Laboratory Suncus as Useful Animal Model of Focal Tonillar Infection

由于目前常规的实验室小型动物，如小鼠、大鼠和豚鼠扁桃体闭锁，基本的扁桃体模型实验很少。在此条件下，本研究选择了新近培育的食虫目实验动物--臭鼩（Suncus murinus）。这种动物有一对原始的腭扁桃体和管状扁桃体。免疫电镜观察表明，扁桃体由淋巴上皮细胞和淋巴结组成。高内皮微静脉和传出神经分别位于滤泡旁带和结节周围区。用我们新近研制的抗Suncus T和B淋巴细胞单克隆抗体对扁桃体冰冻切片进行染色，发现T细胞位于滤泡旁带，B细胞位于结节内。在目前的鼓室内HRP致敏，许多HRP抗体产生细胞不仅在输卵管扁桃体，但也在其区域淋巴结，脾和腭扁桃体。在肾小球内很少检测到相同的HRP抗体。这些结果表明，臭鼩 鼱是一种值得作为人类继发性扁桃体局灶性感染的模型动物。

Since the present conventional small laboratory amimals ; mice, rats and guinea pigs lock tonsil, basic tonnsillar model experiments are very little. Under such conditions, Suncus (suncus murinus), recently developed as a new laboratory animal of Insectivora was selected is this research. The animals has a pair of primitive plalatine- and tubal-tonsils. The present immuno-electron microscopical study revealed that the individual tonsil consisted of lymphoepithelial symbiosis containing M cells and lymphnodule. The high-endothelial venules and efferent lymphatics were situated in the parafolliclar zone and peri-nodullar region, respectively. Staining of the frozen tonsil sections by our recently developed anti-Suncus T and B lymphocyte monoclonal antibodies revealed that T cells occupied in the parafollicular zone and B cells localized within the nodules. In the present intra-tympanic cavity HRP sensitization, numerous HRP-antibody producing cells were seen not only in the tubal tonsils but also in their regional lymph nodes, spleen and palatine tonsils. The same HRP antibody was rarely detected within the kidney glomeruli. These findings presume that Suncus is worthy as a model animal of human secondary focal-tonsillar infection.