Characterization of genes regulating dimorphic transition between yeast and pseudohyphal form.

调节酵母和假菌丝形式之间二态性转变的基因的表征。

• 批准号: 10640650
• 负责人: SUZUKI Takahito
• 金额: $ 2.3万
• 依托单位: NARA WOMEN'S UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10640650/
• 关键词: Candida tropicalis; pseudomycelial formation; ethanol; subtractive cloning; thiamine; tyrosine phosphatase; nmt1^+ homologoue; PPL1 homologue; 酵母; Candida tropicalis; ニ形性; 菌糸形成; サブトラクション法; nmt1^+遺伝子; 二形性; nmt1 遺伝子

Ethanol caused mycelial growth in the imperfect yeast Candida tropicalis Pk233. Cultivation with ethanol gave biphasic growth curves. During the first growth phase in the ethanol culture, there was an accumulation of swollen spherical yeast cells and after entering the second phase, pseudohyphal cells appeared, projecting from the swollen yeast cells. Subtractive cloning of genes was performed on the cDNAs of the ethanol culture, from which the cDNAs of the control cultur had been subtracted by hybridization. Isolated included Ctnmt1^+ homologous to S.pombe nmt1^+ oncoding the enzyme for thiamine biosynthesis and CtPP1 homologous to C.albicans CPP1 encoding tyrosine phosphatase.. Correlation was suggested of the process of ethanol-induced pseudomycelial formation with thiamine metabolism, since the elongation of the pseudohyphal growth was suppressed when exogenous thiamine was supplemented at the late stage of the first growth phase. Disruption of CtPP1 brought about the phenotype defective in pseudomycelial formation, suggesting the participation of MAP kinase cascade in the formation.

乙醇引起的菌丝生长在不完全酵母热带假丝酵母Pk233。用乙醇培养得到双相生长曲线。在乙醇培养物的第一生长阶段，有一个肿胀的球形酵母细胞的积累，进入第二阶段后，出现假菌丝细胞，从肿胀的酵母细胞伸出。在乙醇培养物的cDNA上进行基因的消减克隆，其中对照培养物的cDNA已通过杂交从乙醇培养物的cDNA中消减。分离的包括与粟酒裂殖酵母nmt 1 ^+同源的编码硫胺素生物合成酶的Ctnmt 1 ^+和与白色念珠菌CPP 1同源的编码酪氨酸磷酸酶的CtPP 1。乙醇诱导的假菌丝体形成的过程与硫胺素代谢的相关性，因为在第一生长阶段的后期补充外源硫胺素时，假菌丝生长的伸长被抑制。CtPP 1的破坏导致假菌丝体形成的表型缺陷，表明MAP激酶级联反应参与了假菌丝体的形成。

项目成果

Sugawara T,Tanaka A,Tanaka K,Nagai K,Suzuki K & Suzuki T: "YM-170320, a novel lipopeptide antibiotic inducing morphological change of colonies in a mutant of Candida tropicalis pK233."Journal of Antibiotics. 51. 435-438 (1998)

菅原T、田中A、田中K、永井K、铃木K

Sugawara T, Tanaka A, Tanaka K, Nagai K, Suzuki K & Suzuki T.: "YM-170320, a novel lipopeptide antibiotic inducing Morphological change of colonies in a mutant of Candida tropicalis pK233."J.Antibiotics. 51. 435-438 (1998)

菅原T、田中A、田中K、永井K、铃木K

鈴木孝仁,今西由巳,岩口伸一,上原悌次郎: "「Candida tropicalisの菌糸形成に特異的な遺伝子の検索」"Journal of Medical Mycology. 39. 61-63 (1998)

Takahito Suzuki、Yoshimi Imanishi、Shinichi Iwaguchi、Teijiro Uehara：“搜索热带假丝酵母菌丝形成的基因”《医学真菌学杂志》39. 61-63 (1998)。

Suzuki T, Imanishi Y, Iwaguchi SI & Kamihara, T.: "A search for specific genes working on the process of mycelial growth in Candida tropicalis."J.Med.Mycol.. 39. 61-63 (1998)

铃木 T、今西 Y、岩口 SI

Suzuki T,Imanishi Y,Iwaguchi S-I & Kamihara T: "Depolarized cell growth precedes filamentation during the process of ethanol-induced pseudohyphal formation in the yeast Candida tropicalis." Microbiology. 144. 403-410 (1998)

铃木 T、今西 Y、岩口 S-I