New autocrine agent in hepats cytes and its role

新型肝细胞自分泌剂及其作用

• 批准号: 04670414
• 负责人: MINE Tetsuya
• 金额: $ 1.41万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670414/
• 关键词: Activin A; TGF beta; Ba chain; Cytoplasmic free calciom concentration; PTX; growth factor; Inhibition of growth; auto crine; ActivinA; TGFβ; β_A鎖; Activin A; Inhibin; TGF β; inositol-1,4,5-trisphosphates; Pertussis toxin; AngiotensinII; バ

I firstly demonstrated that activin A plays a important role in hepatic metabolism, previously. There fore, I started to identify the role of activin A except the effect of hepatic metabolism. Firstly, it was demonstrated that activin A inhibits the proliferation of hepats cytes. Second, activin A seems to be produced by hepatscyles and hepatscytes possesses own specific receptor and sercne thieome hinase. Furthermore, we demonstrated sipal trans duction of activin A in hepatscytes, Activin A activates Phospholipase Cr and promotes the production of inositol tris phosyhates. So, activin A increases the cytsplasmi free calcium concentration. These signal transduction systems were blocked by pertussis toxin.

在此之前，我首先证明了激活素A在肝脏代谢中起重要作用。于是，我开始认识到激活素A在肝脏代谢作用之外的作用。首先，证实激活素A抑制肝细胞的增殖。其次，激活素A似乎是由肝细胞产生的，肝细胞有自己的特异性受体和血清酶。此外，我们证明了激活素A在肝细胞中的sipal转导，激活素A激活磷脂酶Cr并促进肌醇三磷酸的产生。因此，激活素A增加了胞浆游离钙浓度。这些信号转导系统被百日咳毒素阻断。

项目成果

Tomonaga T et al.: "Iso flavonoids, genistein, psi-tectorigenin and orobol increase cyto plasmic free calcium concentration in isolated rat hepato cytes." Biochom.Biophys.Res.Commun. 182. 894-899 (1992)

Tomonaga T 等人：“异类黄酮、金雀异黄酮、psi-鸢尾黄酮和奥罗博尔可增加离体大鼠肝细胞中的细胞质游离钙浓度。”

Yasuda H et al: "Activin A : An autocrine inhibitor of initation of DNA synthesis" J.Clin.Invest.92. 1491-1496 (1993)

Yasuda H 等人：“激活素 A：DNA 合成启动的自分泌抑制剂”J.Clin.Invest.92。

Mine et al.: "Healing of chrome gastric ule depbnds on gastric mucosal prostahlandm syrthesis" Hepato-gastroenterology. (1992)

Mine 等人：“铬胃溃疡的愈合取决于胃粘膜前列腺合成”肝胃肠病学。

Yasuda H et al.: "Evidence of activin a in A-and D-cells, of rat pancreatic is let." Endocrinology. 133. 624-630 (1993)

Yasuda H 等人：“大鼠胰腺 A 细胞和 D 细胞中存在激活素 a 的证据已公布。”

Yasuda H et al.: "Evidence of activin A in A-and D-cells of rat pancreatic islet." Endocrinology. 133. 624-630 (1993)

Yasuda H 等人：“大鼠胰岛 A 细胞和 D 细胞中存在激活素 A 的证据。”