Genetic regulation of dimorphic transition between yeast and hyphal form growth in the imperfect yeast, Candida tropicalis.

乙醇引起的菌丝生长在不完全酵母热带假丝酵母Pk233。用乙醇培养得到双相生长曲线。在乙醇培养物中的第一生长期期间，存在肿胀的球形酵母细胞的积累，而不是在对照培养物中观察到的椭圆形酵母细胞，随后出现成链的球形子细胞。在第二生长期，菌丝细胞出现，从肿胀的酵母细胞中伸出。在乙醇培养物的cDNA上进行基因的消减克隆，其中对照培养物的cDNA已通过杂交从乙醇培养物的cDNA中消减。对菌丝形成过程具有特异性的分离基因包括nmt 1同源物和URP2同源物。乙醇诱导的菌丝形成过程与硫胺素代谢的相关性，因为菌丝的生长被废除，通过添加硫胺素。

Ethanol caused mycelial growth in the imperfect yeast Candida tropicalis Pk233. Cultivation with ethanol gave biphasic growth curves. During the first growth phase in the ethanol culture, there was an accumulation of swollen spherical yeast cells, instead of the oblong ones observed in the control culture, followed by the appearance of spherical daughter cells in chains. During the second growth phase, hyphal cells appeared, projecting from the swollen yeast cells. Subtractive cloning of genes was performed on the cDNAs of the ethanol culture, from which the cDNAs of the control cultur had been subtracted by hybridization. Isolated genes specific for the process of hyphal formation included nmt1 homolog and URP2 homolog. Correlation was suggested of the process of ethanol-induced hyphal formation with thiamine metabolism, since the mycelial growth was abolished by addition of thiamine.