Oxidative stress and aging : significance of Metallothionein in the respiratory organs of dogs

氧化应激和衰老：金属硫蛋白在狗呼吸器官中的意义

• 批准号: 14560265
• 负责人: SHIMADA Akinori
• 金额: $ 2.3万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560265/
• 关键词: metallothionein; respiratory; dog; Suspended particulate matter; SPM; 肺; クララ細胞; SOD; 鉄

Effects of inhaled suspended particulate matters (SPM) on the human health are great concern in the world. SPM are transferred from the one continent to the other through the air (trans boundary air pollution). Out of SPM, ultra fine particles inhaled through the nostril are easily transported to the alveoli in the lung, where a variety of changes including inflammation may occur. Contents of SPM and pathological changes induced by the accumulated SPM, significance of netallothionein as a protection system against the oxidative stress induced by the SPM are yet to be elucidated. In this study, we report 1)morphological changes and 2)metal contents in the lung and hilar lymph nodes from dogs.Laminin immunohistochemistry and silver staining for the analysis of basement membrane, Prussian blue DAB and post DAB enhancement technique for the detection of iron and azan stain for the fibrosis were carried out as histological examination. Metals were determined using [CP-MS after wet digestion … More of the tissue samples. Lung and hilar lymph nodes from 7 young animals, ranging in age from 1 week to 4 years, and from 30 old animals, from 10 to 17 years, were used. Size of SPM, most of which were observed in the macrophages in the tissues, appeared to be within the range of ultra fine particles (less than 100nm). Metallothionein immunoreactivity, Fibrosis and attenuated basement membrane were demonstrated in the alveolar walls of aged dog lungs. Intensity of SPM deposition in both lung tissue and hilar lymph nodes increased with age. Signs of tissue damage by the released reactive oxygen species were also shown with the use of 8-OHdG immunohistochemistry. Age-related increase of the level of metals(Fe, Al) was demonstrated ; the pattern of the metal contents was similar to that of ambient air. These findings suggest that respiratory organs from dogs may be good biological samples for the study of the effect of SPM on the respiratory system and protection mechanism by intrinsic proteins including metallothionein. Less

可吸入悬浮颗粒物（SPM）对人体健康的影响已引起世界各国的广泛关注。悬浮颗粒物通过空气从一个大陆转移到另一个大陆（跨界空气污染）。在SPM之外，通过呼吸道吸入的超细颗粒很容易被运输到肺中的肺泡，在那里可能发生包括炎症在内的各种变化。SPM的含量和积累SPM引起的病理变化，以及netallothionein作为SPM诱导的氧化应激保护系统的意义尚未阐明。本研究采用免疫组化法、银染法、普鲁士蓝DAB及DAB后增强技术检测肺组织及肺门淋巴结的铁含量，并对肺组织及肺门淋巴结进行了病理学观察。在湿法消化后使用[CP-MS]测定金属 ...更多信息 的组织样本。使用了7只幼龄动物（年龄范围为1周至4岁）和30只老龄动物（年龄范围为10至17岁）的肺和肺门淋巴结。SPM的大小似乎在超细颗粒（小于100 nm）范围内，其中大部分在组织中的巨噬细胞中观察到。老年犬肺泡壁金属硫蛋白免疫反应阳性，纤维化，基底膜变薄。肺组织和肺门淋巴结内SPM沉积强度随年龄增长而增加。使用8-OHdG免疫组织化学也显示了由释放的活性氧引起的组织损伤的迹象。金属（铁，铝）的水平的增加与环境相关的证明，金属含量的模式是类似的环境空气。这些结果表明，狗的呼吸器官可能是一个很好的生物样品，为研究SPM对呼吸系统的影响和保护机制的内在蛋白质，包括金属硫蛋白。少

项目成果

Shimada A., Oshima, M., Sawada, M., Hasegawa, T., Seko, Y.: "Morphological changes and Metal accumulation in the lung of aged dogs"Toxicological Science. 72(supple.). 189-190 (2003)

Shimada A.、Oshima, M.、Sawada, M.、Hasekawa, T.、Seko, Y.：“老年狗肺部的形态变化和金属积累”毒理学。

Shimada A., Oshinta, M., Sawada, M., Hasegawa, T., Seko, Y.: "Morphological changes and metal accumulation in the lung of aged dogs"Toxicol.Sci.. 72 supplement. 189-190 (2003)

Shimada A.、Oshinta, M.、Sawada, M.、Hasekawa, T.、Seko, Y.：“老年狗肺部的形态变化和金属积累”Toxicol.Sci.. 72 增刊。

Shimada. A.: "MORPHOLOGICAL CHANGES AND METAL ACCUMULATION IN THE LUNG OF AGED DOGS"Toxicol. Sci. Supplement. (発表予定). (2003)

岛田：“老年狗肺部的形态变化和金属积累”，《毒理学》增刊（2003 年）。