LC/MS system (Mass spectrometer online coupled to HPLC)

LC/MS 系统（质谱仪在线耦合至 HPLC）

• 批准号: 442641014
• 金额: --
• 依托单位: Eberhard Karls Universität Tübingen
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/442641014?language=en
• 关键词: LC; MS; system; Mass; spectrometer

The analytics lab of the Center for Plant Molecular Biology (ZMBP) of the University of Tübingen provides a service platform for metabolomics. These targeted and untargeted metabolite analyses are primarily offered to the research groups of the ZMBP and the CRC 1101, but in addition, as part of the Center for Quantitative Biology (QBiC), they are also provided to all members of the University of Tübingen and its external partners. The analytics lab is run by three permanent scientists and a permanent technician. Any person of the lab is dedicated to a special instrument, thus providing different analyses, but they are able to replace each other. The analyses of very diverse samples from different research areas within the University of Tübingen (e.g. plant biology, microbiology, ecology, biochemistry, geo-­sciences or medical research), are conducted with different analytical instrumentations, among them two liquid chromatography – mass spectrometry (LC/MS) systems. Whereas one LC/MS instrument is mainly used for total unknown metabolomics analysis and structural elucidations, the second one is dedicated to most accurate and reproducible quantitative analyses of selected analytes. The later instrument is meanwhile 15 years old and repair costs and – times have been increasing during the last couple of years. This meanwhile results in a significantly decreased sample throughput and thus longer waiting times for quantitative analyses. In addition, the current system does not offer the analytical sensitivity needed to answer modern research questions in the areas of e.g. (plant) hormones, cytokinins, low abundant amino acids and vitamins, sugar phosphates and phosphorylated peptides. Due to these reasons we herein apply for the replacement of the current system by a modern and more sensitive instrument.

蒂宾根大学植物分子生物学中心（ZMBP）的分析实验室为代谢组学提供了服务平台。这些靶向和非靶向代谢物分析主要提供给ZMBP和CRC 1101的研究小组，但此外，作为定量生物学中心（QBiC）的一部分，它们也提供给蒂宾根大学及其外部合作伙伴的所有成员。分析实验室由三名常任科学家和一名常任技术员管理。实验室的任何人都致力于一个特殊的仪器，从而提供不同的分析，但他们能够相互替代。图宾根大学不同研究领域（如植物生物学、微生物学、生态学、生物化学、地质科学或医学研究）的各种样品的分析使用不同的分析仪器进行，其中包括两种液相色谱-质谱（LC/MS）系统。其中一台LC/MS仪器主要用于总未知代谢组学分析和结构解析，另一台则致力于对选定分析物进行最准确和可重复的定量分析。后一种仪器已经使用了15年，在过去的几年里，维修费用和维修时间一直在增加。这同时导致样品通量显著降低，从而导致定量分析的等待时间更长。此外，目前的系统不提供回答例如（植物）激素、细胞分裂素、低丰度氨基酸和维生素、糖磷酸盐和磷酸化肽领域中的现代研究问题所需的分析灵敏度。由于这些原因，我们在此申请用一种现代化的、更灵敏的仪器取代现有的系统。