Analysis of Regulation of intercellular junctional system of gastric mucosa

细胞间连接装置有四种类型：桥粒连接、紧密连接、Adeherence连接和Gap连接。我们研究了这些细胞间连接装置发展的相互依赖性。我们用连接蛋白32基因敲除的小鼠对胃粘膜中的每个器官进行了电子显微镜检查。在连接蛋白32基因敲除的小鼠中，我们没有检测到胃粘膜的缝隙连接。与之相比，Cx32基因敲除小鼠胃粘膜上可见桥粒、紧密连接和粘连连接。根据这些发现，我们得出以下结论：1)要使缝隙连接蛋白Cx32被敲除，即使有多种缝隙连接蛋白，也不会发生Cx32的替代。2)即使删除缝隙连接，也不影响桥粒、紧密连接和粘连连接的发生。

There are four types of intercellular junction apparatuses : Desmosome, Tight junction, Adeherence junction and Gap junction. We investigated the mutual dependency of the development of these intercellular junction apparatuses. We checked each apparatus in gastric mucosa by electronmicroscopy using connexin32 knockout mouse. In the connexin32 knockout mouse, we could not detect gap junction in gastric mucosa. In contract, we could detect the desmosomes, tight junctions, and adherence junctions on gastric mucosa of connexin32 knockout mouse. From these findings, we concluded the followings : 1) To make connexin32, a protein of gap junction, knock out, no substitution for connexin32 occurs, even though there are many kinds of gap junction protein. 2) Even if making gap junction delete, no influence on desmosomes, tight junctions, and adherence junctions occurs.