Molecular pathological study on the beta-amyloidogenesis in the day cerebral blood nessels.

日间脑血中β-淀粉样蛋白生成的分子病理学研究。

• 批准号: 06660402
• 负责人: SHIMADA Akinori
• 金额: $ 1.34万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06660402/
• 关键词: Dog; Brain; beta-amyloid (betaAP); Amyloid precursor protein (betaAPP); βアミロイド蛋白(βAP); βアミロイド前駆蛋白(βAPP); mRNA

beta-amyloid precursor protein (betaAPP) and beta-amyloid protein (betaAP) immunohistochemistry, and betaAPPmRNA in situ hybridization were planned in order to elucidate the beta amyloidogenesis in the dog cerebral blood vessels. 1) betaAPP staining was demonstrated in the wall of the blood vessels and occasional neurons. 2) Six cDNA fragments from the dog betaAPP mRNA were obtained by RT-PCR method. In addition, immunoelectron microscopy demonstrated 1) occasional betaAPP and betaAP immunostaining in the cytoplasm of the smooth muscles in the wall of the blood vessels ; 2) no spatial relationship was observed between the antigens and suborganellas including lysozome.

本研究拟采用β-淀粉样前体蛋白（β-APP）和β-淀粉样蛋白（β-AP）免疫组织化学方法及β-APP mRNA原位杂交技术，探讨犬脑血管β-淀粉样蛋白的形成。1)在血管壁和偶尔的神经元中证实了β APP染色。2)用RT-PCR方法从犬β APP mRNA中扩增出6个cDNA片段。此外，免疫电镜显示：1）血管壁平滑肌细胞质中偶见betaAPP和betaAP免疫染色; 2）在抗原和亚细胞器（包括溶菌酶）之间未观察到空间关系。

项目成果

Hirai, T.: "Age-related changes in the olfactory system of dogs" Neuropathology and Applied Neurobiology. (in press).

Hirai, T.：“狗嗅觉系统的年龄相关变化”神经病理学和应用神经生物学。

Hirai, T., Shimada, A., Umemura, T., Sakai, M., Itakura, C.: "Age-nelated changes in the olfactory system of dogs." Neuropathology and Applied Neurobiology. (in press).

Hirai, T.、Shimada, A.、Umemura, T.、Sakai, M.、Itakura, C.：“狗的嗅觉系统与年龄相关的变化。”

島田章則: "イヌの聴覚糸における形態的加齢性変化" Neuropathology. 14 supplements. 46 (1994)

Akinori Shimada：“狗听觉线的形态年龄相关变化”神经病理学 14 增刊 46 (1994)。

A.Shimada, M.Ebisu, T.Awakura, T.Umemura: "Age-related morphological changes in the anditory system of dogs" Neuropathology. 14 supplements. 46 (1994)

A.Shimada、M.Ebisu、T.Awakura、T.Umemura：“狗的嗅觉系统中与年龄相关的形态变化”神经病理学。