Pathophysiological and morphological changes in the chronic entrapment neuropathy

实验一：卡压性神经病的新模型我们制作了一种更具生理学设计的新型卡压性神经病模型。将该螺钉从成年兔的肱骨髁上前侧穿过内侧上髁中心置入肘管内。结果：20个月后神经的早期组织学表现为神经外膜和神经外膜增厚，神经周围纤维组织剥离。22个月后，在神经内膜可见髓鞘变薄，纤维直方图上的小纤维移位。这些发现与人类慢性卡压神经病变早期的组织学相似。实验2：在慢性卡压神经病变的实验模型上，用激光多谱勒血流(LDF)测量坐骨神经血流量。然而，试验失败了，因为卡压点的神经组织体积很小。采用Mackinnon‘s法制作兔坐骨神经卡压性神经病的实验模型。结果：该实验模型的形态和生理特征与人类慢性卡压性神经病相似。卡压点的血流量由正常坐骨神经的55%降至85%。

Experiment 1 : New model for entrapment neuropathyWe made a new model of entrapment neuropathy which is more physiologically designed. The screw was inserted to the cubital tunnel through the center of medial epicondyle from anterior aspect of supracondylar bone of the humerus in adult rabbit. The ulnar nerve was not exposed at all by this procedure.Results : Early histological findings of the nerve were thickenning of the epineurium and perineurium and proriferation of the subperineural fibrous tissue in 20 months. After 22 months, thinning of the myelin nad shift of small fiber size in the fiber histogram were observed locally in the endoneurium. These findings is similar with the histology of early stage in human chronic entrapment neuropathy.Experiment 2 : Measurement of the sciatic nerve blood flow using a lasser doppler flowmetoey (LDF) in experimental moels for entrapment neuropathy.We tried to measure the intraneural blood flow by the noninvasive colored microshare injection method. Howevr, the trial was failed because of small volume of the neural tissue at the entrapment point. As an experimental model for entrapment neuropathy, we adopted the Mackinnon's method in the sciatic nerve of rabbit.Resuls : Morphological and physiological of this experimental model were similar with the human chronic entrapment neuropathy. Blood flow was reduced from 55% to 85% of that of the normal sciatic nerve at the entrapment point.