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Usefulness of Suncus as an Animal Model of Otitis Media

Suncus 作为中耳炎动物模型的有用性

基本信息

批准号：
09680837
负责人：
KIMURA Michio
金额：
$ 1.41万
依托单位：
Kansai College of Oriental Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680837/
关键词：
Suncus Tubal Tonsil Otitis Media Animal Model Monoclonal Antibody Palatine Tonsil Mucosal Immunity Suncus IgA 気道免疫 M細胞食虫目中耳炎

项目摘要

A number of animal models were utilized for study of pathogenesis of Otitis Media ( OM ) using mostly conventional laboratory animals ; rat, mouce, guinea pig and chinchilla. Among these laboratory experiments, viral OM models were successfully used for influenza A virus using chin-chilla. The middle ear immunity of these animals is different from those of our human, because they lack tonsils. This did not simulate the human condition underlying reason for the chronic OM. Under such conditions, we here introduced Suncus ( suncus murinus ) a new laboratory animal which has tubal-tonsils in the middle ear and paratine ones in their fauces. Phylogenically the animal is closer to our primates. The size of suncus is almost same as those of young rats. Recently we have newly developed the anti-suncus T lymphocytes-, IgM- and IgG-monoclonal antibodies. Current data suggests that major middle ear immunoglobulins found in chronic OM include IgG, IgM and IgA, and immune protection is conerred largely by IgG and IgM. While the role of IgA in OM is not yet fully established, although thereis evidence that bacterial adherence may be prevented by secretory IgA. Delayed development in the serum bacterial antibody response is suggested to be the underlaying reason for OM susceptivility.During the present study, we found that an anti-mouce IgA polyclonal antibody had a strong cross.Using these IgM, IgG, IgA and T lympnocyte antibodies, the suncus middle ear immune response against artificial antigens (HRP, P6) were examined. And the obtained significant data were reorted the international symposia on animal model of OM.

许多动物模型用于研究中耳炎（OM）的发病机制，主要使用传统的实验室动物;大鼠，小鼠，豚鼠和灰鼠。在这些实验室实验中，使用灰鼠成功地将病毒OM模型用于甲型流感病毒。这些动物的中耳免疫力与我们人类的中耳免疫力不同，因为它们没有扁桃体。这并未模拟慢性OM的潜在原因的人体状况。在这种条件下，我们引进了一种新的实验动物--臭鼩（Suncus murinus），它的中耳有管状扁桃体，耳旁有管状扁桃体。从系统发生学上讲，这种动物更接近我们的灵长类动物。其大小与幼鼠基本相同。近年来，我们又研制了抗淋巴细胞、IgM和IgG的单克隆抗体。目前的数据表明，在慢性OM中发现的主要中耳免疫球蛋白包括IgG、IgM和伊加，并且免疫保护在很大程度上由IgG和IgM控制。虽然伊加在OM中的作用尚未完全确定，但有证据表明分泌型伊加可防止细菌粘附。本研究用抗小鼠伊加多克隆抗体和T淋巴细胞抗体，检测了人工抗原（HRP，P6）对兔中耳的免疫应答。所获得的有意义的数据已在国际OM动物模型研讨会上发表。