Laboratory Suncus as Useful Animal Model of Focal Tonillar Infection

实验室 Suncus 作为局灶性扁桃体感染的有用动物模型

基本信息

批准号：
05680744
负责人：
KIMURA Michio
金额：
$ 0.9万
依托单位：
Kansai College of Oriental Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05680744/
关键词：
Laboratory Suncus Palatine Tonsil Tubal Tonsil Monoclonal Antibody Suncus T cell Suncus B cell Animal model 免疫組織学リンパ上皮食虫目扁桃疾患モデル接着因子スンクス・リンパ球抗体微細構造

项目摘要

Since the present conventional small laboratory amimals ; mice, rats and guinea pigs lock tonsil, basic tonnsillar model experiments are very little. Under such conditions, Suncus (suncus murinus), recently developed as a new laboratory animal of Insectivora was selected is this research. The animals has a pair of primitive plalatine- and tubal-tonsils. The present immuno-electron microscopical study revealed that the individual tonsil consisted of lymphoepithelial symbiosis containing M cells and lymphnodule. The high-endothelial venules and efferent lymphatics were situated in the parafolliclar zone and peri-nodullar region, respectively. Staining of the frozen tonsil sections by our recently developed anti-Suncus T and B lymphocyte monoclonal antibodies revealed that T cells occupied in the parafollicular zone and B cells localized within the nodules. In the present intra-tympanic cavity HRP sensitization, numerous HRP-antibody producing cells were seen not only in the tubal tonsils but also in their regional lymph nodes, spleen and palatine tonsils. The same HRP antibody was rarely detected within the kidney glomeruli. These findings presume that Suncus is worthy as a model animal of human secondary focal-tonsillar infection.

自目前的常规小型实验室娱乐以来；小鼠，大鼠和几内亚猪锁定扁桃体，基本的Tonnsillar模型实验很少。在这种情况下，这项研究选择了最近作为新型实验室动物开发的Suncus（Suncus Murinus）。这些动物有一对原始的铂和输卵管扁桃体。目前的免疫电子显微镜研究表明，单个扁桃体由含有M细胞和淋巴结的淋巴皮共生。高内皮静脉和传出淋巴管分别位于寄生虫区和诺杜拉尔周围区域。我们最近开发的抗乳糖T和B淋巴细胞单克隆抗体对冷冻扁桃体切片的染色表明，在结节内固定在层状区和B细胞中的T细胞。在目前的脉络膜内腔HRP敏化中，不仅在输卵管扁桃体中，而且在其区域淋巴结，脾和palatine扁桃体中都可以看到许多HRP - 抗体产生细胞。在肾脏肾小球中很少检测到相同的HRP抗体。这些发现假设Suncus值得作为人类继发局灶性扁桃体感染的模型动物。