Usefulness of Suncus as an Animal Model of Otitis Media

许多动物模型被用来研究中耳炎(OM)的发病机制，主要使用常规的实验动物：大鼠、小鼠、豚鼠和栗鼠。在这些实验室实验中，病毒OM模型成功地用于甲型流感病毒的下丘脑。这些动物的中耳免疫力与我们人类的不同，因为它们缺乏扁桃体。这并没有模拟导致慢性OM的人类条件。在这种情况下，我们在这里介绍了一种新的实验动物Suncus(Suncus Murinus)，它的中耳有输卵管扁桃体，喉部有副扁桃体。在系统发育上，这种动物与我们的灵长类动物更接近。Suncus的大小与幼鼠几乎相同。最近我们又研制出了抗Suncus T淋巴细胞、IgM和Ig G的单抗。目前的资料表明，慢性OM患者中耳免疫球蛋白主要包括免疫球蛋白Ig G、Ig M和Ig A，免疫保护主要由Ig G和Ig M介导。而IgA在OM中的作用尚未完全确定，尽管有证据表明分泌型IgA可能阻止细菌黏附。血清细菌抗体反应迟缓被认为是OM易感性的根本原因。在本研究中，我们发现一种抗鼠IgA多克隆抗体具有很强的交叉。利用这些抗体，我们检测了Suncus中耳对人工抗原(HRP，P6)的免疫反应。并将所获得的有意义的数据用于OM动物模型的国际研讨会。

A number of animal models were utilized for study of pathogenesis of Otitis Media ( OM ) using mostly conventional laboratory animals ; rat, mouce, guinea pig and chinchilla. Among these laboratory experiments, viral OM models were successfully used for influenza A virus using chin-chilla. The middle ear immunity of these animals is different from those of our human, because they lack tonsils. This did not simulate the human condition underlying reason for the chronic OM. Under such conditions, we here introduced Suncus ( suncus murinus ) a new laboratory animal which has tubal-tonsils in the middle ear and paratine ones in their fauces. Phylogenically the animal is closer to our primates. The size of suncus is almost same as those of young rats. Recently we have newly developed the anti-suncus T lymphocytes-, IgM- and IgG-monoclonal antibodies. Current data suggests that major middle ear immunoglobulins found in chronic OM include IgG, IgM and IgA, and immune protection is conerred largely by IgG and IgM. While the role of IgA in OM is not yet fully established, although thereis evidence that bacterial adherence may be prevented by secretory IgA. Delayed development in the serum bacterial antibody response is suggested to be the underlaying reason for OM susceptivility.During the present study, we found that an anti-mouce IgA polyclonal antibody had a strong cross.Using these IgM, IgG, IgA and T lympnocyte antibodies, the suncus middle ear immune response against artificial antigens (HRP, P6) were examined. And the obtained significant data were reorted the international symposia on animal model of OM.