Genetic regulation of dimorphic transition between yeast and hyphal form growth in the imperfect yeast, Candida tropicalis.

不完美酵母热带假丝酵母中酵母和菌丝形式生长之间二态性转变的遗传调控。

• 批准号: 08640846
• 负责人: SUZUKI Takahito
• 金额: $ 1.41万
• 依托单位: NARA WOMEN'S UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08640846/
• 关键词: Candida tropicalis; hyphal formation; ethanol; substractive cloning; NMT1; thiamine; NMTI遺伝子; チアミン; candida tropicalis; NMT1遺伝子; チロシンリン酸化酵素; リボゾームタンパク

Ethanol caused mycelial growth in the imperfect yeast Candida tropicalis Pk233. Cultivation with ethanol gave biphasic growth curves. During the first growth phase in the ethanol culture, there was an accumulation of swollen spherical yeast cells, instead of the oblong ones observed in the control culture, followed by the appearance of spherical daughter cells in chains. During the second growth phase, hyphal cells appeared, projecting from the swollen yeast cells. Subtractive cloning of genes was performed on the cDNAs of the ethanol culture, from which the cDNAs of the control cultur had been subtracted by hybridization. Isolated genes specific for the process of hyphal formation included nmt1 homolog and URP2 homolog. Correlation was suggested of the process of ethanol-induced hyphal formation with thiamine metabolism, since the mycelial growth was abolished by addition of thiamine.

乙醇引起的菌丝生长在不完全酵母热带假丝酵母Pk233。用乙醇培养得到双相生长曲线。在乙醇培养物中的第一生长期期间，存在肿胀的球形酵母细胞的积累，而不是在对照培养物中观察到的椭圆形酵母细胞，随后出现成链的球形子细胞。在第二生长期，菌丝细胞出现，从肿胀的酵母细胞中伸出。在乙醇培养物的cDNA上进行基因的消减克隆，其中对照培养物的cDNA已通过杂交从乙醇培养物的cDNA中消减。对菌丝形成过程具有特异性的分离基因包括nmt 1同源物和URP2同源物。乙醇诱导的菌丝形成过程与硫胺素代谢的相关性，因为菌丝的生长被废除，通过添加硫胺素。

项目成果

鈴木孝仁: "細胞の極性成長にはたらく共通な遺伝子像" 化学と生物. 35巻・2号. 119-120 (1997)

Takahito Suzuki：“在细胞极性生长中发挥作用的常见遗传图像”，《化学与生物学》，第 35 卷，第 2 期，119-120（1997 年）。

Yano,S,Inoue,S,Nouchi,R,Mogami K,Suzuki T: "Antifungal nickel (II) complexes derived from amino sugars against pathogenic yeast,candida albicans." Journal of Inorganic Biochemistry. 印刷中 (1998)

Yano, S, Inoue, S, Nouchi, R, Mogami K, Suzuki T：“源自氨基糖的抗真菌镍 (II) 复合物可对抗致病性酵母菌、白色念珠菌。”《无机生物化学杂志》出版中（1998 年）。

鈴木孝仁・今西由巳・岩口伸一・上原悌次郎: "Candida tropicalisの菌糸形成に特異的な遺伝子の検索" 日本医真菌学会誌. 39・(3). 発表予定 (1998)

Takahito Suzuki、Yoshimi Imanishi、Shinichi Iwaguchi、Teijiro Uehara：“搜索热带假丝酵母菌丝形成的基因”，日本医学真菌学会杂志 39·(3) 预定发表（1998 年）。

坂口修一,渋谷恭子,飯田秀利,安楽泰宏,鈴木孝仁: "Roles of Ca^<2+> in hyphal and yeast-form growths in Candida albicans Growth regulation by altered extracellular and intracellular tree Ca^<2+> conantrations." Myco science. (発表予定). (1997)

Shuichi Sakaguchi、Kyoko Shibuya、Hidetoshi Iida、Yasuhiro Anraku、Takahito Suzuki：“Ca^<2+> 在白色念珠菌菌丝和酵母形式生长中的作用通过改变细胞外和细胞内树 Ca^<2+> 浓度进行生长调节。 “真菌科学。（待提交）。（1997）

Suzuki, T., Imanishi, Y., Iwaguchi, S.& Kamihara, T.: "Depolarized growth precedes filamentatin during the process of ethanol-induced pseudohyphal formation in the yeast Candida tropicalis." Microbiology. 144. 403-410 (1998)

铃木 T.、今西 Y.、岩口 S.