Laser Effect on Hard Tissue Formation

激光对硬组织形成的影响

• 批准号: 04671253
• 负责人: YAMADA Keiko
• 金额: $ 1.34万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671253/
• 关键词: He-Ne Laser; Osteoblastic Cells; Hard Tissue Formation; Cell Proliferation; Cell Morphology

The purpose of the present study was to investigate the mineral formation effect of He-Ne laser on clonal osteoblastic cells (MC3T3-E1) when the cells were exposed to He-Ne laser with short duration time (3 min. or 5 min.) as cell growth was not enhanced.The following results were obtained.1. There was no difference in cell morphology observed under microscopy between irradiated cells and unirradiated cells.2. Cell proliferation was not accelerated by He-Ne laser irradiation at the duration time of 3 minutes as well as 5 minutes.3. Amount of calcium accumulated in MC3T3-E1 cell culture was increased by He-Ne laser irradiation compared with control.

本研究旨在观察He-Ne激光短时间（3 min或5 min）照射对克隆成骨细胞（MC 3 T3-E1）矿化的影响。而细胞生长并没有得到促进.光镜下观察照射前后细胞形态无明显差异. He-Ne激光照射3分钟和5分钟后，细胞增殖没有加速。He-Ne激光照射可使MC 3 T3-E1细胞内钙离子的积累量增加。

项目成果

山田 恵子: "He-Neレーザーの骨芽細胞における硬組織促進効果の機序について" 日本レーザー歯学会誌. 4. (1992)

Keiko Yamada：“He-Ne 激光对成骨细胞的硬组织促进作用的机制”，日本激光牙科学会杂志 4。（1992 年）。

KEIKO YAMADA, CHIKA OGINO, KANO SAIJO AND KIKUO KAMIYAMA: "He-Ne Laser Effect on Hard Tissue Formation in Osteoblastic Cells" J.Japanese Society for Laser Dentistry. 5. (in print) (1994)

KEIKO YAMADA、CHIKA OGINO、KANO SAIJO 和 KIKUO KAMIYAMA：“He-Ne 激光对成骨细胞硬组织形成的影响”J.日本激光牙科学会。

KEIKO YAMADA: "He-Ne Laser Effect on Hard Tissue Formation in Osteoblastic Cells" 日本レーザー歯学会誌. 5(未定). (1994)

KEIKO YAMADA：“氦氖激光对成骨细胞硬组织形成的影响”，日本激光牙科学会杂志 5（待定）。