Development research into molecular target diagnosis and treatment method of cancer of oral cavity that makes Wnt signaling factor target

尽管口腔癌的诊断和治疗方法有了显著的进步，但人们仍然认为口腔癌是一种令人恐惧的致命疾病，面对口腔癌从病态到生活质量的转变存在很大的障碍。虽然β-Catenin被认为是口腔癌的致癌基因之一，但对Wnt细胞信号通路与口腔癌之间关系的研究很少。认为在克服口腔癌早期阶段的紧急情况下，有必要推动这项研究，并进行了以下检查，并取得了成效。培养口腔癌细胞b -连环蛋白细胞质及细胞核不规则积聚的检测。2.无论是β-Catenin在细胞质和细胞核中异常积累的是什么，还是没有积累的东西，用荧光抗体标记来识别培养的扁平角质层癌细胞和培养的唾液腺癌细胞的β-Catenin，用电镜观察，并检索β-Catenin是否在细胞质和细胞核中异常积累。培养口腔癌细胞β-连环蛋白基因突变的检测。提取培养平面角质层癌细胞和培养唾液腺癌细胞的β-Catenin RNA，采用PCR-SSCP法检索基因突变是否存在，另外尝试直接序列法Denco突变，并尝试确定位置。在培养的口腔癌细胞质和细胞核中存在异常积累β-Catenin的细胞，存在β-Catenin基因突变的细胞和不存在β-Catenin基因突变的细胞。此外，该区域是GSK3磷酸化的部分。

It is thought that the cancer of oral cavity is still feared as a fatal disease ease regardless of remarkable advancement of the diagnosis and the treatment method of the cancer of oral cavity and a big obstacle is given from the change to a morbid state of the face it to QOL. Little research of the relation between the cancer of oral cavity and the Wnt cell signaling route is advanced though β-Catenin is thought to be one of the oncogenes. It was thought that it was necessary to promote this research in the emergency for overcoming at the early stage of the cancer of oral cavity, examined as follows, and it reaped the benefit.1. Examination of irregular accumulation to cytoplasm and nucleus of B-Catenin of culture mouth cancer cell.Both what β-Catenin has accumulated abnormally in the cytoplasm and the nucleus and the thing that had not accumulated the label by the fluorescent antibody to recognize β-Catenin of the culture flatness cuticle cancer cell and the culture saliva glandular carcinoma cell, the observation with the electron microscope, and the retrieval whether β-Catenin had accumulated abnormally in the cytoplasm and the nucleus existed.2. Examination of gene mutation of β-Catenin of culture mouth cancer cell. RNA of β-Catenin of the culture flatness cuticle cancer cell and the culture saliva glandular carcinoma cell was extracted, the presence of the gene mutation was retrieved by the PCR-SSCP method, direct sequence method Denco mutation was tried in addition, and the decision of the position was tried. In the culture mouth cancer cell that had abnormally accumulated β-Catenin in the cytoplasm and the nucleus, the one with the gene mutation of β-Catenin and the one that not was existed. Moreover, the area was a part phosphorylated by GSK3.