Studies on the control of steroid hormore receptor expression in chicken ovary

鸡卵巢类固醇激素受体表达调控的研究

• 批准号: 06660356
• 负责人: YOSHIMURA Yukinori
• 金额: $ 1.02万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06660356/
• 关键词: chicken; ovary; steroid hormone; hormone receptor; follicular growth; ovulation; oocyte; progesterone receptor; ステロイドホルモンレセプター; 黄体形成ホルモン; 卵胞刺激ホルモン; レセプター発現

1. Sex steroid hormones are involved in the control of ovarian functions. Our goal was to determine the mechanism of steroid hormone receptor induction in hen ovary.2. Receptors of progesterone, androgen and estrogen (PR,AR and ER) were localized in the ovary by immunocytochemistry and weatern blot analysis. Granulosa cells were positive for AR and ER in growing follicles, whereas positive for AR and PR in follicles just before ovulation. Thecal fibroblasts were positive for AR and PR in all follicles, and estrogen producing cells were positive for ER in growing follicles but not in follicle just before ovulation. These results support current reports suggesting that estrogen and progesterone play important roles for follicular growth and ovulation, respectively.3. Mechanism of PR induction in granulosa cells were examined. Luteinizing hormone (LH) and cAMP were effective to increase the granulosa cell PR in both in vivo and in vitro experiment. The results suggest that LH induces granulosa cell PR through protein kinase A pathway.4. The role of oocyte in follicular maturation was examined. The results suggested that oocyte may play a role to regulate the steroid hormone receptor in follicles becase destruction of oocyte suppressed the preovulatory increase of LH and progesterone. Also, the method of in situ hybridization for PR to detect PRmRNA expression was established.

1。性类固醇激素参与卵巢功能的控制。我们的目标是确定卵巢中类固醇激素受体诱导的机理2。孕激素，雄激素和雌激素（PR，AR和ER）的受体通过免疫细胞化学和Weatern印迹分析定位于卵巢中。颗粒细胞在卵泡中的AR和ER呈阳性，而排卵前的AR和PR为阳性。所有卵泡中的thecal成纤维细胞对AR和PR呈阳性，而产生雌激素的细胞在生长卵泡中为ER呈阳性，而在排卵前则不在卵泡中。这些结果支持当前的报告，表明雌激素和孕酮分别在卵泡生长和排卵中起重要作用。3。检查了颗粒细胞中PR诱导的机理。黄体生成激素（LH）和CAMP有效地增加体内和体外实验中的颗粒细胞PR。结果表明LH通过蛋白激酶A途径诱导颗粒细胞PR.4。研究了卵母细胞在卵泡成熟中的作用。结果表明，卵母细胞可能在卵泡中调节类固醇激素受体的作用，因为卵母细胞的破坏抑制了LH和孕酮的排卵前增加。同样，还建立了原位杂交检测PRMRNA表达的方法。

项目成果

Yoshimura,Y.,Bahr,J.M.: "Atretic changes of follicular wall caused by destruction of the germinal disc region of an immature preovulatory follicle in the chicken" Journal of Reproduction and Fertility. 105. 147-151 (1995)

Yoshimura,Y.,Bahr,J.M.：“因鸡未成熟排卵前卵泡的生发盘区域破坏而引起的卵泡壁闭锁变化”《生殖与生育杂志》。

Yoshimura Y,Okamoto T,Tamura T,: "Changes in localization of ovarian imunoreactive estrogen receptor during follicular development in hens" General and Comparative Endocrinology. 100. 368-374 (1995)

Yoshimura Y、Okamoto T、Tamura T，：“母鸡卵泡发育过程中卵巢免疫反应性雌激素受体定位的变化”普通和比较内分泌学。

Yoshimura, Y.J.M.Bahr: "Atretic changes of follicular wall caused by destruction of the germinal disc region of an immature preovulatory follicle in the chicken." Journal of Reproduction and Fertility. 105. 147-151 (1995)

Yoshimura, Y.J.M.Bahr：“鸡中未成熟排卵前卵泡的生发盘区域被破坏导致卵泡壁闭锁变化。”

Yoshimura,Y.,Okamoto,T.,Tamura,T.,: "Changes in localization of ovarian immunoreactive estrogen receptor during folliculor development in hens" General and Comparative Endocrinology. 100. 368-374 (1995)

Yoshimura，Y.，Okamoto，T.，Tamura，T.，：“母鸡卵泡发育过程中卵巢免疫反应性雌激素受体定位的变化”普通和比较内分泌学。

Terada, T.and Y.Yoshimura: Reproduction in Domestic Animals. Asakura Shoten. Co., 144-165 (1995)

Terada, T. 和 Y.Yoshimura：家养动物的繁殖。