权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Laboratory Suncus as Useful Animal Model of Focal Tonillar Infection

实验室 Suncus 作为局灶性扁桃体感染的有用动物模型

基本信息

批准号：
05680744
负责人：
KIMURA Michio
金额：
$ 0.9万
依托单位：
Kansai College of Oriental Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05680744/
关键词：
Laboratory Suncus Palatine Tonsil Tubal Tonsil Monoclonal Antibody Suncus T cell Suncus B cell Animal model 免疫組織学リンパ上皮食虫目扁桃疾患モデル接着因子スンクス・リンパ球抗体微細構造

项目摘要

Since the present conventional small laboratory amimals ; mice, rats and guinea pigs lock tonsil, basic tonnsillar model experiments are very little. Under such conditions, Suncus (suncus murinus), recently developed as a new laboratory animal of Insectivora was selected is this research. The animals has a pair of primitive plalatine- and tubal-tonsils. The present immuno-electron microscopical study revealed that the individual tonsil consisted of lymphoepithelial symbiosis containing M cells and lymphnodule. The high-endothelial venules and efferent lymphatics were situated in the parafolliclar zone and peri-nodullar region, respectively. Staining of the frozen tonsil sections by our recently developed anti-Suncus T and B lymphocyte monoclonal antibodies revealed that T cells occupied in the parafollicular zone and B cells localized within the nodules. In the present intra-tympanic cavity HRP sensitization, numerous HRP-antibody producing cells were seen not only in the tubal tonsils but also in their regional lymph nodes, spleen and palatine tonsils. The same HRP antibody was rarely detected within the kidney glomeruli. These findings presume that Suncus is worthy as a model animal of human secondary focal-tonsillar infection.

自目前常规小型实验动物；小鼠、大鼠和豚鼠锁住扁桃体，基础扁桃体模型实验很少。在这种条件下，本研究选择了最近发展起来的一种新的食虫科实验动物——太阳cus murinus。这种动物有一对原始的腭状扁桃体和管状扁桃体。目前的免疫电镜研究显示，单个扁桃体由含有M细胞和淋巴结的淋巴上皮共生组成。高内皮小静脉和传出淋巴管分别位于滤泡旁区和结节周围区。我们最近开发的抗suncus T和B淋巴细胞单克隆抗体对冷冻扁桃体切片进行染色，发现T细胞位于滤泡旁区，B细胞位于结节内。在当前的鼓腔内HRP致敏中，不仅在管状扁桃体中发现大量HRP抗体产生细胞，而且在其区域淋巴结、脾脏和腭扁桃体中也发现大量HRP抗体产生细胞。在肾小球中很少检测到相同的HRP抗体。这些结果表明，斑鼠是值得作为人类继发性局灶扁桃体感染的模型动物。