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PATHOGENESIS OF PANCREATIC SOTONE : STUDIES ON THE ORGANIC MATRIX OF HUMAN PANCREATIC STONES.

胰腺结石的发病机制：人类胰腺结石有机基质的研究。

基本信息

批准号：
05807111
负责人：
TAKEDA Kazunori
金额：
$ 1.22万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05807111/
关键词：
Pancreatic Stone Chronic Pancreatitis Organic Matrix Extracellular Matrix フィブロネクチン

项目摘要

We treated pancreatic stones just after removal each from pancreatic lithiasis patients to clarify the components of the organic matrix by immunohistochemistry. Stones were fixed in 4% buffered formaldehyde for 24 hours, then decalcified stationary in 0.5N HCI with 0.1M EDTA at room temperature for up to 60 minutes. All stones were decalcified with bubble and firm sponge-like matrices were obtained. Standard staining included H-E,PAS,and alcian blue was made. Immunohistochemical staining with specific fibronectin, laminin, collagen IV,lactoferrin (DAKO), and PSP-reg antibodies were carried out by avidin-biotin complex methods. Control staining with non-immune serum was negative. Standard staining revealed that organic component formed matrix throughout the stone and matrix was heterogeneous in texture such as reticular, lamellar, and hyalin-like including altered cellular constituent. In all seven matrices, there were wide areas intensely stained for fibronectin and laminin. All specimens had positive areas for collagen IV,lactoferrin and PSP-reg, but the degree of intensity and the pattern of distribution were different in each matrix. Process of decalcification in this study is suitable for immunohistochemistry. Fragmentation of matrix occured when decalcification was carried out in EDTA with constant stirring. Organic matrix was found throughout the pancreatic stone. Lactoferrin and PSP-reg were positive in the matrix as were expected. Fibronectin, laminin and collagen IV were positive in the matrix, so extracellular matrix and basement membrane components play an important role in the pathogenesis of the pancreatic stones.

我们用免疫组织化学方法对胰腺结石患者取石后的胰腺结石进行处理，以明确有机基质的成分。石块在4%缓冲甲醛中固定24小时，然后在0.5N盐酸中用0.1M EDTA在室温下脱钙60分钟。所有的结石都用气泡脱钙，得到坚固的海绵状基质。标准染色包括H-E、PAS，并制成阿尔辛蓝。免疫组织化学染色采用抗生物素-亲和素-生物素复合体方法。非免疫血清对照染色为阴性。标准染色显示结石中的有机成分在整个结石中形成基质，基质的质地不均匀，如网状、板层状和透明质样，包括改变的细胞成分。在所有七种基质中，都有纤维连接蛋白和层粘连蛋白的广泛区域被强烈染色。所有标本均有IV型胶原、乳铁蛋白和PSP-reg的阳性区域，但强度和分布模式在每种基质中不同。本研究中的脱钙过程适用于免疫组织化学。在EDTA中持续搅拌进行脱钙时，会出现基质碎裂现象。在整个胰腺结石中发现了有机基质。不出所料，乳铁蛋白和PSP-reg在基质中呈阳性。纤维连接蛋白、层粘连蛋白和IV型胶原在胰腺结石的基质中呈阳性表达，细胞外基质和基底膜成分在胰腺结石的发病机制中起重要作用。