Analysis of Regulation of intercellular junctional system of gastric mucosa

胃粘膜细胞间连接系统的调控分析

• 批准号: 16590635
• 负责人: MINE Tetsuya
• 金额: $ 1.98万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590635/
• 关键词: intercellular junction apparatus; connexin32; knockout mouse; adherence junction; tight junction; gap junction; desmosome; ギャップジャンクション; クロウディン; ZO1; ZO2; カドヘリン; カテニン

There are four types of intercellular junction apparatuses : Desmosome, Tight junction, Adeherence junction and Gap junction. We investigated the mutual dependency of the development of these intercellular junction apparatuses. We checked each apparatus in gastric mucosa by electronmicroscopy using connexin32 knockout mouse. In the connexin32 knockout mouse, we could not detect gap junction in gastric mucosa. In contract, we could detect the desmosomes, tight junctions, and adherence junctions on gastric mucosa of connexin32 knockout mouse. From these findings, we concluded the followings : 1) To make connexin32, a protein of gap junction, knock out, no substitution for connexin32 occurs, even though there are many kinds of gap junction protein. 2) Even if making gap junction delete, no influence on desmosomes, tight junctions, and adherence junctions occurs.

细胞间连接装置有四种类型：桥粒、紧密连接、粘附连接和缝隙连接。我们研究了这些细胞间连接装置的发展的相互依赖性。采用Connexin32基因敲除小鼠，电镜观察胃粘膜各结构。在Connexin32基因敲除小鼠中，我们不能检测到胃粘膜间隙连接。而Cx32基因敲除小鼠胃粘膜上可检测到桥粒、紧密连接和粘附连接。从这些发现中我们得出以下结论：1）尽管缝隙连接蛋白有多种，但敲除缝隙连接蛋白32，并没有发生缝隙连接蛋白32的替代。2)即使使间隙连接缺失，对桥粒、紧密连接和粘附连接也无影响。

项目成果

Virulent Klebsiella preumoniae strains and liver abscess with metastatic lesions

强效克雷伯菌菌株和伴有转移性病变的肝脓肿

• 发表时间: 2005
• 期刊: Hepatol Res 31
• 作者: Zhou;X.;Mine T.
• 通讯作者: Mine T.

Fatal liver failure due to reactivation of lamivudine-resistant HBV mutant

• DOI: 10.3748/wjg.v10.i11.1686
• 发表时间: 2004-06-01
• 期刊: WORLD JOURNAL OF GASTROENTEROLOGY
• 影响因子: 4.3
• 作者: Kagawa, Tatehiro;Watanabe, Norihito;Mine, Tetsuya
• 通讯作者: Mine, Tetsuya

Mitochondrial DNA mutation and functional dyspepsia

线粒体DNA突变与功能性消化不良

• 发表时间: 2005
• 期刊: Intern Med 44・2
• 作者: Isao Okazaki;et al.;Mine T;稲垣 豊;Mine T
• 通讯作者: Mine T

Effect of in vitro interferon-beta administration on hepatitis C virus in peripheral blood mononuclear cells as a predictive....

体外干扰素-β给药对外周血单核细胞中丙型肝炎病毒的影响作为预测......

• 发表时间: 2004
• 期刊: World J Gastroenterol 10.5
• 作者: Mochizuki K;Kagawa T;Takashimizu S;Kawazoe K;Kojima S;Mine T
• 通讯作者: Mine T

シュミレイション内科 上部消化管疾患を探る

模拟内科：探索上消化道疾病

• 发表时间: 2004
• 作者: Sakata R;他6名;Tanaka K.;峯 徹哉
• 通讯作者: 峯 徹哉