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Experimental Pathological Evaluation on Laboratory Suncus for Tonsillar Research

用于扁桃体研究的实验室圣库实验病理学评估

基本信息

批准号：
07557026
负责人：
KIMURA Michio
金额：
$ 1.6万
依托单位：
Kansai College of Oriental Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07557026/
关键词：
Tonsillar Immunity Suncus T Cell Suncus B Cell Monoclonal Antibody Palatine Tonsil Tubal Tonsil Animal Model Tonsil Research リンパ球食虫目リンパ上皮食中目

项目摘要

Clinico-pathologically, human tonsils have been marked as an important iducer site of mucosal immunity. Neverthless, the tonnsillar laboratory investigations are very little because the present conventional laboratory animals ; mice, rats and guinea pigs lack tonsils.Under such conditions, a new laboratory animal, Suncus (Suncus Murinus) was selected in this study. The animal has a pair of palatine and tubal tonsils. The individual tonsils consisted of crypt-like lymphoepithelialsymbiosis and single lymph nodules. Their crypt-epithelia contained M cells in the luminal sites. The postcapillary venules and efferent lymphatics localized in the marginal regons. Immunohistochemical preparations stained by our developed anti-Suncus T and B cell monoclonal antibodies revealed that T cells occupied the parafollicular zone and B ells located within the follicles. In the intra-tympanic cavity horseradish peroxidase (HRP) sensitization, numerous HRP-antibody positive B cells were detected not only in the tubal tonsil but rarely in their regional lymph node, spleen and palatine tonsil. Although it is unclear whether the HRP-antibody consists of IgG class or IgA,the presence of M cells and the mesangial HRP-antibody deposition pressume that the antibody partially contain IgA ones. The present study supports that the suncus is worthy for understanding tonsillar regional immunity. We are trying to develope anti-Suncus IgA antibody monoclonal antibody. The IgA monoclonal antibody will bring us further significant information in the tonsil pathology.

临床病理上，扁桃体已被标记为粘膜免疫的重要诱导部位。然而，由于目前常规的实验动物--小鼠、大鼠和豚鼠缺乏扁桃体，吨级的实验室调查很少，在这种情况下，本研究选择了一种新的实验动物--森科斯(Suncus Murinus)。这种动物有一对腭扁桃体和输卵管扁桃体。单个扁桃体由隐窝状淋巴上皮共生和单个淋巴结节组成。它们的隐窝上皮细胞在管腔部位含有M细胞。毛细血管后小静脉和传出淋巴管位于边缘区域。我们研制的抗Suncus T和B细胞单抗免疫组织化学染色显示，T细胞占据卵泡旁带，B细胞位于卵泡内。在鼓室内辣根过氧化物酶(HRP)致敏过程中，不仅在输卵管扁桃体中检测到大量HRP抗体阳性的B细胞，而且在其区域淋巴结、脾和腭扁桃体中也很少检测到HRP抗体阳性的B细胞。虽然目前尚不清楚HRP抗体是由Ig G类还是Ig A类组成，但M细胞的存在和系膜HRP抗体的沉积提示该抗体部分含有Ig A类抗体。本研究支持Suncus对于了解扁桃体区域免疫的价值。我们正在努力研制抗Suncus IgA抗体的单抗。IgA单抗将为我们提供扁桃体病理的更多有意义的信息。