BASIC RESEARCH ON GENE THERAPY FOR HUMAN LEUKEMIAS BY ANTISENSE OLIGODEOXYNUCLEOTIDES

反义寡脱氧核苷酸治疗人类白血病的基础研究

• 批准号: 04671531
• 负责人: MAEKAWA Taira
• 金额: $ 1.41万
• 依托单位: KYOTO PREFECTURAL UNIVERSITY OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671531/
• 关键词: ANTISENSE; LEUKEMIA; MOLECULARLY TARGETTED THERAPY; 遺伝子治療

1. Establishment of large-scale synthesizing and purification methods for antisense oligonucleosides phosphorothioates (1993)2. Evaluation of cell-membrane permeability of antisense oligonucleotides (1993)3. Examination of non-specific toxic effects on hematopoietic progenitors by antisense oligonucleotides (1993)4. Confirmation of clonal suppression on hemopoietic progenitor growth of chronic myeloid leukemia cells by BCR-ABL antisense oligonucleotides(1993, 1994)5. Evaluation of sequence specificity of BCR-ABL antisense oligonucleoside phosphorothioates on growth suppression of chronic myeloid leukemia cells (1994)6. BCR-ABL antisense oligonucleotides caused apoptotic cell death of chronic myeloid leukemia cells BV173, and confirmation of reduced production of p210 chimeric protein by these antisense oligomers (1994)7.Effects of BCR-ABL antisense oligonucleotides on the survival of BV173 leukemic cell transplanted mice are now under investigation (1994)

1. 1 .反义寡核苷磷酸酯大规模合成纯化方法的建立（1993）。反义寡核苷酸细胞膜通透性的评价（1993）反义寡核苷酸对造血祖细胞非特异性毒性作用的研究（1993）BCR-ABL反义寡核苷酸克隆抑制慢性髓系白血病细胞造血祖细胞生长的研究（1993,1994）BCR-ABL反义寡核苷磷酸酯对慢性髓系白血病细胞生长抑制的序列特异性评价（1994）BCR-ABL反义寡核苷酸引起慢性髓系白血病细胞BV173的凋亡细胞死亡，并证实这些反义寡聚物减少了p210嵌合蛋白的产生（1994）7。目前正在研究BCR-ABL反义寡核苷酸对BV173白血病细胞移植小鼠存活的影响（1994）

项目成果

Furukawa,T.: "Establishment of a new cell line with characteristies of a multi-potential progenitor from a patient with chronic myelogenous leukemie in early erythroblastic crisis" Leukemie. 8. 171-180 (1994)

Furukawa,T.：“从患有早期成红细胞危机的慢性粒细胞白血病患者中建立具有多潜能祖细胞特征的新细胞系”Leukemie。

Tamura,M.: "Reduction of the leukemogenic potential of malignant leukemic cells by in vivo treatment with recombinant human granulocyle colony stimulating factor" Leukemia Research. 17. 593-600 (1993)

Tamura,M.：“通过重组人粒细胞集落刺激因子体内治疗降低恶性白血病细胞的致白血病潜力”白血病研究。

前川 平: "癌と遺伝.臨床遺伝医学IV" 古庄敏行,清水信義,北川照男,武部啓, 372 (1993)

Taira Maekawa：“癌症与遗传学。临床遗传医学 IV”Toshiyuki Furusho、Nobuyoshi Shimizu、Teruo Kitakawa、Kei Takebe，372 (1993)

前川 平: "Antisenss oligornuclectideによる白血病細胞の増殖抑制" 血液・腫瘍科. 28. 64-77 (1994)

Hiroshi Maekawa：“反义寡核苷酸对白血病细胞增殖的抑制”，血液学和肿瘤学系，28. 64-77 (1994)。

前川 平: "癌と遺伝、臨床遺伝医学IV" 古庄敏行、清水信義、北川照男、武部 啓, 372 (1993)

Taira Maekawa：“癌症与遗传学，临床遗传医学 IV” Toshiyuki Furusho、Nobuyoshi Shimizu、Teruo Kitakawa、Kei Takebe，372 (1993)