DEVELOPMENT OF SEROLOGICAL DIAGNOSIS OF BANCROFTIAN FILARIASIS BY A MONOCLONAL ANTIBODY

将微丝虫免疫小鼠的脾脏细胞与NS-1骨髓瘤细胞融合制备杂交瘤细胞株，该细胞株可分泌针对微丝虫可溶性部分或微丝虫鞘表面的抗体。成功建立了14株克隆抗体分泌细胞系。对其中两个克隆进行了鉴定。一个克隆（1G10）分泌一种IgM抗体，该抗体具有识别微丝虫的高阶段特异性，但对成虫没有反应。另一个克隆（2G01）直接作用于微丝虫丝鞘产生IgG_1，但不与成虫丝虫丝结合。对某丝虫病流行地区血清可溶性微丝抗原单克隆酶免疫分析法进行了评价。140例微丝虫病患者中有135例、25例临床丝虫病非微丝虫病患者中有1例、30例地方性对照中有5例血清检测到丝虫病抗原。在有多种其他寄生虫感染的非流行地区的血清中未检测到抗原。夜血涂片中寄生虫抗原滴度与微丝计数显著相关。23例临床丝虫病患者血清中16例检测到循环白细胞抗原抗体。尽管存在这种局限性，但与以前可用的诊断活动性班氏梭菌感染的方法相比，用酶免疫分析法检测寄生虫抗原具有显著的优势。

Hybridoma cell lines, which secreted antibodies directed against either the soluble fraction of microfilaria or surface of sheath of microfilaria, were produced by fusion of spleen cells of W.bancrofti microfilariae-immunized mice with NS-1 myeloma cells. Fourteen cloned antibody-secreting cell lines were successfully established. Two of these clones were characterized. One clone (1G10) secreted an IgM antibody that had a high stage specificity with recognizing the microfilaria but did not react against adult worm. Another clone (2G01) directed against the sheath of microfilaria was found to produce an IgG_1, but did not show a binding to adult filaria.The evaluation of monoclonal-based enzyme immunoassay for detecting soluble microfilarial antigen in sera collected in an endemic area of filariasis was carried out. Filarial antigen was detected in sera from 135 of 140 microfilaremic patients, 1 of 25 amicrofilaremic patients with clinical filariasis, and 5 of 30 endemic controls. Antigen was not detected in sera form nonendemic areas s who had a variety of other helminth infections. Parasite antigen titer were significantly correlated with microfilrial counts in night blood smears. Antibodies to circulating W.bancrofti antigen were detected 16 of 23 antigen-negative sera from patients with clinical filariasis. Despite this limitation, detecting parasite antigen by enzyme immunoassay provides significant advantages over previously available methods for diagnosing active W.bancrofti infection.