High Resolution LC-MS System with Automated Samplepreparation for Biomarker Validation

具有用于生物标志物验证的自动样品制备功能的高分辨率 LC-MS 系统

• 批准号: 453505224
• 金额: --
• 依托单位: Ludwig-Maximilians-Universität München (LMU)
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2021
• 资助国家: 德国
• 起止时间: 2020-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/453505224?language=en
• 关键词: Resolution; LC; MS; System; Automated

The increasing interest of clinical research groups in proteomic investigations at the Core facility for protein Analysis of the LMU (Zentrallabor für Proteinanalytik, ZfP) led to the establishment of ZfP for clinical proteomics (ClinZfP). The high demand for measurements requested from clinical research groups can no longer be met with the available instruments. For this reason, two systems for the detection (EXPLORE) and validation (CONFIRM) of clinical biomarkers are requested in this and the related application.This increasing interest in clinical proteomics is also illustrated by the ZfP's membership in the recently established network of proteome laboratories in Munich (CLINSPECT-M), which is intended to meet the medical need for mass spectrometry in neurological diseases. CLINSPECT-M is one of four clusters funded by the Federal Ministry of Knowledge and Research (BMBF) in Germany to establish mass spectrometric methods in routine clinical applications (https://www.gesundheitsforschung-bmbf.de/de/clinspect- m-clinical-mass-spectrometry-center-munchen-10979.php). The increased measuring capacity that would become available by the acquisition of two mass spectrometers applied for, would allow the ClinZFP at the BMC to use the pipelines set up in CLINSPECT-M for other clinical questions and thus significantly improve proteomic research for clinical diagnostics and therapy.

随着临床研究小组对 LMU 蛋白质分析核心设施 (Zentrallabor für Proteinanalytik, ZfP) 蛋白质组学研究的兴趣日益浓厚，导致临床蛋白质组学 ZfP (ClinZfP) 的建立。现有仪器已无法满足临床研究小组对测量的高要求。因此，在此应用和相关应用中需要用于临床生物标志物检测 (EXPLORE) 和验证 (CONFIRM) 的两个系统。ZfP 在慕尼黑最近建立的蛋白质组实验室网络 (CLINSPECT-M) 中的成员资格也说明了人们对临床蛋白质组学日益增长的兴趣，该网络旨在满足神经系统疾病中质谱分析的医疗需求。 CLINSPECT-M 是德国联邦知识与研究部 (BMBF) 资助的四个集群之一，旨在在常规临床应用中建立质谱方法 (https://www.gesundheitsforschung-bmbf.de/de/clinspect-m-clinical-mass-spectrometry-center-munchen-10979.php)。通过购买所申请的两台质谱仪来提高测量能力，BMC 的 ClinZFP 可以使用 CLINSPECT-M 中建立的管道解决其他临床问题，从而显着改善临床诊断和治疗的蛋白质组学研究。