Development research into molecular target diagnosis and treatment method of cancer of oral cavity that makes Wnt signaling factor target

以Wnt信号因子为靶点的口腔癌分子靶向诊疗方法的开发研究

• 批准号: 18592183
• 负责人: KOBAYASHI Masashi
• 金额: $ 2.53万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18592183/
• 关键词: cancer of oral cavity; diagnosis; treatment; Wnt signaling; β-catenin

It is thought that the cancer of oral cavity is still feared as a fatal disease ease regardless of remarkable advancement of the diagnosis and the treatment method of the cancer of oral cavity and a big obstacle is given from the change to a morbid state of the face it to QOL. Little research of the relation between the cancer of oral cavity and the Wnt cell signaling route is advanced though β-Catenin is thought to be one of the oncogenes. It was thought that it was necessary to promote this research in the emergency for overcoming at the early stage of the cancer of oral cavity, examined as follows, and it reaped the benefit.1. Examination of irregular accumulation to cytoplasm and nucleus of B-Catenin of culture mouth cancer cell.Both what β-Catenin has accumulated abnormally in the cytoplasm and the nucleus and the thing that had not accumulated the label by the fluorescent antibody to recognize β-Catenin of the culture flatness cuticle cancer cell and the culture saliva glandular carcinoma cell, the observation with the electron microscope, and the retrieval whether β-Catenin had accumulated abnormally in the cytoplasm and the nucleus existed.2. Examination of gene mutation of β-Catenin of culture mouth cancer cell. RNA of β-Catenin of the culture flatness cuticle cancer cell and the culture saliva glandular carcinoma cell was extracted, the presence of the gene mutation was retrieved by the PCR-SSCP method, direct sequence method Denco mutation was tried in addition, and the decision of the position was tried. In the culture mouth cancer cell that had abnormally accumulated β-Catenin in the cytoplasm and the nucleus, the one with the gene mutation of β-Catenin and the one that not was existed. Moreover, the area was a part phosphorylated by GSK3.

人们认为，尽管口腔癌的诊断和治疗方法有了显著的进步，但口腔癌仍然是一种致命的疾病，并且从改变到病态到QOL是一个很大的障碍。虽然β-Catenin被认为是口腔癌的癌基因之一，但Wnt细胞信号通路与口腔癌的关系研究甚少。有人认为，这是必要的，以克服在口腔癌的早期阶段的紧急情况下，推进这项研究，检查如下，它获得了好处.培养口腔癌细胞β-连环蛋白向胞浆和胞核不规则聚集的检测：用荧光抗体标记培养扁平角质层癌细胞和培养唾液腺癌细胞的β-连环蛋白，用电镜观察β-连环蛋白在胞浆和胞核中异常聚集的和未聚集的两种物质，并对β-Catenin在细胞质和细胞核中是否异常积累进行了检索.口腔癌细胞β-连环蛋白基因突变的检测提取培养的扁平角质层癌细胞和培养的唾液腺癌细胞的β-Catenin的RNA，用PCR-SSCP方法检索基因突变的存在，另外尝试直接测序法Denco突变，并尝试位置的确定。在培养的口腔癌细胞中，β-Catenin在细胞质和细胞核中异常积累的细胞中，不存在β-Catenin基因突变的细胞，也不存在β-Catenin基因突变的细胞。此外，该区域被GSK 3磷酸化。