大脳新皮質の非錐体細胞の機能的多様性

新皮质非锥体细胞的功能多样性

• 批准号: 05680712
• 负责人: 川口 泰雄
• 金额: $ 1.34万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05680712/
• 关键词: 大脳皮質; 新皮質; 非錐体細胞; GABA; カルシウム結合蛋白質

大脳新皮質の非錐体細胞は,その形態的・免疫組織化学的多様性からみると,生理的多様性があると思われるが,全く知られていない.本研究では,前頭皮質の脳切片標本で,微分干渉顕微鏡による直視下での細胞同定・ホールセル記録・細胞内染色・免疫組織化学の技術を組み合わせて,新皮質V層の非錐体細胞について,次の三点について明らかにした。(1)非錐体細胞の電流-電圧カーブ・スパイク発射様式等の生理学的性質から、非錐体細胞のなかには、少なくとも二種類の生理的サブタイプがある。一つは、Low Thrshold Spike cell(LTS cell)で、もう一つは、Fast Spiking cell(FS cell)である。(2)非錐体細胞の二種類の生理的サブタイプは軸索分布のパターンが異なり、層構造・垂直構造に関連して神経支配様式が違う。(3)非錐体細胞の免疫組織学的マーカーである二種類のカルシウム結合蛋白質(parvalbumin,calbindin D28K)は、V層では、別の細胞に存在し、非錐体細胞の生理的サブタイプと相関している。二種類のカルシウム結合蛋白質を持つ細胞はV層では、抑制性伝達物質GABAにも陽性なので、二種類の非錐体細胞はGABA作働性の抑制性ニューロンと考えられる。これらから、大脳新皮質V層のGABA作働性の抑制性非錐体細胞は、発火モード・軸索分布・神経活性物質の上から、少なくとも二種類に機能分布していると思われる。

The new hippopotamus is not the body cell, the morphological immuno-histochemistry is multiplicity, the physiology is polygamous, and the whole body is full of knowledge. In this study, the tissue sections of the front skin were used as the standard, and the differential micrographs were observed under the direct view of the microscope. the immunocytochemical technique of intracellular staining was used to detect the immunocytochemistry of the tissue. The new skin V was not isolated from the body, and the second three-point cycle was clear. The main results are as follows: (1) the physiological characteristics of the physiology, such as non-body current, radio current, radiation and other physiology, such as non-body current, non-body current, radiation and so on. One, Low Thrshold Spike cell (LTS cell), one, Fast Spiking cell (FS cell), please. (2) the distribution of two kinds of physiological pathogens in non-somatosomal cells, such as the distribution of trophoblasts, and the vertical creation of the animals under the control of the gods. (3) parvalbumin,calbindin D28K binding protein, V-binding protein, other cell binding protein in non-bovine cytoskeletal immunology, and non-parasomal cytosolic physiology. The combination of two kinds of antigens, the recombinant protein, the inhibitor GABA, and the two kinds of non-recombinant proteins, GABA, were used as inhibitors for sexual inhibition. The distribution of inhibitive non-sterile cells, the distribution of active substances, and the distribution of the two kinds of active substances, such as Ligusticum and oligodeoxynucleotides, can be used as the basis for the distribution of active substances, such as the number of active substances, and the number of active substances.

项目成果

Yasuo Kawaguchi: "Groupings of nonpyramidal andpyramidal cells with specific physiological and morphological characteristics in rat frontal cortex." Journal of Neurophysiology. 69. 416-431 (1993)

Yasuo Kawaguchi：“大鼠额叶皮层中具有特定生理和形态特征的非锥体细胞和锥体细胞群。”

Yasuo Kawaguchi: "Correlation of physiological subgroupings of nonpyramidal cells with parvalbumin-and calbindin D28k-immunoreactive neurons in layer V of rat frontal cortex." Journal of Neurophysiology. 70. 387-396 (1993)

Yasuo Kawaguchi：“大鼠额叶皮层 V 层中非锥体细胞的生理亚群与小清蛋白和钙结合蛋白 D28k 免疫反应性神经元的相关性。”