Pathophysiological and morphological changes in the chronic entrapment neuropathy

慢性卡压性神经病的病理生理学和形态学变化

• 批准号: 05671227
• 负责人: SAKADA Takanori
• 金额: $ 0.9万
• 依托单位: SAITAMA MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671227/
• 关键词: peripheral nerve; experimental model; myelin; entrapment neuropathy; blood flow; 絞扼性神経障害; 血流; マイクロスフェアー

Experiment 1 : New model for entrapment neuropathyWe made a new model of entrapment neuropathy which is more physiologically designed. The screw was inserted to the cubital tunnel through the center of medial epicondyle from anterior aspect of supracondylar bone of the humerus in adult rabbit. The ulnar nerve was not exposed at all by this procedure.Results : Early histological findings of the nerve were thickenning of the epineurium and perineurium and proriferation of the subperineural fibrous tissue in 20 months. After 22 months, thinning of the myelin nad shift of small fiber size in the fiber histogram were observed locally in the endoneurium. These findings is similar with the histology of early stage in human chronic entrapment neuropathy.Experiment 2 : Measurement of the sciatic nerve blood flow using a lasser doppler flowmetoey (LDF) in experimental moels for entrapment neuropathy.We tried to measure the intraneural blood flow by the noninvasive colored microshare injection method. Howevr, the trial was failed because of small volume of the neural tissue at the entrapment point. As an experimental model for entrapment neuropathy, we adopted the Mackinnon's method in the sciatic nerve of rabbit.Resuls : Morphological and physiological of this experimental model were similar with the human chronic entrapment neuropathy. Blood flow was reduced from 55% to 85% of that of the normal sciatic nerve at the entrapment point.

实验一：新型压迫性神经病模型我们制作了一种新型的压迫性神经病模型。将螺钉从成年家兔肱骨髁上骨前方经肱骨内上髁中心插入肘管。结果：术后20个月，尺神经早期组织学表现为神经外膜、神经束膜增厚，神经束膜下纤维组织增生。22个月后，在神经内膜中局部观察到髓鞘变薄和纤维直方图中小纤维尺寸的移位。实验二：激光多普勒血流仪（LDF）测定大鼠坐骨神经卡压性损伤模型的坐骨神经血流量：采用彩色微量注射法，对坐骨神经内的血流量进行了无创性测定。然而，试验失败，因为在卡压点的神经组织体积小。采用Mackinnon法建立兔坐骨神经卡压性神经病模型，结果：该模型在形态学和生理学上与人慢性卡压性神经病相似。压迫点的血流量从正常坐骨神经的55%减少到85%。

项目成果

日野原真一、坂田悍教: "慢性絞扼性末梢神経障害の病態" 日手会誌. 11(予定). (1994)

Shinichi Hinohara，Takenori Sakata：“慢性绞窄性周围神经病的病理学”，Nittei Society 杂志 11（预定）。