Usefulness of Suncus as an Animal Model of Otitis Media

为了研究中耳炎的发病机制，我们使用了许多动物模型，其中大多数是传统的实验动物；老鼠，老鼠，豚鼠和栗鼠。在这些实验室实验中，病毒OM模型成功地应用于甲型流感病毒。这些动物的中耳免疫力与人类不同，因为它们没有扁桃体。这并没有模拟人类慢性OM的潜在原因。在这种情况下，我们在这里引进了一种新的实验动物，它在中耳有管状扁桃体，在它们的龙头有腭状扁桃体。在系统发育上，这种动物更接近我们的灵长类动物。其大小与幼鼠几乎相同。近年来，我们新开发了抗肿瘤的T淋巴细胞单克隆抗体、IgM单克隆抗体和igg单克隆抗体。目前资料显示，慢性OM中耳主要免疫球蛋白包括IgG、IgM和IgA，免疫保护主要由IgG和IgM转化。虽然IgA在OM中的作用尚未完全确定，但有证据表明，分泌IgA可能会阻止细菌粘附。血清细菌抗体反应的延迟发展被认为是OM敏感性的潜在原因。在本研究中，我们发现一种抗小鼠IgA多克隆抗体具有很强的交叉性。采用IgM、IgG、IgA和T淋巴细胞抗体检测兔中耳对人工抗原（HRP、P6）的免疫应答。并在OM动物模型国际研讨会上报道了所获得的重要数据。

A number of animal models were utilized for study of pathogenesis of Otitis Media ( OM ) using mostly conventional laboratory animals ; rat, mouce, guinea pig and chinchilla. Among these laboratory experiments, viral OM models were successfully used for influenza A virus using chin-chilla. The middle ear immunity of these animals is different from those of our human, because they lack tonsils. This did not simulate the human condition underlying reason for the chronic OM. Under such conditions, we here introduced Suncus ( suncus murinus ) a new laboratory animal which has tubal-tonsils in the middle ear and paratine ones in their fauces. Phylogenically the animal is closer to our primates. The size of suncus is almost same as those of young rats. Recently we have newly developed the anti-suncus T lymphocytes-, IgM- and IgG-monoclonal antibodies. Current data suggests that major middle ear immunoglobulins found in chronic OM include IgG, IgM and IgA, and immune protection is conerred largely by IgG and IgM. While the role of IgA in OM is not yet fully established, although thereis evidence that bacterial adherence may be prevented by secretory IgA. Delayed development in the serum bacterial antibody response is suggested to be the underlaying reason for OM susceptivility.During the present study, we found that an anti-mouce IgA polyclonal antibody had a strong cross.Using these IgM, IgG, IgA and T lympnocyte antibodies, the suncus middle ear immune response against artificial antigens (HRP, P6) were examined. And the obtained significant data were reorted the international symposia on animal model of OM.