个克隆进行了鉴定。1个克隆(1G10)分泌的IgM抗体对微丝虫具有较高的阶段特异性，但对成虫不产生反应。另一株针对微丝虫鞘的克隆(2G01)可产生免疫球蛋白Ig_1，但不与成丝虫结合。在140例微丝虫病患者中135例检测到丝虫抗原，25例临床丝虫病患者中1例检测到丝虫抗原，30例地方性对照中检出5例。非流行区人群血清中未检出抗原S，他有多种其他蠕虫感染。寄生虫抗原滴度与夜间血涂片微丝计数显著相关。在23份抗原阴性的丝虫病患者血清中检出16份抗班氏丝虫循环抗原抗体。尽管存在这一局限性，但用酶免疫分析法检测寄生虫抗原在诊断活动性班氏假丝酵母菌感染方面具有明显的优势。

Hybridoma cell lines, which secreted antibodies directed against either the soluble fraction of microfilaria or surface of sheath of microfilaria, were produced by fusion of spleen cells of W.bancrofti microfilariae-immunized mice with NS-1 myeloma cells. Fourteen cloned antibody-secreting cell lines were successfully established. Two of these clones were characterized. One clone (1G10) secreted an IgM antibody that had a high stage specificity with recognizing the microfilaria but did not react against adult worm. Another clone (2G01) directed against the sheath of microfilaria was found to produce an IgG_1, but did not show a binding to adult filaria.The evaluation of monoclonal-based enzyme immunoassay for detecting soluble microfilarial antigen in sera collected in an endemic area of filariasis was carried out. Filarial antigen was detected in sera from 135 of 140 microfilaremic patients, 1 of 25 amicrofilaremic patients with clinical filariasis, and 5 of 30 endemic controls. Antigen was not detected in sera form nonendemic areas s who had a variety of other helminth infections. Parasite antigen titer were significantly correlated with microfilrial counts in night blood smears. Antibodies to circulating W.bancrofti antigen were detected 16 of 23 antigen-negative sera from patients with clinical filariasis. Despite this limitation, detecting parasite antigen by enzyme immunoassay provides significant advantages over previously available methods for diagnosing active W.bancrofti infection.