Immunohistological evaluation of pathogenetic models of IgA nephritis in ddY mice

ddY小鼠IgA肾炎发病模型的免疫组织学评价

• 批准号: 62580035
• 负责人: KIMURA Michio
• 金额: $ 0.9万
• 依托单位: Kansai College of Acupuncture Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62580035/
• 关键词: ddY mouse; IgA nephritis; immunoelectron microscopy; Peyer's patch; 糸球体; lgA腎炎モデル; スンクス; パイエル板; 口蓋扁桃; ddyマウス; HRP抗体; 腎糸球体

Aged femal ddy mice, rerely revealed intense glomerular igA depositions, have att-racted the attention of many investigators as a useful model of human igA nephritis. The present experiments have examined an immunohistological pathogenesis of the igA nephritis induced by Peyer's patch HRP-injection and following intravenous indian ink-injection in the mice. The ink-injection was employed for the artificial block-ing of liver reticulo-endothelial phagocytotic (REP) function. The present HRP and ink-injections resulted mostly a typical depositoin of HRP-antibody in the glomeru-lar submesangial regions. High magnification of the depostion indicated often finger or striple-like structures, described previously in a human igA nephritis. The immunohistological examination of ddy mice revealed that the HRP-antibody deposit-area coincides with the igA accumulated areas in their contrast serial sections. These data probably indicate that an essential participation of REP function in the constant induction of igA nephritis, in relation with the gut-associated igA immunity.

老年雌性ddy小鼠肾小球伊加沉积强烈，作为人类伊加肾炎的一种有用模型已引起许多研究者的注意。本实验研究了免疫组织化学的发病机制的伊加肾炎诱导的派伊尔氏斑HRP注射和静脉注射印度墨水在小鼠。采用墨汁灌注法阻断肝网状内皮细胞吞噬功能。本文所用的HRP和墨汁注射法主要在肾小球系膜下区产生典型的HRP抗体沉积。高倍镜下沉积物常呈指状或条状结构，与人类伊加肾炎相似。ddy小鼠免疫组织化学检查显示，HRP抗体沉积区与免疫球伊加）聚集区在其对比切片上一致。这些数据可能表明，REP的功能在不断诱导伊加肾炎的重要参与，与肠道相关的伊加免疫。

项目成果

Kimura Michio: American Journal of Chinese Medicine. 16. 159-167 (1988)

木村道雄：美国中医杂志。

Kimura Michio: Experimental Animals.

木村道雄：实验动物。

M. Kimura and K. Tohya: Journal of Electron Microscopy. (1988)

M. Kimura 和 K. Tohya：电子显微镜杂志。

Kimura Michio: Anatomischer anzeiger. 165. 143-150 (1988)

木村道雄：解剖学杂志。

M. Kimura; K. Tohya: "Scanning, transmission and immunoelectron microscopical studies of the tonsil-like lymphoid organ of normal and horseradish peroxidase-injected laboratory suncuses." Acta Anatomica. (1989)

M.木村；