Usefulness of Suncus as an Animal Model of Otitis Media

Suncus 作为中耳炎动物模型的有用性

基本信息

批准号：
09680837
负责人：
KIMURA Michio
金额：
$ 1.41万
依托单位：
Kansai College of Oriental Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680837/
关键词：
Suncus Tubal Tonsil Otitis Media Animal Model Monoclonal Antibody Palatine Tonsil Mucosal Immunity Suncus IgA 気道免疫 M細胞食虫目中耳炎

项目摘要

A number of animal models were utilized for study of pathogenesis of Otitis Media ( OM ) using mostly conventional laboratory animals ; rat, mouce, guinea pig and chinchilla. Among these laboratory experiments, viral OM models were successfully used for influenza A virus using chin-chilla. The middle ear immunity of these animals is different from those of our human, because they lack tonsils. This did not simulate the human condition underlying reason for the chronic OM. Under such conditions, we here introduced Suncus ( suncus murinus ) a new laboratory animal which has tubal-tonsils in the middle ear and paratine ones in their fauces. Phylogenically the animal is closer to our primates. The size of suncus is almost same as those of young rats. Recently we have newly developed the anti-suncus T lymphocytes-, IgM- and IgG-monoclonal antibodies. Current data suggests that major middle ear immunoglobulins found in chronic OM include IgG, IgM and IgA, and immune protection is conerred largely by IgG and IgM. While the role of IgA in OM is not yet fully established, although thereis evidence that bacterial adherence may be prevented by secretory IgA. Delayed development in the serum bacterial antibody response is suggested to be the underlaying reason for OM susceptivility.During the present study, we found that an anti-mouce IgA polyclonal antibody had a strong cross.Using these IgM, IgG, IgA and T lympnocyte antibodies, the suncus middle ear immune response against artificial antigens (HRP, P6) were examined. And the obtained significant data were reorted the international symposia on animal model of OM.

许多动物模型用于研究大多数常规实验动物的中耳炎（OM）发病机理。老鼠，穆斯，豚鼠和龙猫。在这些实验室实验中，病毒OM模型成功地用于使用Chin-Chilla的流感病毒。这些动物的中耳免疫与我们人类的免疫力不同，因为它们缺乏扁桃体。这没有模拟慢性OM的人类状况。在这种情况下，我们在这里引入了Suncus（Murinus Suncus）一种新的实验室动物，该动物在中耳耳朵中有管ub，在旁边的旁边占据了旁耳的。从系统发作上，动物更接近我们的灵长类动物。日气的大小几乎与年轻大鼠的大小相同。最近，我们新开发了抗核心T淋巴细胞，IgM和IgG单克抗体。当前数据表明，在慢性OM中发现的主要中耳免疫球蛋白包括IgG，IgM和IgA，并且免疫保护主要由IgG和IgM串联。尽管IgA在OM中的作用尚未完全确定，但有证据表明细菌依从性可以由分泌的IgA预防。 Delayed development in the serum bacterial antibody response is suggested to be the underlaying reason for OM susceptivility.During the present study, we found that an anti-mouce IgA polyclonal antibody had a strong cross.Using these IgM, IgG, IgA and T lympnocyte antibodies, the suncus middle ear immune response against artificial antigens (HRP, P6) were examined.并将获得的重要数据重新进行了OM动物模型的国际研讨会。