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Inverted Confocal Laser Scanning Microscope

倒置共焦激光扫描显微镜

基本信息

批准号：
441730715
负责人：
金额：
--
依托单位：
Friedrich-Alexander-Universität Erlangen-Nürnberg
依托单位国家：
德国
项目类别：
Major Research Instrumentation
财政年份：
2020
资助国家：
德国
起止时间：
2019-12-31 至无数据
项目状态：
未结题

来源：
https://gepris.dfg.de/gepris/projekt/441730715?language=en
关键词：
Inverted Confocal Laser Scanning Microscope

项目摘要

In this proposal we apply for the replacement of a confocal laser scanning microscope, which now is in use for more than 10 years. More than 40 research groups made use of the instrument. The present instrument served 41 PhD thesises, more than 100 conference contributions and at least 35 peer reviewed publications. Systematic monitoring of publications only was performed within the last four years, which means the outcome of publications is likely to be much higher.The replacement instrument will be used on different scientific backgrounds such as medical, natural science (anima, planta) and material science. Independend from the plethora of individual extremly versatile backgrounds a few common requirements are linked to the majority of all proposed research. The instrument will be used for multi-channel (3 -4 in average) image acquisition of fixed samples (cells, tissue, crystalline surfaces) in 2 and 3 dimensions. The instrument must deliver high-resolution down to Abbe's limitation, but no super resolution. Emission technology must enable to fine tune the detection range of various emission spectra, even from within the same fluorescent dye changing behaviour under different conditions. A mixture of conventional photomultiplying tubes (PMT) and high sensitive detectors. Excitation in the visible spectrum at all major standard fluorescent excitation labels must be possible. Big physical field of view, in particular for imaging tissues and surfaces with less tiles and hence less stitching to apply. Further a high precision auto focus must be in place for best threedimenional (z-stack) imaging. All these requires a fully automated microscope stage.

在本提案中，我们申请更换共焦激光扫描显微镜，该显微镜现已使用超过10年。40多个研究小组使用了该仪器。目前的工具服务于41博士论文，100多个会议的贡献和至少35同行评审的出版物。仅在过去四年内对出版物进行了系统监测，这意味着出版物的结果可能会高得多。替代仪器将用于不同的科学背景，如医学，自然科学（动物，植物）和材料科学。独立于过多的个人极端多才多艺的背景，一些共同的要求与大多数拟议的研究有关。该仪器将用于固定样本（细胞、组织、晶体表面）的二维和三维多通道（平均3 - 4个）图像采集。该仪器必须提供高分辨率下降到阿贝的限制，但没有超分辨率。发射技术必须能够微调各种发射光谱的检测范围，即使是在不同条件下改变行为的相同荧光染料内。传统光电倍增管（PMT）和高灵敏度探测器的混合物。在所有主要标准荧光激发标记处的可见光谱中的激发必须是可能的。大的物理视野，特别是用于成像组织和表面较少的瓷砖，因此较少的缝合应用。此外，高精度自动对焦必须到位，以获得最佳三维（z堆栈）成像。所有这些都需要一个完全自动化的显微镜载物台。