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MOLECULAR MECHANISM OF PLOIDY-SHIFT AND VARIATION OF COLONY MORPHOLOGY IN THE IMPERFECT YEASTS

MOLECULAR MECHANISM OF PLOIDY-SHIFT AND VARIATION OF COLONY MORPHOLOGY IN THE IMPERFECT YEASTS
不完全酵母倍性转变的分子机制和菌落形态变异
批准号:
06640865
负责人:
SUZUKI Takahito
金额:
$1.34万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for General Scientific Research (C)
财政年份:
1994
资助国家:
日本
项目状态:
已结题
起止时间:
1994 至 1995

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中文摘要
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英文摘要
Some strains of Candida albicans poroduce a proportion of the cell population with a higher ploidy than the rest in normal diploid state. This phenotype was named as Pld^-by the author. Ploidy-shift was observed between two different ploidy states. Pld-STN21 (arg4, Pld^-) was isolated from a weakly growing sector of a half-sectored colony, after UV-irradiation of STN14, an arg4 derivative of C.albicans CBS5736 and from the other normally growing sector, STN22 (arg4, Pld^+) was isolated. STN22, as well as its progenitor, CBS5736, showed a homogeneus colony size when plated out, but STN21 showed a characteristic heterogeneity of colony size on YPD plates at an early stage of incubation : two types of colonies, small ones and large ones, were observed.The chromosomes of these strains were separated out by PFGE.Some culture of STN21 showed the single band of chromosome 7, suggesting due to the occurrence of chromosome non-disjunction. The other culture of STN21 gave an extrachromosomal band, the size of which was larger than that of chromosome 7, and the loss of one of the two bands of chromosome 7. Southern blot hybridization experiments showed the existence of a SfiI fragment 7F,a central portion of chromosome 7, on the extrachromosomal band but the absence of a terminal SfiI fragment 7C.Protoplast fusion experiments showed, at least, two complementation groups of PLD,the function of which may stabilize both ploidy state and chromosome fidelity.
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2) Yano, S., Inoue, S., Nouchi, R., Kato, M., and Suzuki, T.: "Bioactivity of nickel (II) complex containing N-glycosides dirived from d-glucosamine and ethylenediamine against pathogenic yeast, Candida albicans." Biological & Pharmaceutical Bulletin. 18.
2) Yano, S.、Inoue, S.、Nouchi, R.、Kato, M. 和 Suzuki, T.:“含有由 d-葡萄糖胺和乙二胺衍生的 N-糖苷的镍 (II) 复合物对病原酵母的生物活性
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19
    Volatile organic compounds emitted in the headspace by fungal colonies play some communicating roles between co-cultured ones.
    • 批准号:
      25650148
    • 项目类别:
      Grant-in-Aid for Challenging Exploratory Research
    • 资助金额:
      $2.58万
    • 财政年份:
      2013
    • 负责人:
      SUZUKI Takahito
    • 依托单位:
    Analytical studies on microbial volatile organic compounds (MVOCs) and their physiological role in the microbial communities participating in the degradation of cultural properties.
    • 批准号:
      20300288
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $12.4万
    • 财政年份:
      2008
    • 负责人:
      SUZUKI Takahito
    • 依托单位:
    The role of vitamin B1 as a regulator of yeast dimorphism (yeast-hypha conversion)
    • 批准号:
      13640664
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $2.3万
    • 财政年份:
      2001
    • 负责人:
      SUZUKI Takahito
    • 依托单位:
    Characterization of genes regulating dimorphic transition between yeast and pseudohyphal form.
    • 批准号:
      10640650
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $2.3万
    • 财政年份:
      1998
    • 负责人:
      SUZUKI Takahito
    • 依托单位:
    国内基金
    海外基金
    活性代谢物 OA 调控 Hog1 介导 Candida albicans 死亡 的机制研究
    • 批准号:
      2024JJ6396
    • 项目类别:
      省市级项目
    • 资助金额:
      --
    • 批准年份:
      2024
    • 负责人:
      彭雪玲
    • 依托单位: