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Endocrine control of immune response to salmonella antigen in hen oviduct

母鸡输卵管沙门氏菌抗原免疫反应的内分泌控制

基本信息

批准号：
12660257
负责人：
YOSHIMURA Yukinori
金额：
$ 2.18万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660257/
关键词：
bird salmonella reproductive immunology estrogen MHC mRNA IgG mRNA egg oviduct IgG mRNG ニワトリサルモネラ免疫卵巣生殖器内分泌

项目摘要

One of the recent problems in poultry industry is the contamination of birds and eggs by salmonella organisms. Generally, the process of immune response to foreign agents includes, antigen presentation by major histocompatibility complex (MHC) class II expressing cells, stimulation of T cells by this MHC signals followed by B cell activation, and antibody production by B cells. It is important to enhance immune functions in the reproductive organs to protect the infections and contamination of birds themselves and eggs. The aim of this study was to determine the mechanism by which immune functions in the reproductive organs are regulated by gonadal steroids and how the immune function play roles in protection of reproductive organs from salmonella antigen. The results showed that the mRNAs encoding IgG and MHC class II were expressed in the oviduct. Estrogen increased IgG mRNA expression as well as the population of T and B cells, and also it increased yolk IgY. Such enhancive effect of estrogen on immune functions occurred specifically in the target organs for estrogen. Furthermore, responsiveness of immune functions to salmonella antigen was confirmed because the injection of this antigen enhanced MHC class II expression in the ovary. These results suggested that the oviduct is the inducible site of immunoreactions that are enhanced by estrogen, and the immune response to salmonella antigen could be also induced in these organs.

家禽业最近面临的问题之一是鸟类和鸡蛋受到沙门氏菌污染。一般来说，对外来物质的免疫反应过程包括主要组织相容性复合体（MHC）II类表达细胞的抗原呈递、该MHC信号刺激T细胞、随后B细胞激活以及B细胞产生抗体。增强生殖器官的免疫功能对于防止禽类本身和禽蛋的感染和污染非常重要。本研究的目的是确定性腺类固醇调节生殖器官免疫功能的机制，以及免疫功能如何在保护生殖器官免受沙门氏菌抗原侵害中发挥作用。结果显示，输卵管中表达了编码IgG和MHC II类的mRNA。雌激素增加 IgG mRNA 表达以及 T 和 B 细胞数量，并且还增加卵黄 IgY。雌激素对免疫功能的这种增强作用专门发生在雌激素的靶器官中。此外，由于沙门氏菌抗原的注射增强了卵巢中MHC II类表达，因此证实了免疫功能对沙门氏菌抗原的反应。这些结果表明，输卵管是雌激素增强免疫反应的诱导部位，这些器官也可以诱导针对沙门氏菌抗原的免疫反应。