Experimental Pathological Evaluation on Laboratory Suncus for Tonsillar Research

临床病理学上，人类扁桃体已被标记为粘膜免疫的重要诱导部位。然而，由于目前常规的实验动物；小鼠、大鼠和豚鼠没有扁桃体。在这种情况下，本研究选择了一种新的实验动物，Suncus （Suncus Murinus）。这种动物有一对腭扁桃体和管状扁桃体。单个扁桃体由隐窝样淋巴上皮共生和单个淋巴结节组成。它们的隐窝上皮在管腔部位含有M细胞。毛细血管后小静脉和传出淋巴管位于边缘区。我们开发的抗suncus T和B细胞单克隆抗体的免疫组织化学染色显示T细胞占据滤泡旁区，B细胞位于滤泡内。在鼓室内辣根过氧化物酶（HRP）致敏时，不仅在输卵管扁桃体中检测到大量HRP抗体阳性的B细胞，在其局部淋巴结、脾脏和腭扁桃体中也很少检测到。虽然目前尚不清楚hrp抗体是由IgG类还是IgA类组成，但M细胞的存在和系膜的hrp抗体沉积推测抗体部分含有IgA类。本研究支持了斑对了解扁桃体区域免疫的价值。我们正在尝试开发抗太阳的IgA抗体单克隆抗体。IgA单克隆抗体将为我们在扁桃体病理方面提供进一步的重要信息。

Clinico-pathologically, human tonsils have been marked as an important iducer site of mucosal immunity. Neverthless, the tonnsillar laboratory investigations are very little because the present conventional laboratory animals ; mice, rats and guinea pigs lack tonsils.Under such conditions, a new laboratory animal, Suncus (Suncus Murinus) was selected in this study. The animal has a pair of palatine and tubal tonsils. The individual tonsils consisted of crypt-like lymphoepithelialsymbiosis and single lymph nodules. Their crypt-epithelia contained M cells in the luminal sites. The postcapillary venules and efferent lymphatics localized in the marginal regons. Immunohistochemical preparations stained by our developed anti-Suncus T and B cell monoclonal antibodies revealed that T cells occupied the parafollicular zone and B ells located within the follicles. In the intra-tympanic cavity horseradish peroxidase (HRP) sensitization, numerous HRP-antibody positive B cells were detected not only in the tubal tonsil but rarely in their regional lymph node, spleen and palatine tonsil. Although it is unclear whether the HRP-antibody consists of IgG class or IgA,the presence of M cells and the mesangial HRP-antibody deposition pressume that the antibody partially contain IgA ones. The present study supports that the suncus is worthy for understanding tonsillar regional immunity. We are trying to develope anti-Suncus IgA antibody monoclonal antibody. The IgA monoclonal antibody will bring us further significant information in the tonsil pathology.